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The map label for this gene is mrcA [H]

Identifier: 83720103

GI number: 83720103

Start: 2357081

End: 2359603

Strand: Direct

Name: mrcA [H]

Synonym: BTH_I2082

Alternate gene names: 83720103

Gene position: 2357081-2359603 (Clockwise)

Preceding gene: 83719942

Following gene: 83720622

Centisome position: 61.88

GC content: 67.62

Gene sequence:

>2523_bases
ATGCCTATTATCAAACGACCGCCTTCCTCCCGCGCCACGAACGAGCACCATTATTCGCTACGACGTTCGCCCGCCGGCGC
TTATTACACCGATGACGACGATGGCGACGACCACGCGTCGCGCCGCACGAACCGCGACGGCGGCTCGCGCACGTTCGGCT
CGCGCGTCGCGCTGTGGTTCGTCGGGCTTTTCGCGACGCTGCTCGTCGTCGGCGCGCTGATCGTCGGCTATGCGCTCGTC
GTGATGGGGCCGCAGTTGCCGTCGCTCGACGCGCTCACGAACTACCAGCCGAAGGTGCCGTTGCGCGTGTACAGCGCGGA
CCACGTGCTGCTCGGCGAATTCGGCGAGGAGCGCCGCAGCCTCGTGCGCTTCGCGGACATCCCGGACGTGATGAAGAAGG
CGGTGCTCGCGATCGAGGATTACCGTTTCTACGAGCACGGCGGCGTCGACTTCGTCGGCATTCTCCGCGCGGGCGTGGCC
GACCTGCTGCACGGCGGCGCGCGGCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTCGAGCGA
GAAGACGTATACGCGCAAGATCTACGAGATGCTGCTCGCATACAAGATCGAGCGCGCGCTGACGAAGGACCAGATCCTCG
AGCTGTACATGAACCAGATCTATCTGGGCCAGCGTGCGTACGGCTTCGCGGCCGCCGCGCGCGTGTACTTCGGCAAGGAC
CTGAAGGACATCACGCTCGCCGAGGCGGCGATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCGTACAACCCGGTCGTCAA
CCCGAAGCGTGCGAAGGTGCGCCAGGAATATATCCTGAAGCGGATGCTCGAGATCGGCTACATCACGCAGCAGCAGTACG
ACCAGGCGGTGAAGGAGGAAATCCACGTGCGCACGCCGGGCAACCAGTACGCGGTGCACGGCGAATACGTGGCCGAAATG
GTGCGGCAGATGATGTACGCGCAGTATAAGGACGAGACCTACACGCGCGGCCTCACCGTCACGACGACGATCAACGCGGC
CGACCAGGAGGCCGCGTACCAGGCGGTGCGCCGCGGAATCATGGATTACGAGCGTCGCCACGGCTATCGCGGGCCGGAAG
GCTTCGTCGCGCTGCCGCCCGCGGGCGACGGGCGCGACGAGGCGATCGACGACGCGCTCGCCGACCATCCGGACAACGGC
GATCTGCAGTCGGCCGTCGTGCTCGCCGCATCGCCCGCGGCGCTCGACGTGCAGTTCGTCGGCGGCGCGACGGCGACGAT
CAACGCCGCCGGGCTGCGCTTCGCGGCGGGCGCGTTGAGCTCACGCGCGTCGGCCGCGCTGAAGATCAAGCCGGGCTCGA
TCGTGCGCGTGATGAAGGATGCGAAGGGCGCGTGGCAGATCGTGCAGTTGCCGCAAGTCGAAGGCGCGCTCGTGGCGGTC
GCGCCGCAGGACGGCGCAATCCGCTCGCTCGTCGGCGGCTTCGATTTCAACAAGAGCAAGTTCAACCACGTGACGCAGGC
GTGGCGGCAGCCGGGTTCGTCGTTCAAGCCGTTCATCTATTCGGCGTCGCTCGAAAAGGGTCTCGGGCCCGCGACGATCA
TCAACGACGCGCCGCTCTATTTTCCGCCGAGCGTGCCGGGCGGCCAGCCGTGGGAGCCGAAGGACGACGATCAGCCGGAC
GGCCCGATGTCGATGCGCACCGCGTTGCAGCGCTCGAAGAACCTCGTGTCGATCCGGATTCTCGCGTCGATCGGCACGTC
GTATGCGCAGGACTACGTGACGAAGCGCTTCGGCTTCGATCCCGCGAAGACGCCGCCGTATCTGCCGATGGCGCTCGGCG
CGGGCCTCGTCACGCCGCTGCAGCTCGCGGCCGGCTACGCGGTGTTCGCGAACGGCGGCTACAAGGTCGATCCGTATCTG
ATCGCCGAAGTCGACGACGCGCGCGGCCAGGCGCTGCAAAAGGCGCAGCCGGTGGTCGCGGGCGCGAGCGCGACGCGCAC
GATCGACGCGCGCAACGCATACGTGATGAACAGCTTGCTGCACACCGTCGCGACGGCGGGCACGGGCGCCGGCACGAACG
CGCTCGGCCGGGGCGACCTGCAGGGCAAGACGGGTACGACGAACGACGCGAAGGACGGCTGGTTCGCCGGCTACCAGCAA
TCGCTCGTCGCGGTCGCGTGGATGGGCTTCGATCAGCCGAGGAGCCTCGGCAGCCGCGAATTCGGCGCGCAGTTGGCGCT
GCCGATCTGGGTGAACTACATGCGCACCGCGCTTTCGGGCGTGCCGGAGCAGCAGATGGCGATGCCGGACGGGCTGACGA
CGATCGACGGCGAACTGTATTACGCGGATCGCACGCCGGGCGCGGGCTTCATCGCGAACGTCGATTTCAACCCGGCCGCG
AGCCCGACCGTGAGCGCGAACGATGCGCTCGGCTCGGCGGGCGCCGCAGGGCTCACGCCGCCGCCGGTGACGCCGGAGGA
GAAGCGGCAGATCATCGACATGTTCGAAGGCAACAAGCCTTGA

Upstream 100 bases:

>100_bases
GGATCCGTCGACGTGATACGCTCCACGACGCTACGTCCATCGATTCCACGCACGCGCTCAGCGCGTGCTCGAGGCGCGCC
TCGTGCGCCGCCCATCCGAC

Downstream 100 bases:

>100_bases
ACGAATGCCGGTGCGTCGGTCGCATGACACAAGACGGGTGCCGGCGGCACCCGTTTTTTTGCTGGTGCGGGTTCGCGGAT
GGCGGTTGCCGATGTCGCGG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 840; Mature: 839

Protein sequence:

>840_residues
MPIIKRPPSSRATNEHHYSLRRSPAGAYYTDDDDGDDHASRRTNRDGGSRTFGSRVALWFVGLFATLLVVGALIVGYALV
VMGPQLPSLDALTNYQPKVPLRVYSADHVLLGEFGEERRSLVRFADIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVA
DLLHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRAYGFAAAARVYFGKD
LKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEIGYITQQQYDQAVKEEIHVRTPGNQYAVHGEYVAEM
VRQMMYAQYKDETYTRGLTVTTTINAADQEAAYQAVRRGIMDYERRHGYRGPEGFVALPPAGDGRDEAIDDALADHPDNG
DLQSAVVLAASPAALDVQFVGGATATINAAGLRFAAGALSSRASAALKIKPGSIVRVMKDAKGAWQIVQLPQVEGALVAV
APQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLEKGLGPATIINDAPLYFPPSVPGGQPWEPKDDDQPD
GPMSMRTALQRSKNLVSIRILASIGTSYAQDYVTKRFGFDPAKTPPYLPMALGAGLVTPLQLAAGYAVFANGGYKVDPYL
IAEVDDARGQALQKAQPVVAGASATRTIDARNAYVMNSLLHTVATAGTGAGTNALGRGDLQGKTGTTNDAKDGWFAGYQQ
SLVAVAWMGFDQPRSLGSREFGAQLALPIWVNYMRTALSGVPEQQMAMPDGLTTIDGELYYADRTPGAGFIANVDFNPAA
SPTVSANDALGSAGAAGLTPPPVTPEEKRQIIDMFEGNKP

Sequences:

>Translated_840_residues
MPIIKRPPSSRATNEHHYSLRRSPAGAYYTDDDDGDDHASRRTNRDGGSRTFGSRVALWFVGLFATLLVVGALIVGYALV
VMGPQLPSLDALTNYQPKVPLRVYSADHVLLGEFGEERRSLVRFADIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVA
DLLHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRAYGFAAAARVYFGKD
LKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEIGYITQQQYDQAVKEEIHVRTPGNQYAVHGEYVAEM
VRQMMYAQYKDETYTRGLTVTTTINAADQEAAYQAVRRGIMDYERRHGYRGPEGFVALPPAGDGRDEAIDDALADHPDNG
DLQSAVVLAASPAALDVQFVGGATATINAAGLRFAAGALSSRASAALKIKPGSIVRVMKDAKGAWQIVQLPQVEGALVAV
APQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLEKGLGPATIINDAPLYFPPSVPGGQPWEPKDDDQPD
GPMSMRTALQRSKNLVSIRILASIGTSYAQDYVTKRFGFDPAKTPPYLPMALGAGLVTPLQLAAGYAVFANGGYKVDPYL
IAEVDDARGQALQKAQPVVAGASATRTIDARNAYVMNSLLHTVATAGTGAGTNALGRGDLQGKTGTTNDAKDGWFAGYQQ
SLVAVAWMGFDQPRSLGSREFGAQLALPIWVNYMRTALSGVPEQQMAMPDGLTTIDGELYYADRTPGAGFIANVDFNPAA
SPTVSANDALGSAGAAGLTPPPVTPEEKRQIIDMFEGNKP
>Mature_839_residues
PIIKRPPSSRATNEHHYSLRRSPAGAYYTDDDDGDDHASRRTNRDGGSRTFGSRVALWFVGLFATLLVVGALIVGYALVV
MGPQLPSLDALTNYQPKVPLRVYSADHVLLGEFGEERRSLVRFADIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVAD
LLHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRAYGFAAAARVYFGKDL
KDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEIGYITQQQYDQAVKEEIHVRTPGNQYAVHGEYVAEMV
RQMMYAQYKDETYTRGLTVTTTINAADQEAAYQAVRRGIMDYERRHGYRGPEGFVALPPAGDGRDEAIDDALADHPDNGD
LQSAVVLAASPAALDVQFVGGATATINAAGLRFAAGALSSRASAALKIKPGSIVRVMKDAKGAWQIVQLPQVEGALVAVA
PQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLEKGLGPATIINDAPLYFPPSVPGGQPWEPKDDDQPDG
PMSMRTALQRSKNLVSIRILASIGTSYAQDYVTKRFGFDPAKTPPYLPMALGAGLVTPLQLAAGYAVFANGGYKVDPYLI
AEVDDARGQALQKAQPVVAGASATRTIDARNAYVMNSLLHTVATAGTGAGTNALGRGDLQGKTGTTNDAKDGWFAGYQQS
LVAVAWMGFDQPRSLGSREFGAQLALPIWVNYMRTALSGVPEQQMAMPDGLTTIDGELYYADRTPGAGFIANVDFNPAAS
PTVSANDALGSAGAAGLTPPPVTPEEKRQIIDMFEGNKP

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=814, Percent_Identity=39.6805896805897, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=36.9477911646586, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788867, Length=265, Percent_Identity=31.6981132075472, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789601, Length=172, Percent_Identity=34.3023255813954, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 90792; Mature: 90661

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIKRPPSSRATNEHHYSLRRSPAGAYYTDDDDGDDHASRRTNRDGGSRTFGSRVALWF
CCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHH
VGLFATLLVVGALIVGYALVVMGPQLPSLDALTNYQPKVPLRVYSADHVLLGEFGEERRS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEECCCEEEECHHHHHHHH
LVRFADIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVADLLHGGARQGASTITMQVAR
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRAYGFAAAARVYFGKD
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
LKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEIGYITQQQYDQAVKEE
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHVRTPGNQYAVHGEYVAEMVRQMMYAQYKDETYTRGLTVTTTINAADQEAAYQAVRRGI
EEEECCCCCEEECHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCHHHHHHHHHHHHH
MDYERRHGYRGPEGFVALPPAGDGRDEAIDDALADHPDNGDLQSAVVLAASPAALDVQFV
HHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEE
GGATATINAAGLRFAAGALSSRASAALKIKPGSIVRVMKDAKGAWQIVQLPQVEGALVAV
CCCCEEEECCCHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEEEECCCCCCEEEEE
APQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLEKGLGPATIINDAPLY
ECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCEEEECCCCCE
FPPSVPGGQPWEPKDDDQPDGPMSMRTALQRSKNLVSIRILASIGTSYAQDYVTKRFGFD
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHCHHHHHHHHHHHCCCC
PAKTPPYLPMALGAGLVTPLQLAAGYAVFANGGYKVDPYLIAEVDDARGQALQKAQPVVA
CCCCCCCCHHHHCCCHHHHHHHHCCEEEEECCCEEECCEEEEECCCHHHHHHHHCCCCEE
GASATRTIDARNAYVMNSLLHTVATAGTGAGTNALGRGDLQGKTGTTNDAKDGWFAGYQQ
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SLVAVAWMGFDQPRSLGSREFGAQLALPIWVNYMRTALSGVPEQQMAMPDGLTTIDGELY
HHHHHHHCCCCCHHHCCCHHHCCEEEHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCEEE
YADRTPGAGFIANVDFNPAASPTVSANDALGSAGAAGLTPPPVTPEEKRQIIDMFEGNKP
EECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PIIKRPPSSRATNEHHYSLRRSPAGAYYTDDDDGDDHASRRTNRDGGSRTFGSRVALWF
CCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHH
VGLFATLLVVGALIVGYALVVMGPQLPSLDALTNYQPKVPLRVYSADHVLLGEFGEERRS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEECCCEEEECHHHHHHHH
LVRFADIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVADLLHGGARQGASTITMQVAR
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRAYGFAAAARVYFGKD
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
LKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEIGYITQQQYDQAVKEE
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHVRTPGNQYAVHGEYVAEMVRQMMYAQYKDETYTRGLTVTTTINAADQEAAYQAVRRGI
EEEECCCCCEEECHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCHHHHHHHHHHHHH
MDYERRHGYRGPEGFVALPPAGDGRDEAIDDALADHPDNGDLQSAVVLAASPAALDVQFV
HHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEE
GGATATINAAGLRFAAGALSSRASAALKIKPGSIVRVMKDAKGAWQIVQLPQVEGALVAV
CCCCEEEECCCHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEEEECCCCCCEEEEE
APQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLEKGLGPATIINDAPLY
ECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCEEEECCCCCE
FPPSVPGGQPWEPKDDDQPDGPMSMRTALQRSKNLVSIRILASIGTSYAQDYVTKRFGFD
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHCHHHHHHHHHHHCCCC
PAKTPPYLPMALGAGLVTPLQLAAGYAVFANGGYKVDPYLIAEVDDARGQALQKAQPVVA
CCCCCCCCHHHHCCCHHHHHHHHCCEEEEECCCEEECCEEEEECCCHHHHHHHHCCCCEE
GASATRTIDARNAYVMNSLLHTVATAGTGAGTNALGRGDLQGKTGTTNDAKDGWFAGYQQ
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SLVAVAWMGFDQPRSLGSREFGAQLALPIWVNYMRTALSGVPEQQMAMPDGLTTIDGELY
HHHHHHHCCCCCHHHCCCHHHCCEEEHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCEEE
YADRTPGAGFIANVDFNPAASPTVSANDALGSAGAAGLTPPPVTPEEKRQIIDMFEGNKP
EECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA