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The map label for this gene is rluB [H]

Identifier: 83720156

GI number: 83720156

Start: 2931964

End: 2933472

Strand: Reverse

Name: rluB [H]

Synonym: BTH_I2567

Alternate gene names: 83720156

Gene position: 2933472-2931964 (Counterclockwise)

Preceding gene: 83718805

Following gene: 83720636

Centisome position: 77.01

GC content: 73.23

Gene sequence:

>1509_bases
TTGATCGCGCGCCGCCGCGCGGCCGCGAAATCGAAGAATCCCGAGGCGGCGGAGAGCGCCGACGCACCTCCGGCGGCGGA
AGCCGGGGCGGACGCCGGCGTCGCGAAAGCGCCGGCTCGCGCGCCGCGCAGCAAGGAGGGCGCGGCGAAGGCGCCGCGCA
AGACAGCAGGCAAGCGGGAAGGCGCGGCGCGGCAAGGCGGCCAGGCGAAGCGCGGCGCGCCGGCGGCGGCCGCGCCGTCC
GTCGAGGCTGGCCAGGACGATGTGTTTGCCTATGTGATTTCGCCCGCATTCGATGCCGACAACAACGCGTCGGGCGGCGG
CGTGCGCGCGCCGATGCTGCGCCGGGGCCGCCAGAATCAGCCGAAGCGCGTGCTGTCGCCGGACGACGACGCGCCGAAGT
TGCACAAGGTGCTCGCGGAAGCCGGCATGGGCTCGCGCCGCGAGATGGAGGAGCTCATCATCGCCGGCCGCGTGTCGGTG
AACGGCGAGCCGGCGCATATCGGCCAGCGGATCATGCCGACCGACCAGGTCCGGATCAACGGCAAGCCGGTCAAGCGCAA
GCTGCCGAACAAGCCGCCGCGCGTGCTGCTTTACCACAAGCCGACGGGCGAGATCGTGAGCCACGCGGACCCTGAGGGCC
GCCCGTCGGTGTTTGACCGGCTGCCGCCGATGAAGACCGCGAAATGGCTCGCGGTCGGCCGCCTCGACTTCAATACCGAG
GGCCTGCTGATGCTGACGACGTCCGGCGACCTCGCGAACCGCTTCATGCATCCGCGTTACAGCGTCGAGCGCGAGTATGC
GGTGCGCGTTGTCGGCGAGCTGTCCGAGGCGTCGCGCCAGAAGCTGCTGCACGGCGTCGAGCTCGACGACGGCCCGGCTA
ACTTCCTGCGCATTCGCGACGGAGGCGGCGAAGGCACGAACCACTGGTATCACGTCGCGCTCGCCGAAGGGCGCAACCGC
GAGGTGCGGCGGATGTTCGAGGCGGTCGGCCTGATGGTGAGCCGCCTGATTCGCACGCGTCACGGCCCGATTCCGCTGCC
GCGCGGGTTGAAGCGCGGCCGCTGGGAGGAGCTCGACGAGGCGCAGGTGCGGCGCCTGATGTCGACGGTCGGCCTGAAGG
CGCCGGTCGAGGAGAAGGGCAAGCGCGGCGGCGCGGCCGAGCGCCGCCAGCCCGATCCGATGCAGACGTCGATGGGCTTC
ATCAATCGCGAACCGGTGCTGACGACTCACGGCCAGCTCGACCAGCCGCGGCGCGGCCGGCGCGGCCCGGCGGGCGGCGG
CTTCGGCGCGGGCCTCGGCGGCGGCTATGCCGGCCTGCCGGGCTACGGCGGCGCGCCGCGCCAGGGCGGCCGCGACGTCG
ACGGCAACCGTGCGCCCCACGGAGGCGCGGGCGCGAACAAGCGCGGCGCGGGCAAGGGCGCGCGCAACCCGAACGGCAAT
CGCGCCGAAGGCGGCGCGCGCGGCGGCCAGCGTTCGCCGCAGCAGCGCAATCGTTCGCGCAGCCGCTGA

Upstream 100 bases:

>100_bases
CGTTGCGACGGCGCGCGCCGACGGCGCACCCGAGCAGTCCGCCGCGGACGCGGGCGGCGAAGACCGCCCGCGCCGCGGCC
TGCGGCGCGGGCCGCGCAGC

Downstream 100 bases:

>100_bases
TTGCGCGAGAACGGCATTCGCAACCGCCGCGTGCGTTGCGAATGCCTCGCAAAATCGCCGTTTCGGGCTGATTTTGCGGG
GCCGAGCTTTGCGTTCGGGC

Product: RNA pseudouridylate synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MIARRRAAAKSKNPEAAESADAPPAAEAGADAGVAKAPARAPRSKEGAAKAPRKTAGKREGAARQGGQAKRGAPAAAAPS
VEAGQDDVFAYVISPAFDADNNASGGGVRAPMLRRGRQNQPKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSV
NGEPAHIGQRIMPTDQVRINGKPVKRKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTE
GLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNR
EVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRRLMSTVGLKAPVEEKGKRGGAAERRQPDPMQTSMGF
INREPVLTTHGQLDQPRRGRRGPAGGGFGAGLGGGYAGLPGYGGAPRQGGRDVDGNRAPHGGAGANKRGAGKGARNPNGN
RAEGGARGGQRSPQQRNRSRSR

Sequences:

>Translated_502_residues
MIARRRAAAKSKNPEAAESADAPPAAEAGADAGVAKAPARAPRSKEGAAKAPRKTAGKREGAARQGGQAKRGAPAAAAPS
VEAGQDDVFAYVISPAFDADNNASGGGVRAPMLRRGRQNQPKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSV
NGEPAHIGQRIMPTDQVRINGKPVKRKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTE
GLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNR
EVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRRLMSTVGLKAPVEEKGKRGGAAERRQPDPMQTSMGF
INREPVLTTHGQLDQPRRGRRGPAGGGFGAGLGGGYAGLPGYGGAPRQGGRDVDGNRAPHGGAGANKRGAGKGARNPNGN
RAEGGARGGQRSPQQRNRSRSR
>Mature_502_residues
MIARRRAAAKSKNPEAAESADAPPAAEAGADAGVAKAPARAPRSKEGAAKAPRKTAGKREGAARQGGQAKRGAPAAAAPS
VEAGQDDVFAYVISPAFDADNNASGGGVRAPMLRRGRQNQPKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSV
NGEPAHIGQRIMPTDQVRINGKPVKRKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTE
GLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNR
EVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRRLMSTVGLKAPVEEKGKRGGAAERRQPDPMQTSMGF
INREPVLTTHGQLDQPRRGRRGPAGGGFGAGLGGGYAGLPGYGGAPRQGGRDVDGNRAPHGGAGANKRGAGKGARNPNGN
RAEGGARGGQRSPQQRNRSRSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=269, Percent_Identity=54.6468401486989, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=29.9180327868852, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788510, Length=214, Percent_Identity=30.3738317757009, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081838, Length=195, Percent_Identity=33.8461538461538, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 53679; Mature: 53679

Theoretical pI: Translated: 11.53; Mature: 11.53

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARRRAAAKSKNPEAAESADAPPAAEAGADAGVAKAPARAPRSKEGAAKAPRKTAGKRE
CCCCHHHHHCCCCCCHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
GAARQGGQAKRGAPAAAAPSVEAGQDDVFAYVISPAFDADNNASGGGVRAPMLRRGRQNQ
CCHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCC
PKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRIN
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCEEECCCCHHHHCCCCCCCCEEEEC
GKPVKRKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTE
CCCHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCC
GLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRD
CEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
GGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDE
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
AQVRRLMSTVGLKAPVEEKGKRGGAAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGR
HHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCC
RGPAGGGFGAGLGGGYAGLPGYGGAPRQGGRDVDGNRAPHGGAGANKRGAGKGARNPNGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAEGGARGGQRSPQQRNRSRSR
CCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MIARRRAAAKSKNPEAAESADAPPAAEAGADAGVAKAPARAPRSKEGAAKAPRKTAGKRE
CCCCHHHHHCCCCCCHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
GAARQGGQAKRGAPAAAAPSVEAGQDDVFAYVISPAFDADNNASGGGVRAPMLRRGRQNQ
CCHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCC
PKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRIN
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCEEECCCCHHHHCCCCCCCCEEEEC
GKPVKRKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTE
CCCHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCC
GLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRD
CEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
GGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDE
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
AQVRRLMSTVGLKAPVEEKGKRGGAAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGR
HHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCC
RGPAGGGFGAGLGGGYAGLPGYGGAPRQGGRDVDGNRAPHGGAGANKRGAGKGARNPNGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAEGGARGGQRSPQQRNRSRSR
CCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]