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The map label for this gene is tolB [H]

Identifier: 83720845

GI number: 83720845

Start: 1550210

End: 1551601

Strand: Reverse

Name: tolB [H]

Synonym: BTH_I1373

Alternate gene names: 83720845

Gene position: 1551601-1550210 (Counterclockwise)

Preceding gene: 83720837

Following gene: 83719210

Centisome position: 40.73

GC content: 63.94

Gene sequence:

>1392_bases
TTGCGCTTACACTCCCGCCGTCGCACCGGGAACGAATCGGGTATTTTTGAGTCTCTGCGGTTGCGCAGCGTACATACGGG
AACAGGAAGCATGAGTTTGATGACGAAGCTAGGTTTCAGGGCACTGGTGGCCTCGTGTCTGATTGCCGCGGGCGGTGCCG
CCCACGCGCAGGTGAATGTCCTGATCACAGGCGTCGGCTCCACCCAGTTTCCGATCGCCACCGCGAATTTCGTCAACGAA
GCGAGTCTGCCGCAACAGGTCACGTCGATCGTGCGCGCCGACCTCGCGAGAAGCGGCAAATTCACCAACGTCGACGCGGG
CGGCACGCCCGTGCCGGAAACGGCGTCGGTCGATTTCGGCGCGTGGAAGGCGAAAGGCGCGAATGCGTTCGTCGCGGGCA
GCGTGAGCCGCGAGCCGAACGGCCAGTACAAGGTCAACTTCATCCTCTACGACACCGTCAAGCAGCAAAGCCTCGGAGGG
CTGTCGCTGACGACGTCGAACGACAACGAGGGGATGCGCAAGACCGGCCACAAGATCGCCGACTACATCTACCAGAAGCT
GCTCGGCGTGCGCGGCGTCTTCAACACGCGCCTGTCGTACGTGCAGCGCACGGGCAACGTCTACAAGCTGCTGATCTCCG
ATTCGGACGGCCAGAACGCGATTCCCGCGCTGACGAGCAAGGAACCGATCATCTCGCCCGCGTGGTCGCCGAGCGGAACG
AAGGTGGCGTACGTGTCGTTCGAGCTGAGGAAGCCCGTCGTGTACATTCACGATCTGCCGACGGGCCGCCGCTACGTGAT
CTCGAACCAGAAAGGCAACAACAGCGCACCCGCATGGTCGCCGGACGGTCAAACGCTCGCCGTCGCGCTGTCGCTCACGG
GCAATACGCAGATCTATTCCGTCAGCTCGACAGGCAGCGGGCTGCACCGCCTGACGCGCAGCAGTTCGATCGACACCGAG
CCGTTCTACTCCCCTGACGGCAAGTGGATCTATTTCACGAGCGATCGCGGCGGCGCGCCGCAGATCTATCGGATGCCCGC
CGGAGGCGAGAGCGCTGGCGCCGCTCAGCGCGTGACGTTTACCGGCAGCTACAATACGAGTCCGCGCATCAGCCCGGACG
GCAAGCTGCTTGCGTACATCTCGCGCACGGGCGGGGGCTTCAAGCTGTACGTTCAGGATCTGCAAACGGGTGCGGCGAAT
GCCGTCACCAACACGACGCGCGACGAATCGCCGAGCTTCGCGGCGAACGGTCAGTACATTCTGTACGCTACGCAATCGGG
GGGCCGCAGCGTGCTGGCTGCCGTTCCGTCCGACGGCAGCGCGCCGCCGCAGATCTTGTCCGTTCAGGGCGGCGCGATTC
GCGAGCCGTCCTGGGGGCCCTTCATGCAATGA

Upstream 100 bases:

>100_bases
CATCAGCGCGATCCAGGCATCGGTGCCCCTGCCGCCCGATACCAACGGTCGCACCCCATCCGAGATTACGATTACCTTCA
AGGCGGCGGAATGAGCGCGT

Downstream 100 bases:

>100_bases
CCACAAGGAGAGTAACCATGATGTCGAAAAAACTTCGTCTCGCGTTCGCGATGCTGATGATCGGCGCGCTCGCCGCGTGC
AAGTCGGGGGTGAAGCTCGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRLHSRRRTGNESGIFESLRLRSVHTGTGSMSLMTKLGFRALVASCLIAAGGAAHAQVNVLITGVGSTQFPIATANFVNE
ASLPQQVTSIVRADLARSGKFTNVDAGGTPVPETASVDFGAWKAKGANAFVAGSVSREPNGQYKVNFILYDTVKQQSLGG
LSLTTSNDNEGMRKTGHKIADYIYQKLLGVRGVFNTRLSYVQRTGNVYKLLISDSDGQNAIPALTSKEPIISPAWSPSGT
KVAYVSFELRKPVVYIHDLPTGRRYVISNQKGNNSAPAWSPDGQTLAVALSLTGNTQIYSVSSTGSGLHRLTRSSSIDTE
PFYSPDGKWIYFTSDRGGAPQIYRMPAGGESAGAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQTGAAN
AVTNTTRDESPSFAANGQYILYATQSGGRSVLAAVPSDGSAPPQILSVQGGAIREPSWGPFMQ

Sequences:

>Translated_463_residues
MRLHSRRRTGNESGIFESLRLRSVHTGTGSMSLMTKLGFRALVASCLIAAGGAAHAQVNVLITGVGSTQFPIATANFVNE
ASLPQQVTSIVRADLARSGKFTNVDAGGTPVPETASVDFGAWKAKGANAFVAGSVSREPNGQYKVNFILYDTVKQQSLGG
LSLTTSNDNEGMRKTGHKIADYIYQKLLGVRGVFNTRLSYVQRTGNVYKLLISDSDGQNAIPALTSKEPIISPAWSPSGT
KVAYVSFELRKPVVYIHDLPTGRRYVISNQKGNNSAPAWSPDGQTLAVALSLTGNTQIYSVSSTGSGLHRLTRSSSIDTE
PFYSPDGKWIYFTSDRGGAPQIYRMPAGGESAGAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQTGAAN
AVTNTTRDESPSFAANGQYILYATQSGGRSVLAAVPSDGSAPPQILSVQGGAIREPSWGPFMQ
>Mature_463_residues
MRLHSRRRTGNESGIFESLRLRSVHTGTGSMSLMTKLGFRALVASCLIAAGGAAHAQVNVLITGVGSTQFPIATANFVNE
ASLPQQVTSIVRADLARSGKFTNVDAGGTPVPETASVDFGAWKAKGANAFVAGSVSREPNGQYKVNFILYDTVKQQSLGG
LSLTTSNDNEGMRKTGHKIADYIYQKLLGVRGVFNTRLSYVQRTGNVYKLLISDSDGQNAIPALTSKEPIISPAWSPSGT
KVAYVSFELRKPVVYIHDLPTGRRYVISNQKGNNSAPAWSPDGQTLAVALSLTGNTQIYSVSSTGSGLHRLTRSSSIDTE
PFYSPDGKWIYFTSDRGGAPQIYRMPAGGESAGAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQTGAAN
AVTNTTRDESPSFAANGQYILYATQSGGRSVLAAVPSDGSAPPQILSVQGGAIREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=445, Percent_Identity=36.6292134831461, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 49183; Mature: 49183

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHSRRRTGNESGIFESLRLRSVHTGTGSMSLMTKLGFRALVASCLIAAGGAAHAQVNV
CCCCCCCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
LITGVGSTQFPIATANFVNEASLPQQVTSIVRADLARSGKFTNVDAGGTPVPETASVDFG
EEEECCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCC
AWKAKGANAFVAGSVSREPNGQYKVNFILYDTVKQQSLGGLSLTTSNDNEGMRKTGHKIA
CEECCCCCEEEEECCCCCCCCCEEEEEEEEECCHHHCCCCEEEEECCCCCCHHHHHHHHH
DYIYQKLLGVRGVFNTRLSYVQRTGNVYKLLISDSDGQNAIPALTSKEPIISPAWSPSGT
HHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
KVAYVSFELRKPVVYIHDLPTGRRYVISNQKGNNSAPAWSPDGQTLAVALSLTGNTQIYS
EEEEEEEEECCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEE
VSSTGSGLHRLTRSSSIDTEPFYSPDGKWIYFTSDRGGAPQIYRMPAGGESAGAAQRVTF
ECCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEE
TGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQTGAANAVTNTTRDESPSFAANGQYI
ECCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHCCCCCCCCCCEECCCCEE
LYATQSGGRSVLAAVPSDGSAPPQILSVQGGAIREPSWGPFMQ
EEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLHSRRRTGNESGIFESLRLRSVHTGTGSMSLMTKLGFRALVASCLIAAGGAAHAQVNV
CCCCCCCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
LITGVGSTQFPIATANFVNEASLPQQVTSIVRADLARSGKFTNVDAGGTPVPETASVDFG
EEEECCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCC
AWKAKGANAFVAGSVSREPNGQYKVNFILYDTVKQQSLGGLSLTTSNDNEGMRKTGHKIA
CEECCCCCEEEEECCCCCCCCCEEEEEEEEECCHHHCCCCEEEEECCCCCCHHHHHHHHH
DYIYQKLLGVRGVFNTRLSYVQRTGNVYKLLISDSDGQNAIPALTSKEPIISPAWSPSGT
HHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
KVAYVSFELRKPVVYIHDLPTGRRYVISNQKGNNSAPAWSPDGQTLAVALSLTGNTQIYS
EEEEEEEEECCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEE
VSSTGSGLHRLTRSSSIDTEPFYSPDGKWIYFTSDRGGAPQIYRMPAGGESAGAAQRVTF
ECCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEE
TGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQTGAANAVTNTTRDESPSFAANGQYI
ECCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHCCCCCCCCCCEECCCCEE
LYATQSGGRSVLAAVPSDGSAPPQILSVQGGAIREPSWGPFMQ
EEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA