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The map label for this gene is lhgO [H]

Identifier: 83721063

GI number: 83721063

Start: 249129

End: 250250

Strand: Direct

Name: lhgO [H]

Synonym: BTH_I0217

Alternate gene names: 83721063

Gene position: 249129-250250 (Clockwise)

Preceding gene: 83719045

Following gene: 83720124

Centisome position: 6.54

GC content: 71.21

Gene sequence:

>1122_bases
ATGACGATGGAGCGGATTGACTGTGTGGTGATCGGCGCCGGCGTTGTCGGCCTCGCGATCGCGCGCGAGCTGGCCGCGCG
CGGACGCGAGACGCTGATCCTGGAGGCGGCCGACGCGATCGGCACGGGCACGAGTTCGCGCAACAGCGAGGTGATCCACG
CGGGGCTCTATTACCCGCGCGGGTCGCTGAAGGCGTCGCTGTGCGTGCACGGGCGAGACCTGCTGTACGACTTTTGCGAC
ACGCACCAGGTGCCGCACCGGCGCTGCGGCAAGCTCGTCGTCGCGACCTCGCCCGCGCAGGCGAAGCAGTTGAAGGCGAT
CGCCGCGCGTGCGGAAGAAAACGGCGTGCTCGATCTGCTGACGCTGTCGCGCGACGAGGTGCAGGCGCTCGAGCCCGAGC
TCGAATGCCTCGAGGCGCTGTTCTCGCCGAGCACGGGCATCGTCGACAGCCATCAATTGATGCTCGCGCTGCTGGGCGAC
GCCGAGCGCGACGGCGCATCGTGCGCGCTGCGCTCGCCGGTCGATTCGATCGACGCGGCGGGCGGCCGCTTCGTCGTGCG
CACGGGCGGCGATGCGCCGACCGCGATCGAGGCCGCGTGCGTGATCAACAGCGCGGGGCTCGGCGCGCAGGCGCTCGCCA
AGCGCATCCGCGGGCTCGATTCGCGCTGGGTGCCGCCGCTCTATCTCGCGCGCGGCAATTACTTCGGCCTGTCGGGCCGC
GCGCCGTTCGCGCATCTCGTCTATCCGGTCCCGGACCGCGCCGGGCTCGGCGTCCATCTGACGCTCGATCTTGCCGGCGG
CGCGCGTTTCGGGCCCGACGTCGAATGGATCGACGCGCTGCGCTACGACGTCGATCCGCGTCGCGCCGACTCGTTCTACG
CGTCGATCCGCGCGTATTGGCCGGGATTGCCCGACGGCTCGCTTCAGCCGGCTTACGCGGGCATTCGCCCGAAAGTCTGC
GGGCCCGGCGAGCCCGCCGCCGATTTCGTGATTCAGGGTGCGGCGCAGCACGGCGTGCGCGGGCTCGTGAACCTGTTCGG
CATCGAGTCGCCCGGGCTCACCGCGGCGCTCGCGATCGCGCAGCGCGTCGGCGAAATGGCGGCGCGCACGCCGCATGCGT
AG

Upstream 100 bases:

>100_bases
CGCGGGACGCCGCCGTCCGATCGGCTTTTGACGGCCGCCACGCGCACGCGCCGCGCGACGTACAATCGAAGGCCCTGTCT
TCGAAGCGTGAGCGAAGCGA

Downstream 100 bases:

>100_bases
GTCGCGTGGAGATTTCTTGTTTTCGATCGGCTTATCTTCGGAATTTCAGGCGACACATCGGGGCGCATGAGCGGTATGCT
TGTGCGCTGCTGCCGCTAGA

Product: FAD-binding oxidoreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTMERIDCVVIGAGVVGLAIARELAARGRETLILEAADAIGTGTSSRNSEVIHAGLYYPRGSLKASLCVHGRDLLYDFCD
THQVPHRRCGKLVVATSPAQAKQLKAIAARAEENGVLDLLTLSRDEVQALEPELECLEALFSPSTGIVDSHQLMLALLGD
AERDGASCALRSPVDSIDAAGGRFVVRTGGDAPTAIEAACVINSAGLGAQALAKRIRGLDSRWVPPLYLARGNYFGLSGR
APFAHLVYPVPDRAGLGVHLTLDLAGGARFGPDVEWIDALRYDVDPRRADSFYASIRAYWPGLPDGSLQPAYAGIRPKVC
GPGEPAADFVIQGAAQHGVRGLVNLFGIESPGLTAALAIAQRVGEMAARTPHA

Sequences:

>Translated_373_residues
MTMERIDCVVIGAGVVGLAIARELAARGRETLILEAADAIGTGTSSRNSEVIHAGLYYPRGSLKASLCVHGRDLLYDFCD
THQVPHRRCGKLVVATSPAQAKQLKAIAARAEENGVLDLLTLSRDEVQALEPELECLEALFSPSTGIVDSHQLMLALLGD
AERDGASCALRSPVDSIDAAGGRFVVRTGGDAPTAIEAACVINSAGLGAQALAKRIRGLDSRWVPPLYLARGNYFGLSGR
APFAHLVYPVPDRAGLGVHLTLDLAGGARFGPDVEWIDALRYDVDPRRADSFYASIRAYWPGLPDGSLQPAYAGIRPKVC
GPGEPAADFVIQGAAQHGVRGLVNLFGIESPGLTAALAIAQRVGEMAARTPHA
>Mature_372_residues
TMERIDCVVIGAGVVGLAIARELAARGRETLILEAADAIGTGTSSRNSEVIHAGLYYPRGSLKASLCVHGRDLLYDFCDT
HQVPHRRCGKLVVATSPAQAKQLKAIAARAEENGVLDLLTLSRDEVQALEPELECLEALFSPSTGIVDSHQLMLALLGDA
ERDGASCALRSPVDSIDAAGGRFVVRTGGDAPTAIEAACVINSAGLGAQALAKRIRGLDSRWVPPLYLARGNYFGLSGRA
PFAHLVYPVPDRAGLGVHLTLDLAGGARFGPDVEWIDALRYDVDPRRADSFYASIRAYWPGLPDGSLQPAYAGIRPKVCG
PGEPAADFVIQGAAQHGVRGLVNLFGIESPGLTAALAIAQRVGEMAARTPHA

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=276, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI87082148, Length=247, Percent_Identity=34.0080971659919, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17565764, Length=281, Percent_Identity=35.5871886120996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24585081, Length=397, Percent_Identity=30.2267002518892, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 39316; Mature: 39185

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMERIDCVVIGAGVVGLAIARELAARGRETLILEAADAIGTGTSSRNSEVIHAGLYYPR
CCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCCCCEEEEEEECCC
GSLKASLCVHGRDLLYDFCDTHQVPHRRCGKLVVATSPAQAKQLKAIAARAEENGVLDLL
CCCEEEEEECCHHHHHHHHHCCCCCHHHHCEEEEEECCHHHHHHHHHHHHHCCCCCEEEE
TLSRDEVQALEPELECLEALFSPSTGIVDSHQLMLALLGDAERDGASCALRSPVDSIDAA
ECCHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCHHHHHCC
GGRFVVRTGGDAPTAIEAACVINSAGLGAQALAKRIRGLDSRWVPPLYLARGNYFGLSGR
CCEEEEEECCCCCHHHHHEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEECCCC
APFAHLVYPVPDRAGLGVHLTLDLAGGARFGPDVEWIDALRYDVDPRRADSFYASIRAYW
CCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
PGLPDGSLQPAYAGIRPKVCGPGEPAADFVIQGAAQHGVRGLVNLFGIESPGLTAALAIA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHH
QRVGEMAARTPHA
HHHHHHHHCCCCC
>Mature Secondary Structure 
TMERIDCVVIGAGVVGLAIARELAARGRETLILEAADAIGTGTSSRNSEVIHAGLYYPR
CCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCCCCEEEEEEECCC
GSLKASLCVHGRDLLYDFCDTHQVPHRRCGKLVVATSPAQAKQLKAIAARAEENGVLDLL
CCCEEEEEECCHHHHHHHHHCCCCCHHHHCEEEEEECCHHHHHHHHHHHHHCCCCCEEEE
TLSRDEVQALEPELECLEALFSPSTGIVDSHQLMLALLGDAERDGASCALRSPVDSIDAA
ECCHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCHHHHHCC
GGRFVVRTGGDAPTAIEAACVINSAGLGAQALAKRIRGLDSRWVPPLYLARGNYFGLSGR
CCEEEEEECCCCCHHHHHEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEECCCC
APFAHLVYPVPDRAGLGVHLTLDLAGGARFGPDVEWIDALRYDVDPRRADSFYASIRAYW
CCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
PGLPDGSLQPAYAGIRPKVCGPGEPAADFVIQGAAQHGVRGLVNLFGIESPGLTAALAIA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHH
QRVGEMAARTPHA
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]