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The map label for this gene is acrA [H]

Identifier: 83720471

GI number: 83720471

Start: 2799288

End: 2800487

Strand: Reverse

Name: acrA [H]

Synonym: BTH_I2445

Alternate gene names: 83720471

Gene position: 2800487-2799288 (Counterclockwise)

Preceding gene: 83721079

Following gene: 83720423

Centisome position: 73.52

GC content: 74.0

Gene sequence:

>1200_bases
ATGAAATACGAATGGGCACGCATGCGCCGCAGGTGGGCGGCGCTCGCGGTCGCGGCGTTCGTCGCGGCCGGCTGCGGCAA
GCGCGAAAGCGCCCACGAGGCGGCCGCGTCGCGCGAGGCGAGCGTCGTCACGGTGAAGAAGACGTCGGTGCCGCTGTCGG
TCGAGCTGCCGGGCCGGCTCGACGCATACCGGCAGGCCGAGGTGCGCGCGCGAGTGGCGGGCATCGTGACCGCGCGGACC
TACGAGGAAGGGCAGGAGGTCAAGCGCGGCGCGGTGCTGTTCAGGATCGATCCCGCGCCGTTCAAGGCGGCGCGCGACGC
GGCCGCGGGCGCGCTCGAGAAGGCGCAGGCCGCGCATCTCGCGGCGCTCGACAAGCGCCGCCGCTACGACGAGCTCGTGC
GCGACCGCGCGGTCAGCGAGCGCGACCACACCGAGGCGCTCGCCGACGAGCGGCAGGCGAAGGCGGCCGTCGCGTCGGCG
CGCGCCGAGCTCGCGCGCGCGCAACTGCAGCTCGACTACGCGACCGTCACGTCGCCGATCGACGGCCGCGCGCGCCGCGC
GCTCGTGACGGAGGGCGCGCTCGTCGGCCAGGATCAGGCGACGCCGCTCACGACCGTCGAGCAGCTCGATCCGATCTACG
TGAACTTCTCGCAGCCGGCCGCCGACGTCGAATCGCTGCGGCGCGCGGTGAAGAGCGGGCGCGCGGCGGGCATCGCGCAG
CAGGACGTCGAGGTGACGCTCGTGCGTCCGGACGGCAGCACTTACGCGCGCAAGGGCAAGCTGCTGTTCGCGGATCTCGC
GGTCGATCCGTCCACCGACACGGTCGCGATGCGCGCGCTCTTTCCGAATCCGGAGCGCGAACTGCTGCCGGGCGCGTACG
TGCGGATCGCGCTCGATCGCGCGGTCGCGCGCGACGCGATCCTCGTGCCGCGCGACGCGCTCCTGCGCACGGCCGACAGC
GCGACCGTCAAGGTCGTCGGCGAGAACGGAAAGATACGCGACGTGACGGTCGAGGCCACGCAGATGAAAGGCCGCGACTG
GATCGTCACGCGCGGGCTCGCGGGCGGCGAGCGCGTCGTCGTCGTCGACGCCGCGCAATTCGATGCGGGCGCGACCGTGA
AGGCGGTCGAGCGCGGCGCCGCCGCGCAGCCGGCCTCCGGCGCCGCCGCGGCTGCCGCGCCCGACAAGCGCCAAACCTGA

Upstream 100 bases:

>100_bases
ATATGTCGGACGACATTATTTAATCAATCGTGACTGATTATAATCGATCGCCCCGACCGGCCCCAAATTCGTCCCGGTCG
AGCGACTTGCGAGGAAAACG

Downstream 100 bases:

>100_bases
CAGGCACAACTCGATCATGGCTCGTTTCTTCATCGATCGCCCGGTGTTCGCATGGGTGATCTCCTTATTCATCATGCTGG
GCGGCATCTTCGCGATCCGC

Product: periplasmic multidrug efflux lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKYEWARMRRRWAALAVAAFVAAGCGKRESAHEAAASREASVVTVKKTSVPLSVELPGRLDAYRQAEVRARVAGIVTART
YEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHLAALDKRRRYDELVRDRAVSERDHTEALADERQAKAAVASA
RAELARAQLQLDYATVTSPIDGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVESLRRAVKSGRAAGIAQ
QDVEVTLVRPDGSTYARKGKLLFADLAVDPSTDTVAMRALFPNPERELLPGAYVRIALDRAVARDAILVPRDALLRTADS
ATVKVVGENGKIRDVTVEATQMKGRDWIVTRGLAGGERVVVVDAAQFDAGATVKAVERGAAAQPASGAAAAAAPDKRQT

Sequences:

>Translated_399_residues
MKYEWARMRRRWAALAVAAFVAAGCGKRESAHEAAASREASVVTVKKTSVPLSVELPGRLDAYRQAEVRARVAGIVTART
YEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHLAALDKRRRYDELVRDRAVSERDHTEALADERQAKAAVASA
RAELARAQLQLDYATVTSPIDGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVESLRRAVKSGRAAGIAQ
QDVEVTLVRPDGSTYARKGKLLFADLAVDPSTDTVAMRALFPNPERELLPGAYVRIALDRAVARDAILVPRDALLRTADS
ATVKVVGENGKIRDVTVEATQMKGRDWIVTRGLAGGERVVVVDAAQFDAGATVKAVERGAAAQPASGAAAAAAPDKRQT
>Mature_399_residues
MKYEWARMRRRWAALAVAAFVAAGCGKRESAHEAAASREASVVTVKKTSVPLSVELPGRLDAYRQAEVRARVAGIVTART
YEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHLAALDKRRRYDELVRDRAVSERDHTEALADERQAKAAVASA
RAELARAQLQLDYATVTSPIDGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVESLRRAVKSGRAAGIAQ
QDVEVTLVRPDGSTYARKGKLLFADLAVDPSTDTVAMRALFPNPERELLPGAYVRIALDRAVARDAILVPRDALLRTADS
ATVKVVGENGKIRDVTVEATQMKGRDWIVTRGLAGGERVVVVDAAQFDAGATVKAVERGAAAQPASGAAAAAAPDKRQT

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=399, Percent_Identity=39.0977443609023, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=40.4255319148936, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1789929, Length=373, Percent_Identity=35.9249329758713, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082036, Length=343, Percent_Identity=29.1545189504373, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42661; Mature: 42661

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYEWARMRRRWAALAVAAFVAAGCGKRESAHEAAASREASVVTVKKTSVPLSVELPGRL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCEEEECCCCC
DAYRQAEVRARVAGIVTARTYEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHL
CHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AALDKRRRYDELVRDRAVSERDHTEALADERQAKAAVASARAELARAQLQLDYATVTSPI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCC
DGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVESLRRAVKSGRAAGIAQ
CCHHHHHEEECCCEECCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
QDVEVTLVRPDGSTYARKGKLLFADLAVDPSTDTVAMRALFPNPERELLPGAYVRIALDR
CCCEEEEECCCCCCHHHCCCEEEEEEECCCCCCCEEEEEECCCCCHHHCCCCEEEEEEHH
AVARDAILVPRDALLRTADSATVKVVGENGKIRDVTVEATQMKGRDWIVTRGLAGGERVV
HHHCCEEECCHHHHHHCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEECCCCCCEEE
VVDAAQFDAGATVKAVERGAAAQPASGAAAAAAPDKRQT
EEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKYEWARMRRRWAALAVAAFVAAGCGKRESAHEAAASREASVVTVKKTSVPLSVELPGRL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCEEEECCCCC
DAYRQAEVRARVAGIVTARTYEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHL
CHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AALDKRRRYDELVRDRAVSERDHTEALADERQAKAAVASARAELARAQLQLDYATVTSPI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCC
DGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVESLRRAVKSGRAAGIAQ
CCHHHHHEEECCCEECCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
QDVEVTLVRPDGSTYARKGKLLFADLAVDPSTDTVAMRALFPNPERELLPGAYVRIALDR
CCCEEEEECCCCCCHHHCCCEEEEEEECCCCCCCEEEEEECCCCCHHHCCCCEEEEEEHH
AVARDAILVPRDALLRTADSATVKVVGENGKIRDVTVEATQMKGRDWIVTRGLAGGERVV
HHHCCEEECCHHHHHHCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEECCCCCCEEE
VVDAAQFDAGATVKAVERGAAAQPASGAAAAAAPDKRQT
EEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]