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The map label for this gene is ppnK [H]

Identifier: 83720497

GI number: 83720497

Start: 1461278

End: 1462177

Strand: Reverse

Name: ppnK [H]

Synonym: BTH_I1301

Alternate gene names: 83720497

Gene position: 1462177-1461278 (Counterclockwise)

Preceding gene: 83719577

Following gene: 83720672

Centisome position: 38.39

GC content: 67.22

Gene sequence:

>900_bases
ATGAAAATCGGCCACCAATTCCATACCGTCGCGCTCGTCGGGCGCAGCAACACGCCCGGCATCGCCGAGCCGCTCGCGTC
GCTCGCCGCGTGCATCGCGAAGCGCGGCTTCGAAGTCGTGTTCGAGGCGGACACCGCGCAGGCGCTCGGCAGCACCGGCT
ACCCGGCGCTCACGCCCGCCGAGATCGGCGCGCGCGCCGACGTCGCGGTCGTGCTGGGCGGCGACGGCACGATGCTCGGC
ATCGGCCGCCAGCTCGCGCCGTACAAGACGCCGCTCATCGGCATCAACCACGGCCGGCTCGGCTTCATCACCGACATCCC
GGCGTCCGACATGCAGGAAGTCGTGCCGATGATGCTCGCGGGCAGCTACGAGCGCGAGGAGCGCACGCTGCTCGAGGCGC
GGATCGTGCGCAACAACGAGCCGATCTACCACGCGCTCGCGTTCAACGACGTCGTCGTGAACCGCAGCGGCTTCTCCGGG
ATGGCCGAGCTGCGCGTGTCGGTCGACGGCCGATTCATGTACAACCAGCGCTCGGACGGCCTCATCGTCGCGACGCCGAC
GGGCTCGACCGCATACGCGCTGTCGTCGCAGGGGCCGATCCTGCATCCGCAGCTGCAGGGCATCGTGCTCGTGCCGATCG
CGCCGCACGCGCTGTCGAACCGGCCGATCGTGCTGCCGGACGATTCGAAGATCGCGATCCAGATCATCGGCGGGCGCGAC
GTGAACGTGAACTTCGACATGCAGTCGTTCACCGCGCTCGAGTTGAACGACACGATCGAAGTGCGCCGCTCGAAGCACAC
GGTGCCGTTCCTGCACCCGGTCGGCTACAGCTATTACGCGACGCTGCGCAAGAAACTGCACTGGAACGAACACCCGTCGA
ACGAAGAAGACGACGAATAA

Upstream 100 bases:

>100_bases
CCGCGAGCGCCGCTTGCGCGCCGCCCGTCCCGCGCCCGTTTTGCCGGCCGGCCGCCGGCGGGCGGTTCTTTTCGTCATCG
AGCTATGGTGTAATGCCGGC

Downstream 100 bases:

>100_bases
ATCCCGACGCCCCCCTTCCGACCGACACCCATGCTCCGCCACCTCTCGATTCGCGATTTCGTCATCGTCGCCGCACTCGA
CCTGGAATTCGACAGCGGCT

Product: NAD(+)/NADH kinase family protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKIGHQFHTVALVGRSNTPGIAEPLASLAACIAKRGFEVVFEADTAQALGSTGYPALTPAEIGARADVAVVLGGDGTMLG
IGRQLAPYKTPLIGINHGRLGFITDIPASDMQEVVPMMLAGSYEREERTLLEARIVRNNEPIYHALAFNDVVVNRSGFSG
MAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHALSNRPIVLPDDSKIAIQIIGGRD
VNVNFDMQSFTALELNDTIEVRRSKHTVPFLHPVGYSYYATLRKKLHWNEHPSNEEDDE

Sequences:

>Translated_299_residues
MKIGHQFHTVALVGRSNTPGIAEPLASLAACIAKRGFEVVFEADTAQALGSTGYPALTPAEIGARADVAVVLGGDGTMLG
IGRQLAPYKTPLIGINHGRLGFITDIPASDMQEVVPMMLAGSYEREERTLLEARIVRNNEPIYHALAFNDVVVNRSGFSG
MAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHALSNRPIVLPDDSKIAIQIIGGRD
VNVNFDMQSFTALELNDTIEVRRSKHTVPFLHPVGYSYYATLRKKLHWNEHPSNEEDDE
>Mature_299_residues
MKIGHQFHTVALVGRSNTPGIAEPLASLAACIAKRGFEVVFEADTAQALGSTGYPALTPAEIGARADVAVVLGGDGTMLG
IGRQLAPYKTPLIGINHGRLGFITDIPASDMQEVVPMMLAGSYEREERTLLEARIVRNNEPIYHALAFNDVVVNRSGFSG
MAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHALSNRPIVLPDDSKIAIQIIGGRD
VNVNFDMQSFTALELNDTIEVRRSKHTVPFLHPVGYSYYATLRKKLHWNEHPSNEEDDE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=260, Percent_Identity=28.4615384615385, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1788968, Length=289, Percent_Identity=39.1003460207612, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=29.7520661157025, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322509, Length=242, Percent_Identity=30.5785123966942, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325068, Length=182, Percent_Identity=34.6153846153846, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573828, Length=261, Percent_Identity=30.2681992337165, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161077047, Length=261, Percent_Identity=30.2681992337165, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28573830, Length=261, Percent_Identity=30.2681992337165, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28573826, Length=261, Percent_Identity=30.2681992337165, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28573832, Length=261, Percent_Identity=30.2681992337165, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI20129957, Length=286, Percent_Identity=28.3216783216783, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24653422, Length=286, Percent_Identity=28.3216783216783, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281363323, Length=286, Percent_Identity=28.3216783216783, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281363321, Length=286, Percent_Identity=28.3216783216783, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24653424, Length=286, Percent_Identity=28.3216783216783, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 32449; Mature: 32449

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGHQFHTVALVGRSNTPGIAEPLASLAACIAKRGFEVVFEADTAQALGSTGYPALTPA
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCHH
EIGARADVAVVLGGDGTMLGIGRQLAPYKTPLIGINHGRLGFITDIPASDMQEVVPMMLA
HCCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHC
GSYEREERTLLEARIVRNNEPIYHALAFNDVVVNRSGFSGMAELRVSVDGRFMYNQRSDG
CCCCHHHHHHHHHHEECCCCCEEEEEEECEEEEECCCCCCEEEEEEEECCEEEECCCCCC
LIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHALSNRPIVLPDDSKIAIQIIGGRD
EEEECCCCCEEEEECCCCCEECCCCCCEEEEEECCHHCCCCCEEEECCCEEEEEEECCEE
VNVNFDMQSFTALELNDTIEVRRSKHTVPFLHPVGYSYYATLRKKLHWNEHPSNEEDDE
EEEEECCCCEEEEEECCCEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKIGHQFHTVALVGRSNTPGIAEPLASLAACIAKRGFEVVFEADTAQALGSTGYPALTPA
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCHH
EIGARADVAVVLGGDGTMLGIGRQLAPYKTPLIGINHGRLGFITDIPASDMQEVVPMMLA
HCCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHC
GSYEREERTLLEARIVRNNEPIYHALAFNDVVVNRSGFSGMAELRVSVDGRFMYNQRSDG
CCCCHHHHHHHHHHEECCCCCEEEEEEECEEEEECCCCCCEEEEEEEECCEEEECCCCCC
LIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHALSNRPIVLPDDSKIAIQIIGGRD
EEEECCCCCEEEEECCCCCEECCCCCCEEEEEECCHHCCCCCEEEECCCEEEEEEECCEE
VNVNFDMQSFTALELNDTIEVRRSKHTVPFLHPVGYSYYATLRKKLHWNEHPSNEEDDE
EEEEECCCCEEEEEECCCEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA