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The map label for this gene is selU [H]

Identifier: 83720839

GI number: 83720839

Start: 709442

End: 710521

Strand: Direct

Name: selU [H]

Synonym: BTH_I0620

Alternate gene names: 83720839

Gene position: 709442-710521 (Clockwise)

Preceding gene: 83718985

Following gene: 83720516

Centisome position: 18.62

GC content: 68.33

Gene sequence:

>1080_bases
TTGAATCTCCAACCTGTTTCCCTCGACCAGATCGACGATTTCGACGAGATCGTCGACGTCCGCACGCCGCTCGAATACGC
GGAGGACCACATTCCGGGCGCGCTGAACGCGCCGGTTCTCAGCAACGAGGAGCGCGTGATCGTCGGCACGATGTACAAGC
AGGTGTCGCCGTTCGAGGCGACGCGCATCGGCGCGGCAATGGTCGCGCGCAACATCGCCGCGCATCTCGACACGACGTTC
GCCGACCGTCCGCGCAACTGGCGGCCGCTCATCTATTGCTGGCGCGGCGGCAAGCGTTCCGGATCGATGACGGTCTGGTT
CAACATGATCGGCTGGCGCGCGCGGCAGCTCGACGGCGGCTACAAGACGTATCGCCGTTCGGTTGTCGACGCGCTCGGCC
AACTGCCCGCCGCGTTCCGCTATGTCGTGCTGGCGGGGCATACGGGAAGCGGCAAGACGCGGCTGCTGCACGCGCTCGCG
CAGGCGGGCGCGCAGACGCTCGATCTCGAGGCGCTCGCCGCGCACCGCGGTTCGCTGCTCGGCGCGCTGCCGAGCGGCGA
GCAGCCGTCGCAGAAGGCCTTCGATACGGCGCTCGTCGGCGCGCTGCGCGGCTTCGATCGCGAACGGCCGGTGTTCATCG
AAGCGGAGAGCCGGCGCATCGGCGCGATCACGCTGCCGCTCGCGCTGCTGACCGGCATGCACGCGGCGGATTGCGTGAAT
GTCGAGACCGCGCGCGAAGAGCGCGTCGAGCTGTTGTTGCAGGACTACGGGCATCTGTTCGATCAGACCGCGTTCTTCAA
GGTCCAGCTCGAGAAGCTCGTGCCGCTGCATGGGCATGCGCGGATACGCAAGTGGCACGAGATGCTCGACGAAGGGCGGC
GCAGCGAGCTGTTCGAGGAACTCGTCGACGTTCACTACGACCCCGCCTATACGCGCAGCAGCGGCGCGCACTTCACCCGC
CTGCCGCAGGCCGAGCGTTTCCCGTTCCGGCCGACCGCGCGCGACGTCGTCGAGCAGGCGCGGGCTTTGCTCGCGCATCT
CGATGCGGCGGGCGCGAGCGCCTGCGGCAAGCGGGCCTGA

Upstream 100 bases:

>100_bases
CGGCGGCCGTGCCCGTTCGACCGCGTTGGGCGGCGTGCCGCGCGTGAGATAATCGCGCTTCCACCGCCAGCGATGATGCG
GCCCCGCCGCGCCCGATACG

Downstream 100 bases:

>100_bases
TCGGCGCATCGCCCGAATGCGGCACGCGGTGCGTGCGGTTCGAGATGGGCGCTGCATCGTGCGAGGCGATCGTTGTCGCG
GCCGCCGTGCTTCGTGTCGC

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNLQPVSLDQIDDFDEIVDVRTPLEYAEDHIPGALNAPVLSNEERVIVGTMYKQVSPFEATRIGAAMVARNIAAHLDTTF
ADRPRNWRPLIYCWRGGKRSGSMTVWFNMIGWRARQLDGGYKTYRRSVVDALGQLPAAFRYVVLAGHTGSGKTRLLHALA
QAGAQTLDLEALAAHRGSLLGALPSGEQPSQKAFDTALVGALRGFDRERPVFIEAESRRIGAITLPLALLTGMHAADCVN
VETAREERVELLLQDYGHLFDQTAFFKVQLEKLVPLHGHARIRKWHEMLDEGRRSELFEELVDVHYDPAYTRSSGAHFTR
LPQAERFPFRPTARDVVEQARALLAHLDAAGASACGKRA

Sequences:

>Translated_359_residues
MNLQPVSLDQIDDFDEIVDVRTPLEYAEDHIPGALNAPVLSNEERVIVGTMYKQVSPFEATRIGAAMVARNIAAHLDTTF
ADRPRNWRPLIYCWRGGKRSGSMTVWFNMIGWRARQLDGGYKTYRRSVVDALGQLPAAFRYVVLAGHTGSGKTRLLHALA
QAGAQTLDLEALAAHRGSLLGALPSGEQPSQKAFDTALVGALRGFDRERPVFIEAESRRIGAITLPLALLTGMHAADCVN
VETAREERVELLLQDYGHLFDQTAFFKVQLEKLVPLHGHARIRKWHEMLDEGRRSELFEELVDVHYDPAYTRSSGAHFTR
LPQAERFPFRPTARDVVEQARALLAHLDAAGASACGKRA
>Mature_359_residues
MNLQPVSLDQIDDFDEIVDVRTPLEYAEDHIPGALNAPVLSNEERVIVGTMYKQVSPFEATRIGAAMVARNIAAHLDTTF
ADRPRNWRPLIYCWRGGKRSGSMTVWFNMIGWRARQLDGGYKTYRRSVVDALGQLPAAFRYVVLAGHTGSGKTRLLHALA
QAGAQTLDLEALAAHRGSLLGALPSGEQPSQKAFDTALVGALRGFDRERPVFIEAESRRIGAITLPLALLTGMHAADCVN
VETAREERVELLLQDYGHLFDQTAFFKVQLEKLVPLHGHARIRKWHEMLDEGRRSELFEELVDVHYDPAYTRSSGAHFTR
LPQAERFPFRPTARDVVEQARALLAHLDAAGASACGKRA

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=244, Percent_Identity=35.655737704918, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 39930; Mature: 39930

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQPVSLDQIDDFDEIVDVRTPLEYAEDHIPGALNAPVLSNEERVIVGTMYKQVSPFEA
CCCCCCCHHHCCCHHHHHHHCCCHHHHHHCCCCCCCCCEECCCCEEEEEEHHHHCCCHHH
TRIGAAMVARNIAAHLDTTFADRPRNWRPLIYCWRGGKRSGSMTVWFNMIGWRARQLDGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEHHCCHHHHCCCH
YKTYRRSVVDALGQLPAAFRYVVLAGHTGSGKTRLLHALAQAGAQTLDLEALAAHRGSLL
HHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCEE
GALPSGEQPSQKAFDTALVGALRGFDRERPVFIEAESRRIGAITLPLALLTGMHAADCVN
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHCC
VETAREERVELLLQDYGHLFDQTAFFKVQLEKLVPLHGHARIRKWHEMLDEGRRSELFEE
CHHHHHHHHHHHHHHHHHHHCCCHHHEEEHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
LVDVHYDPAYTRSSGAHFTRLPQAERFPFRPTARDVVEQARALLAHLDAAGASACGKRA
HHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNLQPVSLDQIDDFDEIVDVRTPLEYAEDHIPGALNAPVLSNEERVIVGTMYKQVSPFEA
CCCCCCCHHHCCCHHHHHHHCCCHHHHHHCCCCCCCCCEECCCCEEEEEEHHHHCCCHHH
TRIGAAMVARNIAAHLDTTFADRPRNWRPLIYCWRGGKRSGSMTVWFNMIGWRARQLDGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEHHCCHHHHCCCH
YKTYRRSVVDALGQLPAAFRYVVLAGHTGSGKTRLLHALAQAGAQTLDLEALAAHRGSLL
HHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCEE
GALPSGEQPSQKAFDTALVGALRGFDRERPVFIEAESRRIGAITLPLALLTGMHAADCVN
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHCC
VETAREERVELLLQDYGHLFDQTAFFKVQLEKLVPLHGHARIRKWHEMLDEGRRSELFEE
CHHHHHHHHHHHHHHHHHHHCCCHHHEEEHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
LVDVHYDPAYTRSSGAHFTRLPQAERFPFRPTARDVVEQARALLAHLDAAGASACGKRA
HHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA