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The map label for this gene is intF [H]

Identifier: 83720438

GI number: 83720438

Start: 100027

End: 101376

Strand: Direct

Name: intF [H]

Synonym: BTH_I0094

Alternate gene names: 83720438

Gene position: 100027-101376 (Clockwise)

Preceding gene: 83719741

Following gene: 83721017

Centisome position: 2.63

GC content: 61.63

Gene sequence:

>1350_bases
ATGGTGAGCAAGCAGAACTTCACGGCGGCGCGGGTCGACGCATTCCAATGCGAGCCAGGCAAGCAGCAGACGATCTACTG
GGACGCAAAGACACCTGGCTTCGGGTTGCGCGTTACGGCGGCGGGTGCAAGAGCCTTCATATTTGAATCCAGACTCTTCG
GCAAGACGGTTAGGTTGACCATCGGAGACGTTCGGGCGTGGGACCTGGGCCGAGCGCGCACCGAGGCTGCGAGGCTCAAG
ACGCTGATTGACGACGGCAAGGATCCGCGGGAGGTGCGAGCAGAGCAGCAGGCCGAACATGAGGCACGCCGCGCGGAAGC
TCGGCGGCAGGACGCGTTGTTTGGTGTCGTATGGGACGAGTACACGGAGAGCCGGAAGGCGTTCTGGGGTGAGCGCCACT
ATCGCGATCACTTGCAGCATGCAGACGAAGGAGGGCAGCCAAAGAAGCGCGGCAAGGGCGTAACGCAGCCCGGTCCGTTG
GCGGCACTTCGTCCTCTGAAGCTGTCTGAATTGACCAGTGAGCGGATCGCCGACTGGCTCACCGCCGAGTCGCGAGAGCG
GCCGACGATGTCGGCGCTGTCATTTCGCCTGCTGCGCGGATTCATCCGCTGGGCCGATGACACATCGGCTTACAAGGGCA
TTGTTCCGATAGATGCCTACAAGGCCCGCGACGTTCGCGATGTGGTCCCGCGCGTAAAGGCCAAGGATGGCGATTCATTG
CAGCGGGAGCAATTAGGAGTGTGGTTCCGCGAGGTCCGCAAGATTGCGAATCCGGTCCAAAGCGCGTATCTGCAGGGGCT
GTTGCTTACCGGTGCGCGTCGTGAGGAGTGGGCGGGACTGCGGTGGGAGGATATTGATTTCCGCTGGCGAAGCGTCGTGC
TCGATGACAAGGTCGAGGGGACTGGTGGCCGGACAATTCCTCTTACTCCATACCTCGCGACGCTGCTGATGAACTTGAAG
CGCCTGAACGAGACGCCGCCGGACCGGCGACAACTCGCGCGCCTCGCCGAGCGTAGAGAGACCTGGGTGCCATCCGAATG
GGTGTTCGCGAGCAAGACCTCGGAAGACGGGAAGATCGCTGAACCCCGGATTGCGCACAACAAGGCGCTGGCGGCGGCCG
AACTCCCGCACGTCACATTGCATGGTCTTCGTCGCTCTTTCGGCACGCTGTCGGAATGGGTTGAAGTACCCGTGGGCGTG
GTAGCGCAAATTCAGGGGCACAAGCCGTCGGCGATCGCCGAGAAACACTATCGTCGTCGTCCCCTTGATCTGCTACGAAT
GTGGCACGACAAAGTCGAGTCTTGGATGTTGGAGCAAGCGGGAGTCCAGTTTCAGGTTGATCCGGCTTGA

Upstream 100 bases:

>100_bases
CTTGTTGGGAAAGGATTTTAGGCCGAATCCACGGATTTTCATGTTGACCGATATGGTCCAATTTCCCTAGTATTTGCCTA
CATGGTGCCTACACGGGGAA

Downstream 100 bases:

>100_bases
AATACGAGGGGGCGATCAGCCGCTCCGCTTTCCGTTTCTAGTTCGCTCCACGCTAGAATCTTCAGTCGATGGCGACAATT
GATCGCCGCAGATTGTCACA

Product: Phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MVSKQNFTAARVDAFQCEPGKQQTIYWDAKTPGFGLRVTAAGARAFIFESRLFGKTVRLTIGDVRAWDLGRARTEAARLK
TLIDDGKDPREVRAEQQAEHEARRAEARRQDALFGVVWDEYTESRKAFWGERHYRDHLQHADEGGQPKKRGKGVTQPGPL
AALRPLKLSELTSERIADWLTAESRERPTMSALSFRLLRGFIRWADDTSAYKGIVPIDAYKARDVRDVVPRVKAKDGDSL
QREQLGVWFREVRKIANPVQSAYLQGLLLTGARREEWAGLRWEDIDFRWRSVVLDDKVEGTGGRTIPLTPYLATLLMNLK
RLNETPPDRRQLARLAERRETWVPSEWVFASKTSEDGKIAEPRIAHNKALAAAELPHVTLHGLRRSFGTLSEWVEVPVGV
VAQIQGHKPSAIAEKHYRRRPLDLLRMWHDKVESWMLEQAGVQFQVDPA

Sequences:

>Translated_449_residues
MVSKQNFTAARVDAFQCEPGKQQTIYWDAKTPGFGLRVTAAGARAFIFESRLFGKTVRLTIGDVRAWDLGRARTEAARLK
TLIDDGKDPREVRAEQQAEHEARRAEARRQDALFGVVWDEYTESRKAFWGERHYRDHLQHADEGGQPKKRGKGVTQPGPL
AALRPLKLSELTSERIADWLTAESRERPTMSALSFRLLRGFIRWADDTSAYKGIVPIDAYKARDVRDVVPRVKAKDGDSL
QREQLGVWFREVRKIANPVQSAYLQGLLLTGARREEWAGLRWEDIDFRWRSVVLDDKVEGTGGRTIPLTPYLATLLMNLK
RLNETPPDRRQLARLAERRETWVPSEWVFASKTSEDGKIAEPRIAHNKALAAAELPHVTLHGLRRSFGTLSEWVEVPVGV
VAQIQGHKPSAIAEKHYRRRPLDLLRMWHDKVESWMLEQAGVQFQVDPA
>Mature_449_residues
MVSKQNFTAARVDAFQCEPGKQQTIYWDAKTPGFGLRVTAAGARAFIFESRLFGKTVRLTIGDVRAWDLGRARTEAARLK
TLIDDGKDPREVRAEQQAEHEARRAEARRQDALFGVVWDEYTESRKAFWGERHYRDHLQHADEGGQPKKRGKGVTQPGPL
AALRPLKLSELTSERIADWLTAESRERPTMSALSFRLLRGFIRWADDTSAYKGIVPIDAYKARDVRDVVPRVKAKDGDSL
QREQLGVWFREVRKIANPVQSAYLQGLLLTGARREEWAGLRWEDIDFRWRSVVLDDKVEGTGGRTIPLTPYLATLLMNLK
RLNETPPDRRQLARLAERRETWVPSEWVFASKTSEDGKIAEPRIAHNKALAAAELPHVTLHGLRRSFGTLSEWVEVPVGV
VAQIQGHKPSAIAEKHYRRRPLDLLRMWHDKVESWMLEQAGVQFQVDPA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI2367104, Length=453, Percent_Identity=50.3311258278146, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 51235; Mature: 51235

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKQNFTAARVDAFQCEPGKQQTIYWDAKTPGFGLRVTAAGARAFIFESRLFGKTVRLT
CCCCCCCCHHHCEEEECCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCEEEEE
IGDVRAWDLGRARTEAARLKTLIDDGKDPREVRAEQQAEHEARRAEARRQDALFGVVWDE
ECCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHH
YTESRKAFWGERHYRDHLQHADEGGQPKKRGKGVTQPGPLAALRPLKLSELTSERIADWL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
TAESRERPTMSALSFRLLRGFIRWADDTSAYKGIVPIDAYKARDVRDVVPRVKAKDGDSL
HHCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHCCCHHHHHHHCCCCCCCHH
QREQLGVWFREVRKIANPVQSAYLQGLLLTGARREEWAGLRWEDIDFRWRSVVLDDKVEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEECCCCEEEEEEEECCCCC
TGGRTIPLTPYLATLLMNLKRLNETPPDRRQLARLAERRETWVPSEWVFASKTSEDGKIA
CCCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
EPRIAHNKALAAAELPHVTLHGLRRSFGTLSEWVEVPVGVVAQIQGHKPSAIAEKHYRRR
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHCC
PLDLLRMWHDKVESWMLEQAGVQFQVDPA
CHHHHHHHHHHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure
MVSKQNFTAARVDAFQCEPGKQQTIYWDAKTPGFGLRVTAAGARAFIFESRLFGKTVRLT
CCCCCCCCHHHCEEEECCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCEEEEE
IGDVRAWDLGRARTEAARLKTLIDDGKDPREVRAEQQAEHEARRAEARRQDALFGVVWDE
ECCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHH
YTESRKAFWGERHYRDHLQHADEGGQPKKRGKGVTQPGPLAALRPLKLSELTSERIADWL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
TAESRERPTMSALSFRLLRGFIRWADDTSAYKGIVPIDAYKARDVRDVVPRVKAKDGDSL
HHCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHCCCHHHHHHHCCCCCCCHH
QREQLGVWFREVRKIANPVQSAYLQGLLLTGARREEWAGLRWEDIDFRWRSVVLDDKVEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEECCCCEEEEEEEECCCCC
TGGRTIPLTPYLATLLMNLKRLNETPPDRRQLARLAERRETWVPSEWVFASKTSEDGKIA
CCCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
EPRIAHNKALAAAELPHVTLHGLRRSFGTLSEWVEVPVGVVAQIQGHKPSAIAEKHYRRR
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHCC
PLDLLRMWHDKVESWMLEQAGVQFQVDPA
CHHHHHHHHHHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]