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The map label for this gene is oprM [H]

Identifier: 83720933

GI number: 83720933

Start: 2794578

End: 2796212

Strand: Reverse

Name: oprM [H]

Synonym: BTH_I2443

Alternate gene names: 83720933

Gene position: 2796212-2794578 (Counterclockwise)

Preceding gene: 83720423

Following gene: 83719481

Centisome position: 73.41

GC content: 73.52

Gene sequence:

>1635_bases
ATGCGTCGGCCGCGTGGTCGACATCGTTCCGCGTCGCCGCGGCGGCGCGCGCGCGGCACTGGAGATCAAGTGAAAACGAT
GATGAAACCCCTGGCCCGTCAGGGCGGGCCGCACGGTTCGCGGCGGCCGGGCGGGCTGCGGGCATGGCCGGCCGCCCTCG
CGGCCGCGCTCGTCACGGGCTGCACGCTCGCGCCGCGCTACGAGCGTCCGGCCGCGCCCGTGCCGGCGAATTATCTGCCC
GCGGCGGCGGGCGCGCCGCGCGCCGCGAGCCCGGCGGCAGCCGGCAGCGGCGCGCGCGACGCGCAGCCAGCGTCCGACGC
GCGCGGCGGCGGCAATGTGCAGGAGATGCACGATGTGCGCCTCGACGACTGGCGCGCGTACTTCACCGATCCGGCGCTGC
GCGCGCTGATCGACGCGGCGCTCGCGAACAATCGCGATCTGCGGATCGCGACGCTGCGCATCGAGGAGGCACGCGGCCTT
TACGGCGTCGCGCGCGCGGACCGGCTGCCGTCGATCGACGGCAGCCTCGGCTACGAGCGCACGCGGCTCTACGACCCGGT
GCTGCGCGAGAGCGCGACGAGCTCGCTGTATCGCGCGAGCGTCGGCTTGAGCGCGTTCGAGATCGACCTGTTCGGCCGCG
TGAAGAGCCTGTCGGACGCGGCGCTCGCCGAGTACTTCGCGACCGCCGAGGCGCAGCGCGCGGCGCGCGTCAGCCTGATC
GCCGAAGTCGCGTCCGCCTATGTGCGCGAGCGCGCGCTCGTCGACCAACTGAAGCTCGCCGAACGCACGCTCGCCGCGCG
CGACGCGACTTACGCGCTCACGCAGCGCCGCTACGCGGCGGGCACGAGCACGGCGATCGAGCTGCGCACGGCCGAGATGC
TCGTCGCGTCCGCGCGCGCATCGAAGGCGGCGCTCGAGCGCGAGCACACGCAGGCGGCGAGCGCGCTGAAGCTGCTCGCG
GGCGACTTCACGGCGGCCCTGCCCGCCGATGCGCCCGCGCTCGACGAACTCGCGGTCGCGCGGGTGTCGCCGGGGCTGTC
GTCGGACCTGCTCGAGCAGCGGCCTGACATCCGGCAGGCCGAGCAGCGGCTCGTCGCGGCGAACGCGAACATCGGCGCGG
CGCGCGCGGCGTTCTTCCCGCGCATCGCGCTCACGACCGACATCGGCTCGGTGAGCGACGCGTTCTCGGGGCTCTTCTCG
GCAGGCTCGAGCGTGTGGACGTTCGCGCCGCGGCTCACGCTGCCGATCTTCGCGGGCGGACGCAATCGCGCGAATCTCGA
TGTCGCCGACGCGCGCAAGCATATCGCGGTCGCCGAATACGAAAAGACGATCCAGACGGCGTTCCGCGAAGTGGCCGACG
CGCTCGCCGCGCGCGATCAGATCGATGCGCAGCTCGCCGCGCAGCAGGCGGTCTACGGCGCGGACGCCGAGCGGCTGCGG
CTCGCGGAGCGGCGCTACGGCAGCGGCGTCGCAAGCTATCTCGAATTGCTCGACGCGCAGCGCAGCACGTTCGAGTCCGG
GCAGGAACTGATTCGCCTGAAGCAGTTGCGGCTCACGAACGCCATCACGCTGTATCGCGCGCTCGGCGGCGGCTGGTCGC
GCGCCGCGTGCGGCGGCGAAGACGCGTGTGCGTGA

Upstream 100 bases:

>100_bases
GCGCCGCGTCCGGCGCGCAGATCGCGATCGGCACCGGCGTGCTCGGCGGCGTGATCAGCGCGACGCTGTTCGCGATCTTC
CTCGTCCCGCTCTTTTTCGT

Downstream 100 bases:

>100_bases
ACGCGCCGCACGCGCTCATCGGCGGCGATGGCGCGCCGTTCGTCTTCGTTCGCATGAGGTGACGAAGCGGCCGGCCGCTG
GCGCGACGGCGGCGATCGGT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MRRPRGRHRSASPRRRARGTGDQVKTMMKPLARQGGPHGSRRPGGLRAWPAALAAALVTGCTLAPRYERPAAPVPANYLP
AAAGAPRAASPAAAGSGARDAQPASDARGGGNVQEMHDVRLDDWRAYFTDPALRALIDAALANNRDLRIATLRIEEARGL
YGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGLSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARVSLI
AEVASAYVRERALVDQLKLAERTLAARDATYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLA
GDFTAALPADAPALDELAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDIGSVSDAFSGLFS
AGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLR
LAERRYGSGVASYLELLDAQRSTFESGQELIRLKQLRLTNAITLYRALGGGWSRAACGGEDACA

Sequences:

>Translated_544_residues
MRRPRGRHRSASPRRRARGTGDQVKTMMKPLARQGGPHGSRRPGGLRAWPAALAAALVTGCTLAPRYERPAAPVPANYLP
AAAGAPRAASPAAAGSGARDAQPASDARGGGNVQEMHDVRLDDWRAYFTDPALRALIDAALANNRDLRIATLRIEEARGL
YGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGLSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARVSLI
AEVASAYVRERALVDQLKLAERTLAARDATYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLA
GDFTAALPADAPALDELAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDIGSVSDAFSGLFS
AGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLR
LAERRYGSGVASYLELLDAQRSTFESGQELIRLKQLRLTNAITLYRALGGGWSRAACGGEDACA
>Mature_544_residues
MRRPRGRHRSASPRRRARGTGDQVKTMMKPLARQGGPHGSRRPGGLRAWPAALAAALVTGCTLAPRYERPAAPVPANYLP
AAAGAPRAASPAAAGSGARDAQPASDARGGGNVQEMHDVRLDDWRAYFTDPALRALIDAALANNRDLRIATLRIEEARGL
YGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGLSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARVSLI
AEVASAYVRERALVDQLKLAERTLAARDATYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLA
GDFTAALPADAPALDELAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDIGSVSDAFSGLFS
AGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLR
LAERRYGSGVASYLELLDAQRSTFESGQELIRLKQLRLTNAITLYRALGGGWSRAACGGEDACA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=483, Percent_Identity=41.2008281573499, Blast_Score=345, Evalue=6e-96,
Organism=Escherichia coli, GI2367348, Length=429, Percent_Identity=26.8065268065268, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 58047; Mature: 58047

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPRGRHRSASPRRRARGTGDQVKTMMKPLARQGGPHGSRRPGGLRAWPAALAAALVTG
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
CTLAPRYERPAAPVPANYLPAAAGAPRAASPAAAGSGARDAQPASDARGGGNVQEMHDVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
LDDWRAYFTDPALRALIDAALANNRDLRIATLRIEEARGLYGVARADRLPSIDGSLGYER
HHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEHHHCCCHHHHHHCCCCCCCCCCCCHH
TRLYDPVLRESATSSLYRASVGLSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARVSLI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVASAYVRERALVDQLKLAERTLAARDATYALTQRRYAAGTSTAIELRTAEMLVASARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
SKAALEREHTQAASALKLLAGDFTAALPADAPALDELAVARVSPGLSSDLLEQRPDIRQA
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHHH
EQRLVAANANIGAARAAFFPRIALTTDIGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGG
HHHHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCEEEEEEECC
RNRANLDVADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAERRYGSGVASYLELLDAQRSTFESGQELIRLKQLRLTNAITLYRALGGGWSRAACGGE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DACA
CCCC
>Mature Secondary Structure
MRRPRGRHRSASPRRRARGTGDQVKTMMKPLARQGGPHGSRRPGGLRAWPAALAAALVTG
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
CTLAPRYERPAAPVPANYLPAAAGAPRAASPAAAGSGARDAQPASDARGGGNVQEMHDVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
LDDWRAYFTDPALRALIDAALANNRDLRIATLRIEEARGLYGVARADRLPSIDGSLGYER
HHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEHHHCCCHHHHHHCCCCCCCCCCCCHH
TRLYDPVLRESATSSLYRASVGLSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARVSLI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVASAYVRERALVDQLKLAERTLAARDATYALTQRRYAAGTSTAIELRTAEMLVASARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
SKAALEREHTQAASALKLLAGDFTAALPADAPALDELAVARVSPGLSSDLLEQRPDIRQA
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHHH
EQRLVAANANIGAARAAFFPRIALTTDIGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGG
HHHHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCEEEEEEECC
RNRANLDVADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAERRYGSGVASYLELLDAQRSTFESGQELIRLKQLRLTNAITLYRALGGGWSRAACGGE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DACA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]