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The map label for this gene is rimO [H]

Identifier: 83720372

GI number: 83720372

Start: 2541918

End: 2543309

Strand: Direct

Name: rimO [H]

Synonym: BTH_I2259

Alternate gene names: 83720372

Gene position: 2541918-2543309 (Clockwise)

Preceding gene: 83718582

Following gene: 83718975

Centisome position: 66.73

GC content: 65.09

Gene sequence:

>1392_bases
TTGGAAAATTCATTGAAATCAAGCGGTAAGCCGCCCGCCGCGCCCAAGGTGGGCATGGTTTCGCTCGGGTGCCCGAAGGC
GCTCGTCGATTCCGAGCAGATCATCACGCAGTTGCGCGCGGAAGGCTACGAAATCTCCGGCACCTATGACGGCGCGGATC
TCGTCGTCGTCAACACCTGCGGCTTCATCGACGAAGCGGTGCAGGAAAGCCTCGACGCGATCGGCGAAGCGCTCGCCGAA
AACGGCAAGGTGATCGTCACCGGCTGCCTCGGCGCGAAGAAGAGCGCGAGCGGCTCCGGGCTCATCGAGGAAGTCCATCC
GAAGGTGCTCGCCGTCACCGGCCCGCATGCGGTCGGAGAGGTGATGCAGGTCGTGCACAGCCATCTGCCCAAGCCGCACG
ATCCGTTCGTCGATCTCGTGCCGGCCGCGGGCATCAAGCTCACGCCGCGTCACTACGCGTATCTGAAGATCTCCGAAGGC
TGCAATCACCGCTGCTCGTTCTGCATCATTCCGTCGATGCGCGGCGATCTCGTGTCGCGGCCCGTCGCCGAGGTGATGCT
CGAAGCCGAGAACCTGTTCAAGGCCGGCGTGAAGGAACTGCTCGTGATCTCGCAGGACACGAGCGCGTACGGCGTCGACG
TCAAGTACCGCACGGGCTTCTGGAACGGCAGGCCGCTCAAGACGCGAATGACCGAGCTCGTCGGCGCGCTCGGCGAGCTC
GCCGCGCAGTACGGCGCATGGGTGCGGCTGCATTACGTGTATCCGTATCCGCACGTCGACGAGATCATCCCGATGATGGC
GCAAGGCCCGCTCAAGGGCCATGTGCTGCCGTATCTCGACGTGCCGTTCCAGCATGCGCATCCTGACGTGCTCAAGCGAA
TGAAGCGCCCGGCGAACGCGGAGAAGGTGCTCGAGCGCGTGCAGAGGTGGCGCGAGATCTGTCCGGATCTGACGATTCGC
AGCACGTTCATCGCGGGCTTTCCGGGCGAGACCGAAGCGCAATTCGAGACACTCCTCGACTTCATTCGCGAGGCCGAGCT
CGATCGCGTCGGCTGCTTCGCGTATTCTCCGGTCGAGGGCGCGAGCGCGAACGAGCTCGACGGCGCGCTGCCCGACGACG
TGCGCGAGGCGCGCCGCGCGCGCTTCATGGAAGTCGCCGAGGAAGTGTCGGCCGCGCGCATCGAGCGCAAGGTCGGCAAG
ACGCTCAAGGTGCTGATCGACGAGGTCAACGAAGAAGGCGGCATCGGCCGCACCGCGGCCGATGCGCCGGAGATCGACGG
CGTGGTCTATGTGGAGCCGGCGGCGAAGGCGTCCAAGCGCTACAAGGTCGGCGATTTCGTATCCGTGAAGATCACGGGCG
CCGACGGCCACGATCTGTGGGGCGAGGTCTGA

Upstream 100 bases:

>100_bases
GTGCGCCCTCGCAGAAACGTCGCCGCGCGGAGGGTAAAATGCCGGCAAACCCAAGGCGTGCCGCCAGCGGACGCCGTCCC
CTCACGCATGGAAGCAATAG

Downstream 100 bases:

>100_bases
CGATGCCGGCGGCGTTTCCGGACGTGCTCGCGCTCGGCGAGGCGATGGTCGAATTCAACCAGGCGCGGCCGGGCGAGCCG
AACTATCTGCAGGGCGTCGG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MENSLKSSGKPPAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALAE
NGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGEVMQVVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEG
CNHRCSFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPDVLKRMKRPANAEKVLERVQRWREICPDLTIR
STFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEGASANELDGALPDDVREARRARFMEVAEEVSAARIERKVGK
TLKVLIDEVNEEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_463_residues
MENSLKSSGKPPAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALAE
NGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGEVMQVVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEG
CNHRCSFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPDVLKRMKRPANAEKVLERVQRWREICPDLTIR
STFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEGASANELDGALPDDVREARRARFMEVAEEVSAARIERKVGK
TLKVLIDEVNEEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV
>Mature_463_residues
MENSLKSSGKPPAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALAE
NGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGEVMQVVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEG
CNHRCSFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPDVLKRMKRPANAEKVLERVQRWREICPDLTIR
STFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEGASANELDGALPDDVREARRARFMEVAEEVSAARIERKVGK
TLKVLIDEVNEEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=418, Percent_Identity=27.0334928229665, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=24.5901639344262, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI28872784, Length=488, Percent_Identity=24.5901639344262, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=63.6160714285714, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1786882, Length=462, Percent_Identity=25.7575757575758, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=485, Percent_Identity=24.1237113402062, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=26.158940397351, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356207, Length=508, Percent_Identity=26.1811023622047, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922432, Length=468, Percent_Identity=26.4957264957265, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50349; Mature: 50349

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSLKSSGKPPAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
CCCCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECC
GFIDEAVQESLDAIGEALAENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGE
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHCCCEEEEECCHHHHH
VMQVVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSR
HHHHHHHHCCCCCCCHHHHHCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHHC
PVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
CHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPDVLKRMKRPANA
HHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCH
EKVLERVQRWREICPDLTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEG
HHHHHHHHHHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCHHCEEEECCCCC
ASANELDGALPDDVREARRARFMEVAEEVSAARIERKVGKTLKVLIDEVNEEGGIGRTAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCCCEEEECCCHHCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MENSLKSSGKPPAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
CCCCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECC
GFIDEAVQESLDAIGEALAENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGE
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHCCCEEEEECCHHHHH
VMQVVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSR
HHHHHHHHCCCCCCCHHHHHCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHHC
PVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
CHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPDVLKRMKRPANA
HHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCH
EKVLERVQRWREICPDLTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEG
HHHHHHHHHHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCHHCEEEECCCCC
ASANELDGALPDDVREARRARFMEVAEEVSAARIERKVGKTLKVLIDEVNEEGGIGRTAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCCCEEEECCCHHCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA