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The map label for this gene is psdht [H]

Identifier: 83720477

GI number: 83720477

Start: 2360720

End: 2361751

Strand: Reverse

Name: psdht [H]

Synonym: BTH_I2085

Alternate gene names: 83720477

Gene position: 2361751-2360720 (Counterclockwise)

Preceding gene: 161723180

Following gene: 83721374

Centisome position: 62.0

GC content: 68.22

Gene sequence:

>1032_bases
ATGTCGACCTCACCCCACCGCCCCGCTCATCTGACGATCAACGGCGAGCCGATTCCGACGCTCGACGAAATCGCCGCGCA
GCACTTCGCGCTCACGCCGTGGGTCACGCGTACGCCGGTGTTCGAGCGCGCCGATCTGCCATCGCTCGACAACACGCACG
TGAACTTCAAGTTCGAGCTGCTGCAGGCGAGCGGCAGCTTCAAGGCGCGCGGCGCGTTCACGAACCTGCTCGCGCTCGGC
GAGGACAGAAAGCGCGCGGGCGTCACCTGCGTGTCGGCGGGCAATCATGCGGCGGCCGTCGCTTATGCGGCGATGCGGCT
CGGCACGAGCGCGAAGGTCGTGATGTTCGACACCGCGAGCCCGGCGCGCGTCGAGCTCTGCCACCAGTACCGCGCGGAGG
TCGTGATGGCGTCGAGCCCCGCCGACGCGTTCGAGATCGTCCGGCGAATCGAGGCCGAGGAAGGCCGCTTCTTCGTGCAT
CCGTTCAACGGCTATCACACGATTCTCGGCACCGCGACGCTCGGCTACGAATGGGCGACGCAGACGCCCGACCTCGATGC
GGTGATCGTGCCGATCGGCGGCGGCGGCCTCGCCGCGGGCACCTCGACCGCGCTGCGGCTTGCGAATCCGCGCGTGCACG
TGTACGGCGTCGAGCCGGAAGGTGCGGATGTGATGAGCCGCAGCTTCGCCGCGAATCGCCCGGTCCGCATGGGCGCGATG
CATACGATCGCCGATTCGCTGATGGCGCCGCACACCGAGGAGTACAGCTACGAGCTGTGCCGCCGCCATGTCGACCGGAT
CGTCACCGTCTCGGACGACGCGCTGCGAGCGGCGATGCTCTATTTGTTCACGCATCTGAAAGTGGCGGTCGAGCCCGCGT
GCGCGGCCGCGACGGCCGCCCTCCTCGGGCCGTTGCGCGAGTCGCTGCAAGGCAAGCGTGTTGGCGTGCTGCTGTCGGGC
ACCAACATCGATTCGACGGCGCTCGCCACGCATCTCGAGCATGCTCGGCTGCATCGCATGTCGTGGCCGTGA

Upstream 100 bases:

>100_bases
CGCAAGCTCGTGCAGGGCATGGCGCTCGCGTGAGCCCGCGCGCCGCGGCGAACTTCGTTTCGGCCAGCATCCGGCCTCCG
TCATCGATACGCGCACGCAC

Downstream 100 bases:

>100_bases
CGCCGGTTATCCCCAGTTCGTGTTGAAGAGCATGTTGAAAAGCCGCGAACAAGCGCGCTAACGCGTTGATCGCGCGGCGT
TTTCCGGGAGCGCCGCGAAA

Product: threonine dehydratase catabolic

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSTSPHRPAHLTINGEPIPTLDEIAAQHFALTPWVTRTPVFERADLPSLDNTHVNFKFELLQASGSFKARGAFTNLLALG
EDRKRAGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTASPARVELCHQYRAEVVMASSPADAFEIVRRIEAEEGRFFVH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADVMSRSFAANRPVRMGAM
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFTHLKVAVEPACAAATAALLGPLRESLQGKRVGVLLSG
TNIDSTALATHLEHARLHRMSWP

Sequences:

>Translated_343_residues
MSTSPHRPAHLTINGEPIPTLDEIAAQHFALTPWVTRTPVFERADLPSLDNTHVNFKFELLQASGSFKARGAFTNLLALG
EDRKRAGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTASPARVELCHQYRAEVVMASSPADAFEIVRRIEAEEGRFFVH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADVMSRSFAANRPVRMGAM
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFTHLKVAVEPACAAATAALLGPLRESLQGKRVGVLLSG
TNIDSTALATHLEHARLHRMSWP
>Mature_342_residues
STSPHRPAHLTINGEPIPTLDEIAAQHFALTPWVTRTPVFERADLPSLDNTHVNFKFELLQASGSFKARGAFTNLLALGE
DRKRAGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTASPARVELCHQYRAEVVMASSPADAFEIVRRIEAEEGRFFVHP
FNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADVMSRSFAANRPVRMGAMH
TIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFTHLKVAVEPACAAATAALLGPLRESLQGKRVGVLLSGT
NIDSTALATHLEHARLHRMSWP

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=321, Percent_Identity=30.2180685358255, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=38.7453874538745, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790207, Length=306, Percent_Identity=32.3529411764706, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71991565, Length=312, Percent_Identity=33.974358974359, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17537387, Length=317, Percent_Identity=33.1230283911672, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=27.9365079365079, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322631, Length=315, Percent_Identity=34.9206349206349, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320930, Length=271, Percent_Identity=29.8892988929889, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6319788, Length=285, Percent_Identity=27.3684210526316, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322024, Length=151, Percent_Identity=29.1390728476821, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355833, Length=333, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24645328, Length=214, Percent_Identity=34.1121495327103, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 37058; Mature: 36926

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSPHRPAHLTINGEPIPTLDEIAAQHFALTPWVTRTPVFERADLPSLDNTHVNFKFEL
CCCCCCCCEEEEECCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEE
LQASGSFKARGAFTNLLALGEDRKRAGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTAS
EECCCCCEECHHHHHHHHCCCCCHHCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PARVELCHQYRAEVVMASSPADAFEIVRRIEAEEGRFFVHPFNGYHTILGTATLGYEWAT
CHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADVMSRSFAANRPVRMGAM
CCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHH
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFTHLKVAVEPACAAATAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LLGPLRESLQGKRVGVLLSGTNIDSTALATHLEHARLHRMSWP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STSPHRPAHLTINGEPIPTLDEIAAQHFALTPWVTRTPVFERADLPSLDNTHVNFKFEL
CCCCCCCEEEEECCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEE
LQASGSFKARGAFTNLLALGEDRKRAGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTAS
EECCCCCEECHHHHHHHHCCCCCHHCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PARVELCHQYRAEVVMASSPADAFEIVRRIEAEEGRFFVHPFNGYHTILGTATLGYEWAT
CHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADVMSRSFAANRPVRMGAM
CCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHH
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFTHLKVAVEPACAAATAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LLGPLRESLQGKRVGVLLSGTNIDSTALATHLEHARLHRMSWP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]