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The map label for this gene is trmJ [H]

Identifier: 83720786

GI number: 83720786

Start: 2181126

End: 2182142

Strand: Direct

Name: trmJ [H]

Synonym: BTH_I1935

Alternate gene names: 83720786

Gene position: 2181126-2182142 (Clockwise)

Preceding gene: 83719541

Following gene: 83721033

Centisome position: 57.26

GC content: 71.68

Gene sequence:

>1017_bases
TTGCGCGTGGGGCGCGCGTGCCGCTCGATCGTCCGTTCGGACGAGTGGGTTTGTCGGCGAGTTGGCGATACCATACCGGC
ATCCCGATCAGTCGAAATGAACGTTTTGGAATCTCAGCAGAAACCGTCCGCGCCGTTCGCCGGCGCGGCCACATCCCGTC
CGGCGCGGGCCGCGCGCGGCGGATTCACGTCGACGCGCTTCGTGCTCGTCGAGCCTAGCCACCCCGGCAACGTCGGTGCG
GCGGCGCGCGCGCTGAAGACGATGGGCTTTTCGCGCCTCGTGCTCGTCGCGCCGCGCGTGCCGTGCGTGCAGAGCGATCC
GGAGGCGCTCGCGATGGCGAGCGGCGCCGACGACGTGCTCGCGTCCGCGCACGTCGTGCCGACGCTCGCCGACGCGCTGA
ACGGCGTGCAATGGTCGCTCGCGCTCACCGCGCGCTCGCGCGAGTACGGGCCGCCGCGGCTCGCGCCCCGCGCGGCCGCC
GCGAGCGCGCTCCAGCAGGTGCGCACGGGCGACATCGCGTTCGTGTTCGGCAACGAGCGCACCGGCTTGTCGAACGAGCA
CGTCGAGCGTTGCAGCGCGATCGCGCATATTCCGGCGAACCCCGCTTACAGCTCGCTCAATCTCGCGCAGGCGGTGCAGG
TGCTCGCGTACGAGCTGCGCGTCGCGCTGCTCGAAGACGGCGAGCCCGCCACGCCGCCGGACGCCGCGCTCGGCGCGCGC
GCGCAAAGCGACGAGATCGAGCGGATGTTCGTTCATCTCGAAAATGCGCTGATCGCGCTCGACTTCCTCGATCCGCGCAA
TCCGAAGAAGCTGATGCCGCGGCTGCGGCGGCTCTTCGCGCGAACGGGGCTCGAACGCGAGGAAGTGAACATCGTTCGCG
GGATCGCGAAGCACATCTTGCTGAGGGCGGGCGGCACGGCCGCGCGCGCGGGCGCCTCGGGCGACGGCGAAAGCGACCCG
GGCGACGCGGAGCGTTCCGGCCGCGACGCCGGGCGAGGGGCCGGACGGGATGGCTGA

Upstream 100 bases:

>100_bases
GAAGACGAATTGGCAAAGAGCGGGATGCGGGCCGAACAGCGCGAGCGCCGCATGCGAAGACGCAATTTTACCCGACTCGC
GGCATTTTTTTGCGAAACGG

Downstream 100 bases:

>100_bases
CGCGGCGCGGGGCCCCGTGGCCCCGCGTGCTATACCCGCGGCCGCAGCAGGGAGAACGCGGCGCCCGCAGCGTGCTCGCG
GCGGCGGTTCTACAATCGTC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRVGRACRSIVRSDEWVCRRVGDTIPASRSVEMNVLESQQKPSAPFAGAATSRPARAARGGFTSTRFVLVEPSHPGNVGA
AARALKTMGFSRLVLVAPRVPCVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAA
ASALQQVRTGDIAFVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDGEPATPPDAALGAR
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHILLRAGGTAARAGASGDGESDP
GDAERSGRDAGRGAGRDG

Sequences:

>Translated_338_residues
MRVGRACRSIVRSDEWVCRRVGDTIPASRSVEMNVLESQQKPSAPFAGAATSRPARAARGGFTSTRFVLVEPSHPGNVGA
AARALKTMGFSRLVLVAPRVPCVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAA
ASALQQVRTGDIAFVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDGEPATPPDAALGAR
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHILLRAGGTAARAGASGDGESDP
GDAERSGRDAGRGAGRDG
>Mature_338_residues
MRVGRACRSIVRSDEWVCRRVGDTIPASRSVEMNVLESQQKPSAPFAGAATSRPARAARGGFTSTRFVLVEPSHPGNVGA
AARALKTMGFSRLVLVAPRVPCVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAA
ASALQQVRTGDIAFVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDGEPATPPDAALGAR
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHILLRAGGTAARAGASGDGESDP
GDAERSGRDAGRGAGRDG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=247, Percent_Identity=42.914979757085, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=38.6075949367089, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 35772; Mature: 35772

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGRACRSIVRSDEWVCRRVGDTIPASRSVEMNVLESQQKPSAPFAGAATSRPARAARG
CCCCHHHHHHHHCCHHHHHHHCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCHHHHCC
GFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPCVQSDPEALAMASGADDVL
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCCHHHH
ASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAAASALQQVRTGDIAFVFGNER
HHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
TGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDGEPATPPDAALGAR
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHCCC
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHIL
CCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LRAGGTAARAGASGDGESDPGDAERSGRDAGRGAGRDG
HHCCCCHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRVGRACRSIVRSDEWVCRRVGDTIPASRSVEMNVLESQQKPSAPFAGAATSRPARAARG
CCCCHHHHHHHHCCHHHHHHHCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCHHHHCC
GFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPCVQSDPEALAMASGADDVL
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCCHHHH
ASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAAASALQQVRTGDIAFVFGNER
HHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
TGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDGEPATPPDAALGAR
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHCCC
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHIL
CCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LRAGGTAARAGASGDGESDPGDAERSGRDAGRGAGRDG
HHCCCCHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA