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The map label for this gene is rsmD [H]

Identifier: 83720763

GI number: 83720763

Start: 522981

End: 523670

Strand: Direct

Name: rsmD [H]

Synonym: BTH_I0468

Alternate gene names: 83720763

Gene position: 522981-523670 (Clockwise)

Preceding gene: 83719191

Following gene: 83721501

Centisome position: 13.73

GC content: 71.3

Gene sequence:

>690_bases
GTGCGTCGCGCCGTTTCAGCCGGTTCATTCCGTTACCAACGCTTCCGAATCGAATTCTCTCGTATGTCCCGTTCCTCTCC
GGCGCGGCCGCAGGCGCAGCGAGCCCCAGTGGCCGGCCGCGGCAAGCCGCACACGATCCGCATCATCGGCGGCGACTGGA
AGCGCACGCCGCTTCCGGTGCTCGACCTGGACGGCCTGCGCCCGACGCCCGACCGCGTGCGCGAGACGCTCTTCAACTGG
CTTGGCCAGGATCTCGAAGGGCGGCGCTGCCTCGATCTGTTCGCCGGCAGCGGCGCGCTCGGCTTCGAGGCGGCGTCGCG
CGGCGCGGCGAGCGTCGTGATGGTCGAGCGCCATCCGCGCGCCGCCCAGCAGTTGCGCGCGCTGAAGGACAAGCTCGCCG
CGCGCGCGATCGAGATCGCGGAGGCCGATGCGCTGCGCATCGCGGCGGGCCTCGCGCCGCGCTCGTTCGACGTCGTGTTC
GTCGATCCGCCGTTCGGCGACGCGGCCGCGTTCGCGCGCGCGCTGGAGCTCGCGCCGAATCTCGTCGCGCCGGGCGGCCA
TCTGTACGTCGAGGCGGGCGCGCCGCTCGATCCGTGCGGGCACGCGTCGCTTGCCGGCTGGCGGGTTGTGCGACACGGCA
AGGCGGGCGCCGTCCACTATCATTTGCTGCAACGCGAAAATGATGAATAA

Upstream 100 bases:

>100_bases
TCGCGCGCTGCAAACCCGGCATTTTATCAGGCGCTCGCGCTTCGCCGTGCGGCGGCGGCATGGCGGGCGCTTCGCTTGTG
GTAACGTGCGCGTCATCGCG

Downstream 100 bases:

>100_bases
TGCGCGTTCCAAACGAGGCGCCGCGCAGTCGCGACGTGCGTCCATGTCGGCGGCCGCGCGCGATGCGCATGGCCGTGACC
CCAATTGCTCGATGACAGGA

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRRAVSAGSFRYQRFRIEFSRMSRSSPARPQAQRAPVAGRGKPHTIRIIGGDWKRTPLPVLDLDGLRPTPDRVRETLFNW
LGQDLEGRRCLDLFAGSGALGFEAASRGAASVVMVERHPRAAQQLRALKDKLAARAIEIAEADALRIAAGLAPRSFDVVF
VDPPFGDAAAFARALELAPNLVAPGGHLYVEAGAPLDPCGHASLAGWRVVRHGKAGAVHYHLLQRENDE

Sequences:

>Translated_229_residues
MRRAVSAGSFRYQRFRIEFSRMSRSSPARPQAQRAPVAGRGKPHTIRIIGGDWKRTPLPVLDLDGLRPTPDRVRETLFNW
LGQDLEGRRCLDLFAGSGALGFEAASRGAASVVMVERHPRAAQQLRALKDKLAARAIEIAEADALRIAAGLAPRSFDVVF
VDPPFGDAAAFARALELAPNLVAPGGHLYVEAGAPLDPCGHASLAGWRVVRHGKAGAVHYHLLQRENDE
>Mature_229_residues
MRRAVSAGSFRYQRFRIEFSRMSRSSPARPQAQRAPVAGRGKPHTIRIIGGDWKRTPLPVLDLDGLRPTPDRVRETLFNW
LGQDLEGRRCLDLFAGSGALGFEAASRGAASVVMVERHPRAAQQLRALKDKLAARAIEIAEADALRIAAGLAPRSFDVVF
VDPPFGDAAAFARALELAPNLVAPGGHLYVEAGAPLDPCGHASLAGWRVVRHGKAGAVHYHLLQRENDE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=191, Percent_Identity=42.9319371727749, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 24894; Mature: 24894

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAVSAGSFRYQRFRIEFSRMSRSSPARPQAQRAPVAGRGKPHTIRIIGGDWKRTPLPV
CCCCCCCCCEEEEEEEEEHHHHCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCCCCE
LDLDGLRPTPDRVRETLFNWLGQDLEGRRCLDLFAGSGALGFEAASRGAASVVMVERHPR
EECCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCHHHHCCCCEEEEEEECCCH
AAQQLRALKDKLAARAIEIAEADALRIAAGLAPRSFDVVFVDPPFGDAAAFARALELAPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
LVAPGGHLYVEAGAPLDPCGHASLAGWRVVRHGKAGAVHYHLLQRENDE
CCCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MRRAVSAGSFRYQRFRIEFSRMSRSSPARPQAQRAPVAGRGKPHTIRIIGGDWKRTPLPV
CCCCCCCCCEEEEEEEEEHHHHCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCCCCE
LDLDGLRPTPDRVRETLFNWLGQDLEGRRCLDLFAGSGALGFEAASRGAASVVMVERHPR
EECCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCHHHHCCCCEEEEEEECCCH
AAQQLRALKDKLAARAIEIAEADALRIAAGLAPRSFDVVFVDPPFGDAAAFARALELAPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
LVAPGGHLYVEAGAPLDPCGHASLAGWRVVRHGKAGAVHYHLLQRENDE
CCCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]