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The map label for this gene is tesA [H]

Identifier: 83720994

GI number: 83720994

Start: 2403757

End: 2404455

Strand: Reverse

Name: tesA [H]

Synonym: BTH_I2130

Alternate gene names: 83720994

Gene position: 2404455-2403757 (Counterclockwise)

Preceding gene: 83720715

Following gene: 83719077

Centisome position: 63.12

GC content: 68.53

Gene sequence:

>699_bases
ATGATCGGATCGGTAATCTTGAGCATGAAACGCACATTTCACTGGAAGGTCGGCGCAACGCTCGCCGTGATCGCGGCGCT
CGCCGATCCGGCATCCGCCGCAACGAATGCCGCGCCCGCGGCGAACGCCAACGGCGGCAAGCCGGTGATCGTCGTGCTCG
GCGACAGCCTGTCGGCCGAATACGGCCTGCCGCGCGACACGGGATGGGTCGCGCTGATGCGCGCGCGCCTCGCGGCCGAG
CGAATCGATTATAGCGTCGCGAACGCGAGCATCTCCGGCGACACGACGAGCGGCGGCCGCGCACGGCTGCCTGCGGTGCT
CGCGCGGCTCAAGCCCGCCGTCGTCGTCGTCGAGCTCGGCGCGAACGACGCGCTGCGCGGCGTGCCGCTCGCCGCGACCG
AAAGCAACCTGCGCGAGATCGTCGCGAAGGCGCGGCGCGCGCGCGCGCAGGTGCTGCTCGTCGGCATGTACGTGCCGGCG
AACTACGGACCGGACTACACGCAGAAATTTCACGGCGTCTACACGACGCTGTCCAAGGAGATGCGCGTGCCGCTCGTGCC
GTTCCTGCTCGCCGGCATCGAGAACAAGCCCGACATGTTCCAGACCGACCAGATCCATCCGACGCAGGCCGCACAGCGCC
AGTTGCTCGACAACGTGTGGCCGACCCTCAAGCCGCTGTTGCGGACGTCGTCGCACTGA

Upstream 100 bases:

>100_bases
CGCGACGCTCTCGCCCGCGCGAACCGTCAGGTCGATGCCGTCGAGGATCGTCAGCTCACCGGCGGCGTCCGCGACACGCT
TGCATACATTCCGTACTTCG

Downstream 100 bases:

>100_bases
CCGCGCCGCGCCGGCATCCCGGTGCTCCGGCACTTCGGCACTTGATACGCCGATCCGTCGTCTATGCCGGGCCGGCGGAT
GCCGTTGGGCCGCCGCTTCG

Product: acyl-CoA thioesterase I

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIGSVILSMKRTFHWKVGATLAVIAALADPASAATNAAPAANANGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAE
RIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATESNLREIVAKARRARAQVLLVGMYVPA
NYGPDYTQKFHGVYTTLSKEMRVPLVPFLLAGIENKPDMFQTDQIHPTQAAQRQLLDNVWPTLKPLLRTSSH

Sequences:

>Translated_232_residues
MIGSVILSMKRTFHWKVGATLAVIAALADPASAATNAAPAANANGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAE
RIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATESNLREIVAKARRARAQVLLVGMYVPA
NYGPDYTQKFHGVYTTLSKEMRVPLVPFLLAGIENKPDMFQTDQIHPTQAAQRQLLDNVWPTLKPLLRTSSH
>Mature_232_residues
MIGSVILSMKRTFHWKVGATLAVIAALADPASAATNAAPAANANGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAE
RIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATESNLREIVAKARRARAQVLLVGMYVPA
NYGPDYTQKFHGVYTTLSKEMRVPLVPFLLAGIENKPDMFQTDQIHPTQAAQRQLLDNVWPTLKPLLRTSSH

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=183, Percent_Identity=42.6229508196721, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 24729; Mature: 24729

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGSVILSMKRTFHWKVGATLAVIAALADPASAATNAAPAANANGGKPVIVVLGDSLSAE
CCCHHHHHHHHHHEEEHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
YGLPRDTGWVALMRARLAAERIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEC
ANDALRGVPLAATESNLREIVAKARRARAQVLLVGMYVPANYGPDYTQKFHGVYTTLSKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
MRVPLVPFLLAGIENKPDMFQTDQIHPTQAAQRQLLDNVWPTLKPLLRTSSH
CCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIGSVILSMKRTFHWKVGATLAVIAALADPASAATNAAPAANANGGKPVIVVLGDSLSAE
CCCHHHHHHHHHHEEEHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
YGLPRDTGWVALMRARLAAERIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEC
ANDALRGVPLAATESNLREIVAKARRARAQVLLVGMYVPANYGPDYTQKFHGVYTTLSKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
MRVPLVPFLLAGIENKPDMFQTDQIHPTQAAQRQLLDNVWPTLKPLLRTSSH
CCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]