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The map label for this gene is kefB [H]

Identifier: 83720313

GI number: 83720313

Start: 543665

End: 545656

Strand: Direct

Name: kefB [H]

Synonym: BTH_I0492

Alternate gene names: 83720313

Gene position: 543665-545656 (Clockwise)

Preceding gene: 83721272

Following gene: 83719836

Centisome position: 14.27

GC content: 69.48

Gene sequence:

>1992_bases
ATGACGCTTCTGCTGCTGCTTGCCTCGGTGGCGGGCGTCGTCGTGTTCCGTTCGCTGAACCTGCCGCCGATGCTCGGCTA
TCTGACGGTCGGCATCGTGGTCGGCCCGCATGCGCTCGGCTTTGCCGCCGACCTGGAGCGCGCCGAGCATCTCGCCGAGT
TCGGCGTCGTGTTCCTGATGTTCTCGATCGGCCTCGAGTTCTCGCTGTCGAAGCTGCGCGCGATGCGCCGGCTCGTGTTC
GGGCTCGGGCTCGCGCAGGTGCTCGGCACGATCGCGATCGCGCTCGTGCTGGGCGCGCTCTTCGAGCGCTGGATGCACAT
CACGTGGGAAGGCAGCGTCGCGCTCGGCGGCGCGCTCGCGATGTCGTCCACCGCGATCGTCTCGAAGATGCTCGCCGAGC
GGCTCGAGATCGAGACCGAGCACGGCCGCAACATCTTCGGCGTGCTGCTGTTCCAGGATCTTGCCGTCGTGCCGCTGCTC
ATCGTGATCGCCGCGTTCGGCGGCGAATCGTCGAGGGATCTCGCGTTGTCGCTCGGGTTCGCGGCCGTGAAGATCGTCGT
CGCGCTCGCGCTGCTCCTGATCGTCGGCCAGCGTTTCATGACGCGCTGGCTGAACGTCGTCGCGCGGCGGCGCTCGCAGG
AGCTGTTCGTGCTGAACCTGCTGCTCGTCACGCTCGGCGCCGCGTTCATCACCGACCGCTTCGGCCTGTCGCTCGCGCTC
GGCGCGTTCATCGCCGGGATGCTGATCTCCGAGACGCCGTACCGGCATCAGGTGGAGGAGGACATCAAGCCGTTTCGCGA
CGTGCTGCTCGGCCTGTTCTTCGTGACGACCGGCATGCTGCTCGATCCGAGCGTGATCTGGCGGCATCCGTTTCTCGTGC
TCGCGTTCTTCGTCGGCCAGGTGCTGCTCAAGAGCGTGATGATCACGGGCCTCGCGCGCGCGTTCGGCGCGACGCCCGGC
GTCGCGATGCGCACGGGGCTCGGGCTCGCGCAGGCGGGCGAATTCGGCTTCGTGCTGCTGAACCTGATTCTCGACCGGCA
TCTCGTCGACGCGACGCTGTTGCAGTCGATCCTCGCGTCGATGCTGCTGTCGATGCTCGCCGCGCCGTTCCTGATCCAGA
ACGCGGACCGGATCGTGATGCGGCTGTCCGCCGCGGAATGGATGCAGCAGTCGCTGCAGATGACGCGGATCGCGACGCAG
AGCATCAAGCAGAAGGGCCACGTGATCGTCTGCGGCTACGGCCGCGCCGGGCAGAACCTCGCGCGGATGCTCGAGCAGGA
GGGGATCGCGTACGTCGCGCTCGACCTCGACCCGGACCGCGTGACGGCCGCCGCCGCGGCGGGCGAATCGGTCGTGTTCG
GCGACGCCGCGCGCCGCGAATCGCTGCTCGCGGCGGGCATCCATCGCGCGGCGGCCGTGGCCGTCACGTACGCGAACACG
CCGTCCGCGCTGCGCGTGCTGCACAACGTGCACGAGCTCGAGCCGACGCTGCCCGCGATCGTGCGCACAGTCGACGACGC
CGATCTCGAGAAGCTGCTCGCCGCGGGCGCGACCGAAGTGATTCCGGAGATCGTCGAAGGCAGCCTGATGCTCGCGTCGC
ACACGCTCGTGCTGATGGGGGTGCCGATGCGGCGCGTCGTGCGGCGCGTCGAGGAAATGCGCGACGAGCGCTACAGCCTG
CTGCGCGGCTACTTCCGCGGCGCCGACGACGCGGACGACGACGACCACGAGCAGGTGCGGCTACAATCGGTGCCCGTCGA
CGCGCGCGCGGACGCGGTCGGCCGCTCGCTCGCCGAGCTGGGGCTTTTCGCCCTTGGCATCGAGGTGACGGCGATTCGCC
GGCACGGGATTCGCGGCGTCGAACCCGACCCCGAGACGAAGCTGCGCGCGAGCGACATCGTCGTGCTGCGCGGGCTGCCC
GAGGCGCTCGCGGAAGCGGAGGAGCGGCTGTCGCGGCATCGCCGCGAAGGCGGATCGACCGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
GCGCCCAATTTTCCGAGTAGTCTCCCGGCCCAACCCGGCGTCCGTCATTGCGGCGCCGTCGACGAACGAGGACGCTTCGC
CCGTGATTTCCCCGCTCGAA

Downstream 100 bases:

>100_bases
CGCCGCGCGGCCGCCGATTTTTTGAGTCGTCGAGCGATCGACGAACGGTGCGGGCATCCGGCGCCGTTTTTTTCGGAGAG
TTTCATGATGTCCACATCGT

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MTLLLLLASVAGVVVFRSLNLPPMLGYLTVGIVVGPHALGFAADLERAEHLAEFGVVFLMFSIGLEFSLSKLRAMRRLVF
GLGLAQVLGTIAIALVLGALFERWMHITWEGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLL
IVIAAFGGESSRDLALSLGFAAVKIVVALALLLIVGQRFMTRWLNVVARRRSQELFVLNLLLVTLGAAFITDRFGLSLAL
GAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWRHPFLVLAFFVGQVLLKSVMITGLARAFGATPG
VAMRTGLGLAQAGEFGFVLLNLILDRHLVDATLLQSILASMLLSMLAAPFLIQNADRIVMRLSAAEWMQQSLQMTRIATQ
SIKQKGHVIVCGYGRAGQNLARMLEQEGIAYVALDLDPDRVTAAAAAGESVVFGDAARRESLLAAGIHRAAAVAVTYANT
PSALRVLHNVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLMGVPMRRVVRRVEEMRDERYSL
LRGYFRGADDADDDDHEQVRLQSVPVDARADAVGRSLAELGLFALGIEVTAIRRHGIRGVEPDPETKLRASDIVVLRGLP
EALAEAEERLSRHRREGGSTAAA

Sequences:

>Translated_663_residues
MTLLLLLASVAGVVVFRSLNLPPMLGYLTVGIVVGPHALGFAADLERAEHLAEFGVVFLMFSIGLEFSLSKLRAMRRLVF
GLGLAQVLGTIAIALVLGALFERWMHITWEGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLL
IVIAAFGGESSRDLALSLGFAAVKIVVALALLLIVGQRFMTRWLNVVARRRSQELFVLNLLLVTLGAAFITDRFGLSLAL
GAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWRHPFLVLAFFVGQVLLKSVMITGLARAFGATPG
VAMRTGLGLAQAGEFGFVLLNLILDRHLVDATLLQSILASMLLSMLAAPFLIQNADRIVMRLSAAEWMQQSLQMTRIATQ
SIKQKGHVIVCGYGRAGQNLARMLEQEGIAYVALDLDPDRVTAAAAAGESVVFGDAARRESLLAAGIHRAAAVAVTYANT
PSALRVLHNVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLMGVPMRRVVRRVEEMRDERYSL
LRGYFRGADDADDDDHEQVRLQSVPVDARADAVGRSLAELGLFALGIEVTAIRRHGIRGVEPDPETKLRASDIVVLRGLP
EALAEAEERLSRHRREGGSTAAA
>Mature_662_residues
TLLLLLASVAGVVVFRSLNLPPMLGYLTVGIVVGPHALGFAADLERAEHLAEFGVVFLMFSIGLEFSLSKLRAMRRLVFG
LGLAQVLGTIAIALVLGALFERWMHITWEGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLI
VIAAFGGESSRDLALSLGFAAVKIVVALALLLIVGQRFMTRWLNVVARRRSQELFVLNLLLVTLGAAFITDRFGLSLALG
AFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWRHPFLVLAFFVGQVLLKSVMITGLARAFGATPGV
AMRTGLGLAQAGEFGFVLLNLILDRHLVDATLLQSILASMLLSMLAAPFLIQNADRIVMRLSAAEWMQQSLQMTRIATQS
IKQKGHVIVCGYGRAGQNLARMLEQEGIAYVALDLDPDRVTAAAAAGESVVFGDAARRESLLAAGIHRAAAVAVTYANTP
SALRVLHNVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLMGVPMRRVVRRVEEMRDERYSLL
RGYFRGADDADDDDHEQVRLQSVPVDARADAVGRSLAELGLFALGIEVTAIRRHGIRGVEPDPETKLRASDIVVLRGLPE
ALAEAEERLSRHRREGGSTAAA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=344, Percent_Identity=28.1976744186047, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789749, Length=543, Percent_Identity=34.0699815837937, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1786232, Length=637, Percent_Identity=30.6122448979592, Blast_Score=245, Evalue=8e-66,
Organism=Escherichia coli, GI1786685, Length=524, Percent_Identity=30.1526717557252, Blast_Score=202, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71707; Mature: 71576

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLLLLASVAGVVVFRSLNLPPMLGYLTVGIVVGPHALGFAADLERAEHLAEFGVVFLM
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FSIGLEFSLSKLRAMRRLVFGLGLAQVLGTIAIALVLGALFERWMHITWEGSVALGGALA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECHHHH
MSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVIAAFGGESSRDLALSLGF
HHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAVKIVVALALLLIVGQRFMTRWLNVVARRRSQELFVLNLLLVTLGAAFITDRFGLSLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWRHPFLVLAFFVGQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHH
VLLKSVMITGLARAFGATPGVAMRTGLGLAQAGEFGFVLLNLILDRHLVDATLLQSILAS
HHHHHHHHHHHHHHHCCCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLSMLAAPFLIQNADRIVMRLSAAEWMQQSLQMTRIATQSIKQKGHVIVCGYGRAGQNL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
ARMLEQEGIAYVALDLDPDRVTAAAAAGESVVFGDAARRESLLAAGIHRAAAVAVTYANT
HHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHEEEEECCC
PSALRVLHNVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLMG
HHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHEEC
VPMRRVVRRVEEMRDERYSLLRGYFRGADDADDDDHEQVRLQSVPVDARADAVGRSLAEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEHHCCCCCHHHHHHHHHHHHH
GLFALGIEVTAIRRHGIRGVEPDPETKLRASDIVVLRGLPEALAEAEERLSRHRREGGST
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
AAA
CCC
>Mature Secondary Structure 
TLLLLLASVAGVVVFRSLNLPPMLGYLTVGIVVGPHALGFAADLERAEHLAEFGVVFLM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FSIGLEFSLSKLRAMRRLVFGLGLAQVLGTIAIALVLGALFERWMHITWEGSVALGGALA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECHHHH
MSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVIAAFGGESSRDLALSLGF
HHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAVKIVVALALLLIVGQRFMTRWLNVVARRRSQELFVLNLLLVTLGAAFITDRFGLSLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWRHPFLVLAFFVGQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHH
VLLKSVMITGLARAFGATPGVAMRTGLGLAQAGEFGFVLLNLILDRHLVDATLLQSILAS
HHHHHHHHHHHHHHHCCCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLSMLAAPFLIQNADRIVMRLSAAEWMQQSLQMTRIATQSIKQKGHVIVCGYGRAGQNL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
ARMLEQEGIAYVALDLDPDRVTAAAAAGESVVFGDAARRESLLAAGIHRAAAVAVTYANT
HHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHEEEEECCC
PSALRVLHNVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLMG
HHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHEEC
VPMRRVVRRVEEMRDERYSLLRGYFRGADDADDDDHEQVRLQSVPVDARADAVGRSLAEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEHHCCCCCHHHHHHHHHHHHH
GLFALGIEVTAIRRHGIRGVEPDPETKLRASDIVVLRGLPEALAEAEERLSRHRREGGST
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
AAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA