Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 83720108

GI number: 83720108

Start: 953032

End: 953790

Strand: Direct

Name: cobS [H]

Synonym: BTH_I0838

Alternate gene names: 83720108

Gene position: 953032-953790 (Clockwise)

Preceding gene: 83719548

Following gene: 83718473

Centisome position: 25.02

GC content: 72.73

Gene sequence:

>759_bases
ATGAATCCGCTCGCCGAACTGCGCTACTTCGTCGTCGCGCTCGGTTATTTCACGCGCGTGCCGGTGCCGCGCGCGATCAG
CGGCGCGGCAGTCGATCTCGCGCATGCGGCGCGTTACTTTCCGCTCGTCGGCGTGTGCGTCGGCGCGCTTGCCGCGGCCG
TGCATCTTGCCGCGTCGCGCGTGTGGCCGCCGGGCGTCGCGGCGCTCGTGTCGATCGCGGCGACGCTCGTCGCCACGGGC
GCGCTGCACGAGGACGGCCTCGCCGACTGTTGCGATGCGTTCGGCGGCGGCTACACGCGCGAGGACGTGCTGCGCATCAT
GCGCGATTCGCGGATCGGCACGTTCGGCGCGGCGGCGCTCGTCGTCGCGCTCGCGCTGAAATGGCAGGCGCTCGCCGCGA
TGCCGCCCGCGCGCGCCGCGTGGACGATGATCGCCGCGCATGCGGGGAGCCGCACGCTCGCCGCGAGCCTGCTCGTCACG
CTCGACTACGCGCGCGCCGAAGGCAAGGCGCGGCCGGTCGCGCATCGGATGCGCGCGGGCGCGTTCGCGTTCGCGGCCGC
GCTCGGCCTGCCGTGGCTGTTCTGGCCGAACTGGCGCGCGGGCGCGCTCGCGCTCGCGGCGATGCTCGTGTTGCGCGCGG
GCATCGCGCGCTATCTCGGCGCGCGCATCGGCGGCTATACCGGCGATTGCCTCGGCTTCGCGCAGCAGGTGTTCGAGCTT
TCGATCTATCTGGTGATGCTCGGATGGATGTCGTCCTGA

Upstream 100 bases:

>100_bases
CGCGCTCGCGTTGCCGCTCGTGCGCGCGGCGGCGGCGTTCGTCAACGAGATGGCGAGCTTCGAGTCGGCCGGCGTCGCGA
ACCGCGATGCGTGACGCGCG

Downstream 100 bases:

>100_bases
TTCGCCATCCGGCCGTCGCCGTTGACGCGGGCGTCTGCTACGGCAGCAGCGACGTGCCGCTCGCGGCGGGCGCGGCGGCG
GGCGCCGCCGCCATCCTGCG

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNPLAELRYFVVALGYFTRVPVPRAISGAAVDLAHAARYFPLVGVCVGALAAAVHLAASRVWPPGVAALVSIAATLVATG
ALHEDGLADCCDAFGGGYTREDVLRIMRDSRIGTFGAAALVVALALKWQALAAMPPARAAWTMIAAHAGSRTLAASLLVT
LDYARAEGKARPVAHRMRAGAFAFAAALGLPWLFWPNWRAGALALAAMLVLRAGIARYLGARIGGYTGDCLGFAQQVFEL
SIYLVMLGWMSS

Sequences:

>Translated_252_residues
MNPLAELRYFVVALGYFTRVPVPRAISGAAVDLAHAARYFPLVGVCVGALAAAVHLAASRVWPPGVAALVSIAATLVATG
ALHEDGLADCCDAFGGGYTREDVLRIMRDSRIGTFGAAALVVALALKWQALAAMPPARAAWTMIAAHAGSRTLAASLLVT
LDYARAEGKARPVAHRMRAGAFAFAAALGLPWLFWPNWRAGALALAAMLVLRAGIARYLGARIGGYTGDCLGFAQQVFEL
SIYLVMLGWMSS
>Mature_252_residues
MNPLAELRYFVVALGYFTRVPVPRAISGAAVDLAHAARYFPLVGVCVGALAAAVHLAASRVWPPGVAALVSIAATLVATG
ALHEDGLADCCDAFGGGYTREDVLRIMRDSRIGTFGAAALVVALALKWQALAAMPPARAAWTMIAAHAGSRTLAASLLVT
LDYARAEGKARPVAHRMRAGAFAFAAALGLPWLFWPNWRAGALALAAMLVLRAGIARYLGARIGGYTGDCLGFAQQVFEL
SIYLVMLGWMSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=248, Percent_Identity=34.2741935483871, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 26440; Mature: 26440

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLAELRYFVVALGYFTRVPVPRAISGAAVDLAHAARYFPLVGVCVGALAAAVHLAASR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VWPPGVAALVSIAATLVATGALHEDGLADCCDAFGGGYTREDVLRIMRDSRIGTFGAAAL
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHH
VVALALKWQALAAMPPARAAWTMIAAHAGSRTLAASLLVTLDYARAEGKARPVAHRMRAG
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AFAFAAALGLPWLFWPNWRAGALALAAMLVLRAGIARYLGARIGGYTGDCLGFAQQVFEL
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SIYLVMLGWMSS
HHHHHHHHHHCC
>Mature Secondary Structure
MNPLAELRYFVVALGYFTRVPVPRAISGAAVDLAHAARYFPLVGVCVGALAAAVHLAASR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VWPPGVAALVSIAATLVATGALHEDGLADCCDAFGGGYTREDVLRIMRDSRIGTFGAAAL
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHH
VVALALKWQALAAMPPARAAWTMIAAHAGSRTLAASLLVTLDYARAEGKARPVAHRMRAG
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AFAFAAALGLPWLFWPNWRAGALALAAMLVLRAGIARYLGARIGGYTGDCLGFAQQVFEL
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SIYLVMLGWMSS
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA