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The map label for this gene is tycA [H]

Identifier: 83720368

GI number: 83720368

Start: 2205843

End: 2207681

Strand: Direct

Name: tycA [H]

Synonym: BTH_I1953

Alternate gene names: 83720368

Gene position: 2205843-2207681 (Clockwise)

Preceding gene: 83719339

Following gene: 83718863

Centisome position: 57.91

GC content: 72.7

Gene sequence:

>1839_bases
ATGAGCGTTCATGACATGACTCGTGCGAACGATAGCGCACCGGTTCGAGCGGATCGATTCGGCCGGCATTTTGCGACGGC
GGCCGCGGCCGAGATCGCCGCCGCCGATTCGCTGACCGCGCGATTCGCCGACGTGGCCGCGCGCCGAGGCGATGCGCTCG
CGGTGACGTGCGGCGACGAACGCTGGACGTACGGCGCGCTTGCGGCGCGCGCGCGCCGCATCGCCGAAGCGGTTCGCGCG
GCGGGCGAGGCGGGCGGCGAGCCGGTCGCGCTCCTCTATCCGCACGGCGCGCCGATGATCGCCGCGATGTTCGGCGTGCT
CGGCGCCGGCAAGTTCTACGTTCCGTTGATCGCCGATCATCCGCTGCCGCACCTGCAGTCGATCGTGCGCGAGTGCGGCT
GCCGGATCGTGCTCGCCGCGCCCGAGCTCGCCGAGACGGCGGCGCGTCTCGGCGTCGCGGCGCGCGTCATCGACGACGCG
GCCCTGCCGGAAGCGGACGCTCCGCTGGACGCGCGCGACGGCGATGCGGTCTCGTACCTGCTGTTCACGTCCGGCACGAC
CGGCGTGCCGAAGGGCGTGATGCAGTGCGACCGCAACGTGCTGCACCACGCCGCGTGCTATGCGTCGTCGATCGGCCTGA
ACGACGAGGACCGGATGACGCTGTTGCCGTACTACGGCTTCGACGCGTCGGTGATGGACATCTTCGCCACGCTGCTCACG
GGCGCGAGCCTGCATTTATGGGACGTGCGCGAGCGCGGCGTCGACGGCATCGGCGCATGGCTCGCGCGCGAGCGCATGAC
GATCTGGCATTCGACGCCGAGCGTGCTGCGCGCCGCGTTCCCCGCCTTCGCGCGGCCGGCCGCGCTGCGCTGGGCCGTGC
TGGGCGGGGAGGCGGCGACGGGCGGCGACGTCGCGCTCGTCGCGCGGCACGGCGGCCCGCAATGCCGGTTGCTCAACGGC
CTGGGACCGACCGAATGCACGACGGCGCTGCAGTACGTGGCCGATCCCGCCGCCGACACGGGCGTCGCGCGTCTGCCGGT
CGGCCGCCCGGTGCCGGGCGTCGAGGTCGCGCTGCTCGATGCGCAAGGCGAAATCTGCGCGACCGAGGGCGAGCTCGCGA
TCGTCAGCCCCTTCGTCGCGCTCGGATACTGGGGGCGTGCGGACCTGAGCGCCGAGCGCTTCGGCGAGACCGCTCGCCCG
GACGGCGCGCGGCGGTATCGCACGGGCGATCTGCTGCGGATGAACGCGCGCGGCTGCTACGAGCATCTGACGCGGGTCGA
CGATCAGATCAAGATCCGCGGCTTGCGCGTGGAGTTGGGTGAAATCCAGGCGACGATCGGCGCGCACGACGACGTGCTGC
AGGCGGTCGTGCTGCCGCGTCCGGACGACCTCACGCAGCAGCAGACGATCGTCGCCTACGTCGTGCTGCGCGCGGGGGCG
GCCGGCGTCGCGGCGCTGCGCGAGTACGTCGCGGGCCGCCTGCCCGCGTACATGGTGCCGCGCGCGATCGTTCGGCTCGA
CGCGATGCCGCTGCTGCCCAACGGCAAGCTGAATCGTCGCGCGTTGCCGGCGCCGCCGCGAGCCGACGCGGCGGCGGGCG
AGCGCAAGGGGCCGCGCACGCCGCTGCATCGGCTGCTCGCCGCGTGCTGGGCCGACGTGCTGCGACGCGACGCGGTCGGC
ATCGACGAGAACTTCTTCGAGCTCGGCGGCGATTCGCTGCTCGGCGCGCAGTTGCTGTCGCGCGTGAAGCGCGACCTCGA
GCTCGACGCGCGGCTCGGCGACCTGTTCCGTCATCCGACGGTCGAGACGCTGGCCGAACACCTGCTGGCTTCCCGATGA

Upstream 100 bases:

>100_bases
ACGCGCGCCTGTCCGCCGAGCGCGCGGCCGCGGCCGAGGCCGTGGCGTAACGCCGCCGCGCGGTGCCTTCTGTCGTCGAC
AGCGAATAGCGAGACCAACG

Downstream 100 bases:

>100_bases
CCGGCCTCGCGGCCGCGGACATTTCGATGCGATCAACCGATTCAGCGAGACGGACATGACCGACAGCGCGCAGGACAGCA
TCGACGAAGCAACGCGGCCC

Product: peptide synthetase-domain-containing protein

Products: NA

Alternate protein names: Tyrocidine synthase I; ATP-dependent D-phenylalanine adenylase; D-PheA; D-phenylalanine activase; Phenylalanine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MSVHDMTRANDSAPVRADRFGRHFATAAAAEIAAADSLTARFADVAARRGDALAVTCGDERWTYGALAARARRIAEAVRA
AGEAGGEPVALLYPHGAPMIAAMFGVLGAGKFYVPLIADHPLPHLQSIVRECGCRIVLAAPELAETAARLGVAARVIDDA
ALPEADAPLDARDGDAVSYLLFTSGTTGVPKGVMQCDRNVLHHAACYASSIGLNDEDRMTLLPYYGFDASVMDIFATLLT
GASLHLWDVRERGVDGIGAWLARERMTIWHSTPSVLRAAFPAFARPAALRWAVLGGEAATGGDVALVARHGGPQCRLLNG
LGPTECTTALQYVADPAADTGVARLPVGRPVPGVEVALLDAQGEICATEGELAIVSPFVALGYWGRADLSAERFGETARP
DGARRYRTGDLLRMNARGCYEHLTRVDDQIKIRGLRVELGEIQATIGAHDDVLQAVVLPRPDDLTQQQTIVAYVVLRAGA
AGVAALREYVAGRLPAYMVPRAIVRLDAMPLLPNGKLNRRALPAPPRADAAAGERKGPRTPLHRLLAACWADVLRRDAVG
IDENFFELGGDSLLGAQLLSRVKRDLELDARLGDLFRHPTVETLAEHLLASR

Sequences:

>Translated_612_residues
MSVHDMTRANDSAPVRADRFGRHFATAAAAEIAAADSLTARFADVAARRGDALAVTCGDERWTYGALAARARRIAEAVRA
AGEAGGEPVALLYPHGAPMIAAMFGVLGAGKFYVPLIADHPLPHLQSIVRECGCRIVLAAPELAETAARLGVAARVIDDA
ALPEADAPLDARDGDAVSYLLFTSGTTGVPKGVMQCDRNVLHHAACYASSIGLNDEDRMTLLPYYGFDASVMDIFATLLT
GASLHLWDVRERGVDGIGAWLARERMTIWHSTPSVLRAAFPAFARPAALRWAVLGGEAATGGDVALVARHGGPQCRLLNG
LGPTECTTALQYVADPAADTGVARLPVGRPVPGVEVALLDAQGEICATEGELAIVSPFVALGYWGRADLSAERFGETARP
DGARRYRTGDLLRMNARGCYEHLTRVDDQIKIRGLRVELGEIQATIGAHDDVLQAVVLPRPDDLTQQQTIVAYVVLRAGA
AGVAALREYVAGRLPAYMVPRAIVRLDAMPLLPNGKLNRRALPAPPRADAAAGERKGPRTPLHRLLAACWADVLRRDAVG
IDENFFELGGDSLLGAQLLSRVKRDLELDARLGDLFRHPTVETLAEHLLASR
>Mature_611_residues
SVHDMTRANDSAPVRADRFGRHFATAAAAEIAAADSLTARFADVAARRGDALAVTCGDERWTYGALAARARRIAEAVRAA
GEAGGEPVALLYPHGAPMIAAMFGVLGAGKFYVPLIADHPLPHLQSIVRECGCRIVLAAPELAETAARLGVAARVIDDAA
LPEADAPLDARDGDAVSYLLFTSGTTGVPKGVMQCDRNVLHHAACYASSIGLNDEDRMTLLPYYGFDASVMDIFATLLTG
ASLHLWDVRERGVDGIGAWLARERMTIWHSTPSVLRAAFPAFARPAALRWAVLGGEAATGGDVALVARHGGPQCRLLNGL
GPTECTTALQYVADPAADTGVARLPVGRPVPGVEVALLDAQGEICATEGELAIVSPFVALGYWGRADLSAERFGETARPD
GARRYRTGDLLRMNARGCYEHLTRVDDQIKIRGLRVELGEIQATIGAHDDVLQAVVLPRPDDLTQQQTIVAYVVLRAGAA
GVAALREYVAGRLPAYMVPRAIVRLDAMPLLPNGKLNRRALPAPPRADAAAGERKGPRTPLHRLLAACWADVLRRDAVGI
DENFFELGGDSLLGAQLLSRVKRDLELDARLGDLFRHPTVETLAEHLLASR

Specific function: In the first step of peptide synthesis this enzyme activates phenylalanine and racemizes it to the D-isomer [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=440, Percent_Identity=25.9090909090909, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786801, Length=593, Percent_Identity=29.6795952782462, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=25.6461232604374, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=24.1187384044527, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789201, Length=353, Percent_Identity=28.0453257790368, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790505, Length=380, Percent_Identity=26.8421052631579, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=401, Percent_Identity=24.1895261845387, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=436, Percent_Identity=24.0825688073394, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556356, Length=304, Percent_Identity=24.3421052631579, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=650, Percent_Identity=26.4615384615385, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323182, Length=562, Percent_Identity=24.5551601423488, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648676, Length=525, Percent_Identity=24.1904761904762, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581924, Length=430, Percent_Identity=24.8837209302326, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: =5.1.1.11 [H]

Molecular weight: Translated: 65256; Mature: 65125

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHDMTRANDSAPVRADRFGRHFATAAAAEIAAADSLTARFADVAARRGDALAVTCGDE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
RWTYGALAARARRIAEAVRAAGEAGGEPVALLYPHGAPMIAAMFGVLGAGKFYVPLIADH
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCC
PLPHLQSIVRECGCRIVLAAPELAETAARLGVAARVIDDAALPEADAPLDARDGDAVSYL
CCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
LFTSGTTGVPKGVMQCDRNVLHHAACYASSIGLNDEDRMTLLPYYGFDASVMDIFATLLT
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHC
GASLHLWDVRERGVDGIGAWLARERMTIWHSTPSVLRAAFPAFARPAALRWAVLGGEAAT
CCCEEEEEHHHCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCHHEEEEEECCCCCC
GGDVALVARHGGPQCRLLNGLGPTECTTALQYVADPAADTGVARLPVGRPVPGVEVALLD
CCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCEEEEEE
AQGEICATEGELAIVSPFVALGYWGRADLSAERFGETARPDGARRYRTGDLLRMNARGCY
CCCCEEECCCCEEEECHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHH
EHLTRVDDQIKIRGLRVELGEIQATIGAHDDVLQAVVLPRPDDLTQQQTIVAYVVLRAGA
HHHHCCCCCEEEEEEEEEHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
AGVAALREYVAGRLPAYMVPRAIVRLDAMPLLPNGKLNRRALPAPPRADAAAGERKGPRT
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLHRLLAACWADVLRRDAVGIDENFFELGGDSLLGAQLLSRVKRDLELDARLGDLFRHPT
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VETLAEHLLASR
HHHHHHHHHCCC
>Mature Secondary Structure 
SVHDMTRANDSAPVRADRFGRHFATAAAAEIAAADSLTARFADVAARRGDALAVTCGDE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
RWTYGALAARARRIAEAVRAAGEAGGEPVALLYPHGAPMIAAMFGVLGAGKFYVPLIADH
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCC
PLPHLQSIVRECGCRIVLAAPELAETAARLGVAARVIDDAALPEADAPLDARDGDAVSYL
CCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
LFTSGTTGVPKGVMQCDRNVLHHAACYASSIGLNDEDRMTLLPYYGFDASVMDIFATLLT
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHC
GASLHLWDVRERGVDGIGAWLARERMTIWHSTPSVLRAAFPAFARPAALRWAVLGGEAAT
CCCEEEEEHHHCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCHHEEEEEECCCCCC
GGDVALVARHGGPQCRLLNGLGPTECTTALQYVADPAADTGVARLPVGRPVPGVEVALLD
CCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCEEEEEE
AQGEICATEGELAIVSPFVALGYWGRADLSAERFGETARPDGARRYRTGDLLRMNARGCY
CCCCEEECCCCEEEECHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHH
EHLTRVDDQIKIRGLRVELGEIQATIGAHDDVLQAVVLPRPDDLTQQQTIVAYVVLRAGA
HHHHCCCCCEEEEEEEEEHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
AGVAALREYVAGRLPAYMVPRAIVRLDAMPLLPNGKLNRRALPAPPRADAAAGERKGPRT
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLHRLLAACWADVLRRDAVGIDENFFELGGDSLLGAQLLSRVKRDLELDARLGDLFRHPT
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VETLAEHLLASR
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3267240; 9352938; 3032912; 8193142 [H]