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The map label for this gene is mltB [H]

Identifier: 83720387

GI number: 83720387

Start: 1856064

End: 1857434

Strand: Reverse

Name: mltB [H]

Synonym: BTH_I1647

Alternate gene names: 83720387

Gene position: 1857434-1856064 (Counterclockwise)

Preceding gene: 83718535

Following gene: 83720431

Centisome position: 48.76

GC content: 67.69

Gene sequence:

>1371_bases
ATGTCCGCGATATCGTCTGGTGCGAAGTTCGCCGCTAACTTACCACATCGCGCCCCGCGCCGGGCCGGTGCGACGGGCGT
GTCGGTTATACTGACCCGAAATCATCCGCCTCGTCCGCTTCGCCTCGACATGACCTTCAACCAGCCTGCCGCACCCGTCT
CGCTCGCTTTCCGTCTTCGCTTCCCGCTCGCCGCGCTGTCGCTCGCCGCGACGCTCTTCGCGTCAGCCGCCGTCGCGCAA
ACGCAGCCCGCCGCGACCATCGTCGCGCAAGGGCAGCCGCAGCCGAGCGTGCCGCAAGGGCAAACGTTCGAAGAGGAAAT
CGTTCCGCAGCGCTACGCGAACAATGCGAAGATCGACGCGTTCATCGCCGACATGGTCGCGCGCCACGGCTTCGACGCAA
ACGCGCTGCATGCGCTGTTCGCGCGCGTGAGCTACTCGGCGACCGCCGCGAAGCTCGTGATGCCCGCGCCGTCGCCCGCC
GTCAAGAATTGGCGCGTCTACCAGTCGCGCTTTCTCGACGCGGTGCGCGTGAACGCCGGCGTGAAGTTCTGGCGCGCGAA
CCAGGGCACGCTGCAGCGTGCGTCCACGGAGTTCGGCGTGCCGCCCGAGGTGATCGTCGGCATCATCGGCGTCGAGACGA
TCTACGGCCGCTACATGGGCAACTTCCGCACGCTCGATGCGCTGACGACGCTCGCCTTCGACTACCCGAACACGCCGAAT
CGCGACGCGCGGCAGGCGACGTTCCGCAAGAATCTCGAAGACTTTCTCGTCTGGACGCGCGACAGCCAGCTCGACCCGAC
GAGCGTGCTCGGCTCGTACACGGGCGCGGTCGGCATTCCGCAGTTCCTGCCGAGCAGCATCCGCGATTACGCGGTCGATT
ACGACGGCAATGGCCACATCGATCTGCGCGCGAGCGAGGCCGACGCGATCGGCAGCGTCGCGAACTACCTGAAGCAGCAC
GGCTGGGAAACCAGCCGGCCTGTGGTATGGAACATCGCGCCCGACACGGGCAGCCAGGGCGTCGCGCAAGCGGCGGCCGA
CGGCCGGCCCGAGCCGCACTGGGCGCTTTCACAGTTGCTGCGCGCGGGCCTCGTGCTCGACGAGGCGTCCGTCAACATCG
CCTCGGAGGCGAGCACGCCCGTCACCGTGATCGATCTGCCGACGCCCGGCCGCGCAACCGAATACAAGCTCGGACTGCAG
AACTTCTATGTGCTGACCCGCTACAACCGCAGCTTCTTCTATGCGCTTGCGGTCTACCAACTGGGCGAGCGCGTGAAGGC
GCAAATGGAGGCAAGCGGCGAGCTGATGTCGGCGCCCGCCGACGCCGCCGCTGCGGCACCCGCGCCCGCCGCGCAGCCGC
CGTCCGAATGA

Upstream 100 bases:

>100_bases
CCCCATCTCGTGCAGCAGGCAGTCGGGGTGCGTGTAAAAGGCCGTTGGCATAAGCTCGTTCCGCGACGCGGCCGGTGCGC
GCCGCAGGTCTCCGTCAAGG

Downstream 100 bases:

>100_bases
CGCCGGCACGGCGCGAAGCGCGCAATGGCCCGAATGAGAAAAGCGCCGTAGATGCGGCGCTTTTTTTCGGGTGCCTGCGA
GACGGCGCCGCGCGTTACGC

Product: murein transglycosylase domain-containing protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSAISSGAKFAANLPHRAPRRAGATGVSVILTRNHPPRPLRLDMTFNQPAAPVSLAFRLRFPLAALSLAATLFASAAVAQ
TQPAATIVAQGQPQPSVPQGQTFEEEIVPQRYANNAKIDAFIADMVARHGFDANALHALFARVSYSATAAKLVMPAPSPA
VKNWRVYQSRFLDAVRVNAGVKFWRANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPN
RDARQATFRKNLEDFLVWTRDSQLDPTSVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASEADAIGSVANYLKQH
GWETSRPVVWNIAPDTGSQGVAQAAADGRPEPHWALSQLLRAGLVLDEASVNIASEASTPVTVIDLPTPGRATEYKLGLQ
NFYVLTRYNRSFFYALAVYQLGERVKAQMEASGELMSAPADAAAAAPAPAAQPPSE

Sequences:

>Translated_456_residues
MSAISSGAKFAANLPHRAPRRAGATGVSVILTRNHPPRPLRLDMTFNQPAAPVSLAFRLRFPLAALSLAATLFASAAVAQ
TQPAATIVAQGQPQPSVPQGQTFEEEIVPQRYANNAKIDAFIADMVARHGFDANALHALFARVSYSATAAKLVMPAPSPA
VKNWRVYQSRFLDAVRVNAGVKFWRANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPN
RDARQATFRKNLEDFLVWTRDSQLDPTSVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASEADAIGSVANYLKQH
GWETSRPVVWNIAPDTGSQGVAQAAADGRPEPHWALSQLLRAGLVLDEASVNIASEASTPVTVIDLPTPGRATEYKLGLQ
NFYVLTRYNRSFFYALAVYQLGERVKAQMEASGELMSAPADAAAAAPAPAAQPPSE
>Mature_455_residues
SAISSGAKFAANLPHRAPRRAGATGVSVILTRNHPPRPLRLDMTFNQPAAPVSLAFRLRFPLAALSLAATLFASAAVAQT
QPAATIVAQGQPQPSVPQGQTFEEEIVPQRYANNAKIDAFIADMVARHGFDANALHALFARVSYSATAAKLVMPAPSPAV
KNWRVYQSRFLDAVRVNAGVKFWRANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPNR
DARQATFRKNLEDFLVWTRDSQLDPTSVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASEADAIGSVANYLKQHG
WETSRPVVWNIAPDTGSQGVAQAAADGRPEPHWALSQLLRAGLVLDEASVNIASEASTPVTVIDLPTPGRATEYKLGLQN
FYVLTRYNRSFFYALAVYQLGERVKAQMEASGELMSAPADAAAAAPAPAAQPPSE

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=325, Percent_Identity=40.3076923076923, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 49106; Mature: 48975

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAISSGAKFAANLPHRAPRRAGATGVSVILTRNHPPRPLRLDMTFNQPAAPVSLAFRLR
CCCCCCCCHHHCCCCCCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEH
FPLAALSLAATLFASAAVAQTQPAATIVAQGQPQPSVPQGQTFEEEIVPQRYANNAKIDA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
FIADMVARHGFDANALHALFARVSYSATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAG
HHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
VKFWRANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPN
EEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
RDARQATFRKNLEDFLVWTRDSQLDPTSVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHI
CHHHHHHHHHCHHHHEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEEECCCCCEE
DLRASEADAIGSVANYLKQHGWETSRPVVWNIAPDTGSQGVAQAAADGRPEPHWALSQLL
EEEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHH
RAGLVLDEASVNIASEASTPVTVIDLPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQ
HHCEEEECCCCEEECCCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHH
LGERVKAQMEASGELMSAPADAAAAAPAPAAQPPSE
HHHHHHHHHHHCCCHHCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SAISSGAKFAANLPHRAPRRAGATGVSVILTRNHPPRPLRLDMTFNQPAAPVSLAFRLR
CCCCCCCHHHCCCCCCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEH
FPLAALSLAATLFASAAVAQTQPAATIVAQGQPQPSVPQGQTFEEEIVPQRYANNAKIDA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
FIADMVARHGFDANALHALFARVSYSATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAG
HHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
VKFWRANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPN
EEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
RDARQATFRKNLEDFLVWTRDSQLDPTSVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHI
CHHHHHHHHHCHHHHEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEEECCCCCEE
DLRASEADAIGSVANYLKQHGWETSRPVVWNIAPDTGSQGVAQAAADGRPEPHWALSQLL
EEEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHH
RAGLVLDEASVNIASEASTPVTVIDLPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQ
HHCEEEECCCCEEECCCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHH
LGERVKAQMEASGELMSAPADAAAAAPAPAAQPPSE
HHHHHHHHHHHCCCHHCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]