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The map label for this gene is purU [H]

Identifier: 83720563

GI number: 83720563

Start: 547950

End: 548831

Strand: Direct

Name: purU [H]

Synonym: BTH_I0496

Alternate gene names: 83720563

Gene position: 547950-548831 (Clockwise)

Preceding gene: 83721295

Following gene: 83719859

Centisome position: 14.38

GC content: 65.65

Gene sequence:

>882_bases
ATGTCGGCCGATCACAGCTTTATCCTGAAACTGTCGTGCCCGGACCGGCGCGGCATCGTTCACGCGGTCTCCGGCTTTTT
GCTAGAGCGCGGCAGCAACATCCTCGATTCGGCGCAGTTCGGCGACAGCCGCACCGGCGAATTCTTCATGCGCGTGCATT
TCGAGCAGGCGGGCGCCGCCGATGCCGCGGCCGCGCTCGACGGCCTGCGCGCGGAGTTCGCGCCGCTCGCCGAGCAGTTC
GCGATGCGCTGGGAACTGCACGACGCGTCGGTGAAGCCGCGCGTCGTGATCCTCGTGTCGAAGATCGGCCATTGCCTGAA
CGACCTGCTGTTCCGCTACCGCACGGGCCAGTTGCCGATCGAGATCCCGGCGATCGTGTCGAATCACAAGGACTTCTATC
AGCTCGCCGCGAGCTACGACATTCCGTTCCACCACTTCCCGCTCGCCGCGGGCGCGTCGGCCGACGCGAAGGCCGCGCAG
GAAGCGCGCGTGCTCGAGGTGATCGACGAGCATGCGGCCGATCTCGTCGTGCTCGCGCGCTACATGCAGATCCTGTCGCC
GAACATGTGCGAGCGGCTCGCGGGGCGCGCGATCAACATCCATCACTCGTTCCTGCCGAGCTTCAAGGGTGCGAAGCCGT
ACTATCAGGCGTTCGACCGCGGCGTGAAGCTGATCGGCGCGACCGCGCACTATGTGACGACCGACCTCGACGAAGGCCCG
ATCATCGAGCAGGAAGTCGAGCGCGTCGATCACAGCATGACGCCGGACGAGCTGACGGCCGTGGGCCGCGATGTCGAATG
CGTGACGCTCGCGCGTGCGGTCAAGTGGCACGTCGAGCACAGGATCGTGCTGAACGGCACGAAGACGGTGGTGTTTCGCT
AA

Upstream 100 bases:

>100_bases
CGCGCGGGGAATCGACGCGCTGTTCGTGCCGCCGCCGCTTGGCTGAGCGCGACGCGAACGCATTGCATTGTTCGGAAGAT
TTGAAAAGGGAGTAGCGGTC

Downstream 100 bases:

>100_bases
GCCGGCCGCGCGCGTTTTCCGTCGGCTGGCCGGGCCGGCTCGCGACGCGCATGGCTGGCGGCTGGCCGCGCATGCGGCCG
CGCGACGGCAAAAGGAAGGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAADAAAALDGLRAEFAPLAEQF
AMRWELHDASVKPRVVILVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHFPLAAGASADAKAAQ
EARVLEVIDEHAADLVVLARYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR

Sequences:

>Translated_293_residues
MSADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAADAAAALDGLRAEFAPLAEQF
AMRWELHDASVKPRVVILVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHFPLAAGASADAKAAQ
EARVLEVIDEHAADLVVLARYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
>Mature_292_residues
SADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAADAAAALDGLRAEFAPLAEQFA
MRWELHDASVKPRVVILVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHFPLAAGASADAKAAQE
ARVLEVIDEHAADLVVLARYMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPI
IEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=174, Percent_Identity=33.9080459770115, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869995, Length=174, Percent_Identity=33.9080459770115, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869993, Length=174, Percent_Identity=33.9080459770115, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=39.2982456140351, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=30.7291666666667, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=234, Percent_Identity=29.9145299145299, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32497; Mature: 32366

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEEHHCCCH
DAAAALDGLRAEFAPLAEQFAMRWELHDASVKPRVVILVSKIGHCLNDLLFRYRTGQLPI
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCE
EIPAIVSNHKDFYQLAASYDIPFHHFPLAAGASADAKAAQEARVLEVIDEHAADLVVLAR
EEHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
HHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCC
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
CCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEC
>Mature Secondary Structure 
SADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEEHHCCCH
DAAAALDGLRAEFAPLAEQFAMRWELHDASVKPRVVILVSKIGHCLNDLLFRYRTGQLPI
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCE
EIPAIVSNHKDFYQLAASYDIPFHHFPLAAGASADAKAAQEARVLEVIDEHAADLVVLAR
EEHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YMQILSPNMCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
HHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCC
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
CCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]