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The map label for this gene is rpoD [H]

Identifier: 83720670

GI number: 83720670

Start: 93414

End: 94865

Strand: Reverse

Name: rpoD [H]

Synonym: BTH_I0088

Alternate gene names: 83720670

Gene position: 94865-93414 (Counterclockwise)

Preceding gene: 83720160

Following gene: 83719952

Centisome position: 2.49

GC content: 69.28

Gene sequence:

>1452_bases
ATGAAAGTCTCAGAGCGCGAAGTGCGCTACGAACAGGGCATCGACCGATTGCTCGAACTTGCCTGCGCCCGCGACGACAC
GGAATATGCCGAGCTCGCCGACGAGTTCGCGAGCGCGCCGTCCGACACACACGCTCACGCGCACGACCTCGGCGAGCCCG
AGTTCCTCGATCGCCACGCATCCGACGAAAGCGCCGGCACCGGTTCGTTCGGCGACAAGCCGACGCGAGCCGCTCACGCC
GATGCCGATTGGCACGACGGCGACGTCGTCGATGCTCAGTCGTCCGAACCCAGCGACGCCACGCCGGACGACACCGCGGT
CTCGACCGATCCGCTCGCGCTCTTCGTGCGCCGGATGCACGCGGTGCCGCTGCTGACGCACGCCCAGGAAATCGCGCTCG
CCACCGAAATCGAGGCGGGACGCGAGCAGGTGTTGCAAGCGCTCGCCGAGACGCTCGATCTTCTCGAACTCGCGTCGCTG
CCGGACGATCCGGCGCTCGGCCGCGCGCGCGCCGCGTTGCAACGCGCACGCGCCCGCGGCGACGAAGGGGCGCGCGCGTT
CGCCCGCGCGCGCAAAGCGGCGTATGCGGCGCTCGCCGCTCATGGTTGGCCGTCGGCGGAAATCGATGCGATGCGGCGTG
CAATGCGCCGGCTGCCGACGCCGCCGGAACAAGGCGATGCGGGCGCGCTGCCGGCGTTCGCGGACGGCGAGGCGCGAATC
TCCGCCGCAACCGCGAGAATGGCGGAAGCGAACCTGCGGCTCGTGCTGTCGATCGCGCGCAAATATGCGTCGCGCGGCCT
GGATCTCGCCGATCTGCTGCAGGAGGGCAACCTCGGCCTGCTGCGTGCGATCGAGAAATTCGAATATCGGCGCGGCTTCA
AGTTCTCGACGTATGCGACGTGGTGGATTCGTCAGGCGATTTCGCGCGCGATCGCGGATCGCGCTCGCACGATCCGCGTA
CCGGTTCACGTCGGCGACGAAGCGCGCCGCGTGCGCAAGGTCGCCGATCGGATCGAGCGGCGCAGCGGCGCGCGCGCATC
GCTTGCCGAACTGTCGGAGGCGACCGGGCTGTCGGCCGAGCGGCTGCGCGCGGTGCTGATGCTGCCTCGCGAGCCGCGCT
CGCTGAACGCGCCGCTCGGCGATGACGACCAGGCCGAATTCGGCGACATCGTCGAGGATGCGTCGACGCCGAGCCTGTTC
GATGCGCTCGCTCAAGGGCAAATGTGCGACGTCGTCACGTCGCTGCTGAAGACGATGTCCGCGCAGGAAGCCGACATCCT
GCGCCGGCGCTTCGGCATCGGCGGCGACGAGCCGTGGAGCTATGAGCAGATCGCGGCACAGGCGGGCGTGTCGCGCGAGC
AGATTCGCCGCATCGAGAAACGCGCGCTGCAAACGCTGCGCATCACCGCCGACGCATGCAACGCGCGCGACTTCCTCGAC
GCGCAGCCGTAA

Upstream 100 bases:

>100_bases
GTAAGGCCCATCGGCGCGGACGGGCGTCCGCGACGAGCCGACGGGCCTGGCCGACGGATTTGCTCCGTCATGACGTACCG
CGGCCCGATCCGCGTTTGCC

Downstream 100 bases:

>100_bases
CGCCCGCGCCGCTCGTCACACGCTGAAGCGGTTCAACGTATATGCGCGATACGCGGCGACGAACGCGTCGAACGATCCGG
CGTTCTTGCCTTCGAGCTCG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKVSEREVRYEQGIDRLLELACARDDTEYAELADEFASAPSDTHAHAHDLGEPEFLDRHASDESAGTGSFGDKPTRAAHA
DADWHDGDVVDAQSSEPSDATPDDTAVSTDPLALFVRRMHAVPLLTHAQEIALATEIEAGREQVLQALAETLDLLELASL
PDDPALGRARAALQRARARGDEGARAFARARKAAYAALAAHGWPSAEIDAMRRAMRRLPTPPEQGDAGALPAFADGEARI
SAATARMAEANLRLVLSIARKYASRGLDLADLLQEGNLGLLRAIEKFEYRRGFKFSTYATWWIRQAISRAIADRARTIRV
PVHVGDEARRVRKVADRIERRSGARASLAELSEATGLSAERLRAVLMLPREPRSLNAPLGDDDQAEFGDIVEDASTPSLF
DALAQGQMCDVVTSLLKTMSAQEADILRRRFGIGGDEPWSYEQIAAQAGVSREQIRRIEKRALQTLRITADACNARDFLD
AQP

Sequences:

>Translated_483_residues
MKVSEREVRYEQGIDRLLELACARDDTEYAELADEFASAPSDTHAHAHDLGEPEFLDRHASDESAGTGSFGDKPTRAAHA
DADWHDGDVVDAQSSEPSDATPDDTAVSTDPLALFVRRMHAVPLLTHAQEIALATEIEAGREQVLQALAETLDLLELASL
PDDPALGRARAALQRARARGDEGARAFARARKAAYAALAAHGWPSAEIDAMRRAMRRLPTPPEQGDAGALPAFADGEARI
SAATARMAEANLRLVLSIARKYASRGLDLADLLQEGNLGLLRAIEKFEYRRGFKFSTYATWWIRQAISRAIADRARTIRV
PVHVGDEARRVRKVADRIERRSGARASLAELSEATGLSAERLRAVLMLPREPRSLNAPLGDDDQAEFGDIVEDASTPSLF
DALAQGQMCDVVTSLLKTMSAQEADILRRRFGIGGDEPWSYEQIAAQAGVSREQIRRIEKRALQTLRITADACNARDFLD
AQP
>Mature_483_residues
MKVSEREVRYEQGIDRLLELACARDDTEYAELADEFASAPSDTHAHAHDLGEPEFLDRHASDESAGTGSFGDKPTRAAHA
DADWHDGDVVDAQSSEPSDATPDDTAVSTDPLALFVRRMHAVPLLTHAQEIALATEIEAGREQVLQALAETLDLLELASL
PDDPALGRARAALQRARARGDEGARAFARARKAAYAALAAHGWPSAEIDAMRRAMRRLPTPPEQGDAGALPAFADGEARI
SAATARMAEANLRLVLSIARKYASRGLDLADLLQEGNLGLLRAIEKFEYRRGFKFSTYATWWIRQAISRAIADRARTIRV
PVHVGDEARRVRKVADRIERRSGARASLAELSEATGLSAERLRAVLMLPREPRSLNAPLGDDDQAEFGDIVEDASTPSLF
DALAQGQMCDVVTSLLKTMSAQEADILRRRFGIGGDEPWSYEQIAAQAGVSREQIRRIEKRALQTLRITADACNARDFLD
AQP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=44.0816326530612, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=39.3805309734513, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=25.5144032921811, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 52786; Mature: 52786

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSEREVRYEQGIDRLLELACARDDTEYAELADEFASAPSDTHAHAHDLGEPEFLDRHA
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCC
SDESAGTGSFGDKPTRAAHADADWHDGDVVDAQSSEPSDATPDDTAVSTDPLALFVRRMH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AVPLLTHAQEIALATEIEAGREQVLQALAETLDLLELASLPDDPALGRARAALQRARARG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DEGARAFARARKAAYAALAAHGWPSAEIDAMRRAMRRLPTPPEQGDAGALPAFADGEARI
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
SAATARMAEANLRLVLSIARKYASRGLDLADLLQEGNLGLLRAIEKFEYRRGFKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
WWIRQAISRAIADRARTIRVPVHVGDEARRVRKVADRIERRSGARASLAELSEATGLSAE
HHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
RLRAVLMLPREPRSLNAPLGDDDQAEFGDIVEDASTPSLFDALAQGQMCDVVTSLLKTMS
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHC
AQEADILRRRFGIGGDEPWSYEQIAAQAGVSREQIRRIEKRALQTLRITADACNARDFLD
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
AQP
CCC
>Mature Secondary Structure
MKVSEREVRYEQGIDRLLELACARDDTEYAELADEFASAPSDTHAHAHDLGEPEFLDRHA
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCC
SDESAGTGSFGDKPTRAAHADADWHDGDVVDAQSSEPSDATPDDTAVSTDPLALFVRRMH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AVPLLTHAQEIALATEIEAGREQVLQALAETLDLLELASLPDDPALGRARAALQRARARG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DEGARAFARARKAAYAALAAHGWPSAEIDAMRRAMRRLPTPPEQGDAGALPAFADGEARI
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
SAATARMAEANLRLVLSIARKYASRGLDLADLLQEGNLGLLRAIEKFEYRRGFKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
WWIRQAISRAIADRARTIRVPVHVGDEARRVRKVADRIERRSGARASLAELSEATGLSAE
HHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
RLRAVLMLPREPRSLNAPLGDDDQAEFGDIVEDASTPSLFDALAQGQMCDVVTSLLKTMS
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHC
AQEADILRRRFGIGGDEPWSYEQIAAQAGVSREQIRRIEKRALQTLRITADACNARDFLD
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
AQP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]