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The map label for this gene is purN [H]

Identifier: 83720299

GI number: 83720299

Start: 889411

End: 890073

Strand: Direct

Name: purN [H]

Synonym: BTH_I0772

Alternate gene names: 83720299

Gene position: 889411-890073 (Clockwise)

Preceding gene: 83719346

Following gene: 83719688

Centisome position: 23.35

GC content: 69.68

Gene sequence:

>663_bases
ATGAAAAAAATCGTCATCCTGATTTCCGGCCGCGGCAGCAACATGGAGGCCATCGTACGCGCTTGCGCGCGCGAGGGCTG
GCCTGCCGAGGTTGCCGCCGTGATTTCCAATCGTCCGGGCGCAGCCGGCCTCGAATTCGCGGCCGCGCACGGCATCGCGA
CGGCCGTCGTCGACCACCGCGCGTTCGACGGCCGCGACAGCTTCGATGCGGCGCTCGCCGCCGAGGTCGATCGCTTTGCC
CCCGATCTCGTCGTGCTCGCCGGCTTCATGCGCATTCTCACGCCGGCCTTCGTCGCCAAATATGAGGGCCGGATGCTGAA
CATCCATCCGTCGCTGCTGCCGAGCTTCAAGGGCATCCATACGCACCAGCAGGCGCTCGACGCGGGCGTCGCGCTGCACG
GCGCGTCCGTCCACTTCGTGATTCCGGAGCTCGACAGCGGCGCGATCGTCGCGCAGGCGGCCGTGCCCGTCGTCGCGGGC
GACGGCGCCGATGCGCTCGCTGCGCGCGTGCTCGCCGCCGAGCACGTGCTGTATCCGCGCGCGGTGCGCTGGTTCGTCGA
AGGGAAGCTGCGTCTCGACGCGGGGCGCGCCGTCGTCGCGCCCAACGAGGCGCGCTGGCTGTTCGCGGATGCGATCGACA
CGTCGACGAGCGAGGGCGTATGA

Upstream 100 bases:

>100_bases
AAGCGCGGCCGGATGCCGCAAAGCAAAAGATTATAAACGCTCGGGCGGCCGGGCGGCTCAGGATGCCGGAAACGGCGGGG
GCGAATGGTAAAATCCGCGG

Downstream 100 bases:

>100_bases
AGCTGCACGGTTTCCTGATTGGCCAGACCGAGGCGCTCCTTGCCGACGTCCTGAAATTCGCCGGCCCGGCCGACGTGACG
ACGAGCCGCTTTTTCCGCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKKIVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAAAHGIATAVVDHRAFDGRDSFDAALAAEVDRFA
PDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAG
DGADALAARVLAAEHVLYPRAVRWFVEGKLRLDAGRAVVAPNEARWLFADAIDTSTSEGV

Sequences:

>Translated_220_residues
MKKIVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAAAHGIATAVVDHRAFDGRDSFDAALAAEVDRFA
PDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAG
DGADALAARVLAAEHVLYPRAVRWFVEGKLRLDAGRAVVAPNEARWLFADAIDTSTSEGV
>Mature_220_residues
MKKIVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAAAHGIATAVVDHRAFDGRDSFDAALAAEVDRFA
PDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAG
DGADALAARVLAAEHVLYPRAVRWFVEGKLRLDAGRAVVAPNEARWLFADAIDTSTSEGV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=45.2631578947368, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=45.2631578947368, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=45.2631578947368, Blast_Score=178, Evalue=4e-45,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=54.2105263157895, Blast_Score=214, Evalue=3e-57,
Organism=Escherichia coli, GI1787483, Length=156, Percent_Identity=35.2564102564103, Blast_Score=99, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=41.1111111111111, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=31, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=44.4444444444444, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23117; Mature: 23117

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAAAHGIATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
AFDGRDSFDAALAAEVDRFAPDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIH
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAGDGADALAARVLAAEHVLYPR
HHHHHHHCCEEEECCEEEEEEEECCCCCEEEEECEEEEECCCHHHHHHHHHHHHHHHHHH
AVRWFVEGKLRLDAGRAVVAPNEARWLFADAIDTSTSEGV
HHHHEECCEEEEECCCEEECCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKIVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAAAHGIATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
AFDGRDSFDAALAAEVDRFAPDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIH
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAGDGADALAARVLAAEHVLYPR
HHHHHHHCCEEEECCEEEEEEEECCCCCEEEEECEEEEECCCHHHHHHHHHHHHHHHHHH
AVRWFVEGKLRLDAGRAVVAPNEARWLFADAIDTSTSEGV
HHHHEECCEEEEECCCEEECCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]