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The map label for this gene is nuoF2 [H]

Identifier: 83719980

GI number: 83719980

Start: 1830011

End: 1831579

Strand: Reverse

Name: nuoF2 [H]

Synonym: BTH_I1623

Alternate gene names: 83719980

Gene position: 1831579-1830011 (Counterclockwise)

Preceding gene: 83721159

Following gene: 83718922

Centisome position: 48.08

GC content: 71.89

Gene sequence:

>1569_bases
ATGAGCACGCGCATCCATGTGCCGCGCGATTCCGCTGCGCTCGCGGTCGGCGCGGACGCGCTCGCGCGCGCGATCGAGGC
GGAGGCGGCAAGGCGCGGCGTCGCGATCGAGCTCGTGCGCAACGGCTCGCGCGGGCTTCTGTGGCTCGAGCCGCTCGTCG
AAGTGGGCACCGCGGCGGGCCGCGTCGGCTACGCGAATCTCTCGGCGGGCGACGTCCCCGCGCTCTTCGACGCCGGCTGG
CTCGAAGGCGGCGAACATCCGGCGCGCGTCGGCATCGTCGACGAAATCCCTTATCTGAAGCGCCAGCAGCGGCTCACTTT
CGCGCGCATCGGCATCACCGATCCCCTGTCGGTCGACGACTACGTCGCGCACGGCGGCCTCGAAGGCCTGAAGAACGCGC
TCGCGCTCGACGGCGCCGCCGCGTGCGAGACGCTCGTCGAATCGGGCCTGCGCGGACGCGGCGGCGCCGCGTTCCCGGCG
GGCATCAAGTGGCGGACCGTGCGCGACGCCGCGGCCGCGCAGAAATACATCGTCTGCAACGCGGACGAAGGCGATTCGGG
CACGTTCTCCGATCGCCTCGTGATGGAAAGCGATCCGTACTGCCTGATCGAAGGGATGATCATCGCCGGCATCGCGACGG
GCGCGACGATCGGCCATATCTACGTGCGCAGCGAATATCCGCACGCGATCGCGGCGCTCGGCGAGGCGATCGCCCGCTCG
CGCGCGGCCGGCTGGCTCGGCGCGAGCGTGCTCGGCACCGCGCACGCGTTCGAGCTGCACGTCGCGAAAGGCGCCGGCGC
GTACGTCTGCGGCGAGGAGACGGCGCTCCTCGAATCGCTCGAAGGCAAGCGCGGCGTCGTGCGCGCGAAGCCGCCGGTGC
CCGCGCTGTCGGGGCTATTCGGTCAGCCGACCGTCATCAACAACGTGATCACGCTCGCGACCGTGCCGATCATCTTCGCG
CGCGGCGCGGCGTTCTATCGCGATTACGGGACGGGCCGCTCGCGCGGCACGCTGCCGTTCCAGCTCGCGGGCAACGTGAA
GCGCGGCGGGCTCGTCGAGCTCGCGTTCGGCGTCACGCTGCGCGAGCTGCTGTTCGACTTCGGCGGCGGCACCGCGAGCG
GGCGTCCCGCTCGCGCCGCGCAGGTCGGCGGCCCGCTCGGCACCTACCTGCCCGACAGCCAGTGGGACATCCCGCTCGAC
TACGAGGCGTACGCGGCCGTCGGCGCCGTCGTCGGGCACGGCGGCATCGTGCTGCACGACGACACGTCGAACCTCGCCGC
GCTCGCCGAATACGCGATGCACTTCTGCGCGCTCGAATCGTGCGGCAAGTGCACGCCGTGCCGGATCGGCTCGACGCGCG
GCGTCGAGACGATCGCGAAGATCCGCGCGGGCGACACGTCGGAAAAGCAGGTGCGCCTGCTGCGCGAGCTCTGCGACACG
ATGACCGCGGGCTCGCTTTGCGCGATGGGCGGCATGACGCCGTACCCGGTGCTGTCCGCGCTCGATCACTTCCCCGAAGA
TTTCGGCCTCGCGCCGCCCGCGAGCGGCGCGGCGCCCGCCACGGCCTGA

Upstream 100 bases:

>100_bases
AGTCGCCATCGCTCACGATCAACGACGAGCCGCACGCGAAAATGTCGCCCGAGCGCTTCGACGCGCTCTTCGACGCGGCT
GTCCGCACCAAGGAGCCCGC

Downstream 100 bases:

>100_bases
CAGCAGGAGTTCCGATGACTTCTTCCGCATTCGATTCGTCCCGGCAAGGCTGCGGCTCCGGCCAGTGCGCGTGCAAGAGC
GCCGCGCACGCGCGTCGCGC

Product: formate dehydrogenase subunit beta

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSTRIHVPRDSAALAVGADALARAIEAEAARRGVAIELVRNGSRGLLWLEPLVEVGTAAGRVGYANLSAGDVPALFDAGW
LEGGEHPARVGIVDEIPYLKRQQRLTFARIGITDPLSVDDYVAHGGLEGLKNALALDGAAACETLVESGLRGRGGAAFPA
GIKWRTVRDAAAAQKYIVCNADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARS
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPVPALSGLFGQPTVINNVITLATVPIIFA
RGAAFYRDYGTGRSRGTLPFQLAGNVKRGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPDSQWDIPLD
YEAYAAVGAVVGHGGIVLHDDTSNLAALAEYAMHFCALESCGKCTPCRIGSTRGVETIAKIRAGDTSEKQVRLLRELCDT
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAPPASGAAPATA

Sequences:

>Translated_522_residues
MSTRIHVPRDSAALAVGADALARAIEAEAARRGVAIELVRNGSRGLLWLEPLVEVGTAAGRVGYANLSAGDVPALFDAGW
LEGGEHPARVGIVDEIPYLKRQQRLTFARIGITDPLSVDDYVAHGGLEGLKNALALDGAAACETLVESGLRGRGGAAFPA
GIKWRTVRDAAAAQKYIVCNADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARS
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPVPALSGLFGQPTVINNVITLATVPIIFA
RGAAFYRDYGTGRSRGTLPFQLAGNVKRGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPDSQWDIPLD
YEAYAAVGAVVGHGGIVLHDDTSNLAALAEYAMHFCALESCGKCTPCRIGSTRGVETIAKIRAGDTSEKQVRLLRELCDT
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAPPASGAAPATA
>Mature_521_residues
STRIHVPRDSAALAVGADALARAIEAEAARRGVAIELVRNGSRGLLWLEPLVEVGTAAGRVGYANLSAGDVPALFDAGWL
EGGEHPARVGIVDEIPYLKRQQRLTFARIGITDPLSVDDYVAHGGLEGLKNALALDGAAACETLVESGLRGRGGAAFPAG
IKWRTVRDAAAAQKYIVCNADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARSR
AAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPVPALSGLFGQPTVINNVITLATVPIIFAR
GAAFYRDYGTGRSRGTLPFQLAGNVKRGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPDSQWDIPLDY
EAYAAVGAVVGHGGIVLHDDTSNLAALAEYAMHFCALESCGKCTPCRIGSTRGVETIAKIRAGDTSEKQVRLLRELCDTM
TAGSLCAMGGMTPYPVLSALDHFPEDFGLAPPASGAAPATA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=363, Percent_Identity=37.1900826446281, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI260656005, Length=363, Percent_Identity=37.1900826446281, Blast_Score=242, Evalue=5e-64,
Organism=Escherichia coli, GI1788620, Length=418, Percent_Identity=38.9952153110048, Blast_Score=295, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17531727, Length=420, Percent_Identity=35.7142857142857, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19920794, Length=363, Percent_Identity=38.0165289256198, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45550452, Length=363, Percent_Identity=38.2920110192837, Blast_Score=233, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24653819, Length=352, Percent_Identity=30.9659090909091, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=28.9156626506024, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54219; Mature: 54088

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRIHVPRDSAALAVGADALARAIEAEAARRGVAIELVRNGSRGLLWLEPLVEVGTAAG
CCCEEECCCCCCEEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEHHHHHHHCCCCC
RVGYANLSAGDVPALFDAGWLEGGEHPARVGIVDEIPYLKRQQRLTFARIGITDPLSVDD
CCCCCCCCCCCCCHHHHCCCCCCCCCCCEECCHHCCCHHHHHHCCEEEEECCCCCCCCHH
YVAHGGLEGLKNALALDGAAACETLVESGLRGRGGAAFPAGIKWRTVRDAAAAQKYIVCN
HHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHCCEEEEEE
ADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARS
CCCCCCCCCCCEEEECCCCEEEEECCCEEHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPVPALSGLF
HHHHHHHHHHHHCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCHHHCCC
GQPTVINNVITLATVPIIFARGAAFYRDYGTGRSRGTLPFQLAGNVKRGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHH
RELLFDFGGGTASGRPARAAQVGGPLGTYLPDSQWDIPLDYEAYAAVGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
DTSNLAALAEYAMHFCALESCGKCTPCRIGSTRGVETIAKIRAGDTSEKQVRLLRELCDT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAPPASGAAPATA
HCCCCHHHHCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STRIHVPRDSAALAVGADALARAIEAEAARRGVAIELVRNGSRGLLWLEPLVEVGTAAG
CCEEECCCCCCEEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEHHHHHHHCCCCC
RVGYANLSAGDVPALFDAGWLEGGEHPARVGIVDEIPYLKRQQRLTFARIGITDPLSVDD
CCCCCCCCCCCCCHHHHCCCCCCCCCCCEECCHHCCCHHHHHHCCEEEEECCCCCCCCHH
YVAHGGLEGLKNALALDGAAACETLVESGLRGRGGAAFPAGIKWRTVRDAAAAQKYIVCN
HHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHCCEEEEEE
ADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARS
CCCCCCCCCCCEEEECCCCEEEEECCCEEHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPVPALSGLF
HHHHHHHHHHHHCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCHHHCCC
GQPTVINNVITLATVPIIFARGAAFYRDYGTGRSRGTLPFQLAGNVKRGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHH
RELLFDFGGGTASGRPARAAQVGGPLGTYLPDSQWDIPLDYEAYAAVGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
DTSNLAALAEYAMHFCALESCGKCTPCRIGSTRGVETIAKIRAGDTSEKQVRLLRELCDT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAPPASGAAPATA
HCCCCHHHHCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]