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The map label for this gene is gloA [H]

Identifier: 83720549

GI number: 83720549

Start: 662089

End: 662478

Strand: Direct

Name: gloA [H]

Synonym: BTH_I0580

Alternate gene names: 83720549

Gene position: 662089-662478 (Clockwise)

Preceding gene: 83719203

Following gene: 83721494

Centisome position: 17.38

GC content: 61.28

Gene sequence:

>390_bases
ATGCGTTTGCTTCACACGATGCTGCGAGTCGGCGATCTCGACCGCTCGATCAAGTTCTACACCGAACTGCTCGGCATGAA
ACTGCTGCGCCGCCAAGACTATCCGGACGGCAAGTTCACGCTCGCGTTCGTCGGCTACGGCGACGAGCAGGACCACACGG
TCATCGAGCTCACCCACAACTGGGACACGAAATCGTACGAGCTCGGCACGGGCTTCGGCCATCTCGCCGTCGAAGTCGAG
GACGCGTACAAGGCGTGCGAGCAGATCAAGGCGCAAGGCGGCAAGGTCACGCGCGAAGCGGGCCCGATGAAGCACGGCAC
GACGGTGATCGCGTTCGTCGAAGATCCGGACGGCTACAAGATCGAGTTCATCCAGCGCAAGACTCACTGA

Upstream 100 bases:

>100_bases
CATCGTCCCTGCTGCGCGGATGGGCGATGCGGCGCCCGGCGAGAATCGCGCGGGTCTGGTACATTCGCACGTTCAAGCAA
GTCCCCACGGAGTACCCATC

Downstream 100 bases:

>100_bases
GCGCTGTCCCGCCGCGGCGCCGCCGTGTCTCGGCGCGCGATGCGCTTTAAGCGTGCGCGCGCCACGTCTTTTTCTCCACC
AATCCATTCTTCCCGGGCTG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDEQDHTVIELTHNWDTKSYELGTGFGHLAVEVE
DAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRKTH

Sequences:

>Translated_129_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDEQDHTVIELTHNWDTKSYELGTGFGHLAVEVE
DAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRKTH
>Mature_129_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDEQDHTVIELTHNWDTKSYELGTGFGHLAVEVE
DAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRKTH

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=149, Percent_Identity=38.255033557047, Blast_Score=97, Evalue=4e-21,
Organism=Homo sapiens, GI217330598, Length=138, Percent_Identity=28.2608695652174, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=67.7165354330709, Blast_Score=185, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=37.410071942446, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129743, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=28.9855072463768, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14736; Mature: 14736

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDEQDHTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTKSYELGTGFGHLAVEVEDAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCEEECCCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQRKTH
EEEEEECCC
>Mature Secondary Structure
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDEQDHTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTKSYELGTGFGHLAVEVEDAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCEEECCCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQRKTH
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]