Click here to switch to the map view.

The map label for this gene is pcaF [H]

Identifier: 83719929

GI number: 83719929

Start: 3336571

End: 3337773

Strand: Direct

Name: pcaF [H]

Synonym: BTH_I2901

Alternate gene names: 83719929

Gene position: 3336571-3337773 (Clockwise)

Preceding gene: 83718561

Following gene: 83718849

Centisome position: 87.59

GC content: 71.65

Gene sequence:

>1203_bases
ATGACCAAAGCATTCCTGTGTGACGCAATCCGAACGCCCATCGGCCGCTACGGCGGCGCGCTGGCCCCGGTGCGGGCCGA
CGACCTCGGCGCGGTGCCGCTCAAGGCGCTCGCCGAGCGCAACCGCGACGTCGACTGGGCCGCCGTCGACGACGTGATCT
ACGGCTGCGCGAACCAGGCCGGCGAAGACAACCGCAACGTCGCGCGCATGTCGCTGCTGCTCGCCGGCTTGCCCCACGCC
GTGCCCGGCGCGACGATCAACCGCCTCTGCGGCTCCGGGATGGACGCGATCGGCATCGCCGCGCGCGCGATCAAGGCCGG
CGAGGCGAACCTGATGATCGCGGGCGGCGTCGAGAGCATGAGCCGTGCGCCGTTCGTCACGGGCAAGGCGACGAGCGCGT
TCTCGCGGCAGGCCGAGATCTTCGATACGACGATCGGCTGGCGCTTCGTCAATCCGCTGATGAAACAGCAGTATGGCGTC
GATTCGATGCCGGAGACGGCCGAGCACGTCGCGACCGATTACCGCGTGAGCCGCGCCGATCAGGACGCGTTCGCGCTGCG
CAGCCAGCAGAAGGCGGCGCGCGCGCAGGCCGACGGCACCCTCGCGCAGGAAATCGTGCCGGTGACGATCGCGCAGAAGA
AGGGCGAGCCGCTCGTCGTGGCGCGCGACGAGCATCCGCGCGAGACGACGCTCGATGCGCTCGCCAAACTCAAGGGCGTC
GTGCGGCCGGACGGCACGGTGACGGCAGGCAACGCGTCGGGCGTGAACGACGGCGCGTGCGCGCTGCTCGTCGCGAGCGA
GGCGGCCGCGCGGCGTCACGGCCTCGTGCCGCGCGCGCGCGTGCTGGGGATCGCGACCGCGGGCGTCGAGCCGCGGGTGA
TGGGGATCGGCCCGGCGCCGGCGACGCAGAAGCTGCTCGCGCGGCTTGGCATGACGCTCGACCAGTTCGACGTGATCGAG
CTGAACGAGGCGTTCGCATCGCAGGGCCTGGCGGTGCTGCGGCTGCTGGGCGTCGCCGACGACGATGCGCGCGTGAATCC
GAACGGCGGCGCGATCGCGCTCGGCCATCCGCTCGGCGCCTCGGGCGCGCGGCTCGTGACGAGCGCGACGTATCAGTTGC
ATCGCACCGGCGGCCGCTTCGCGCTCTGCACGATGTGCATCGGCGTCGGGCAGGGCATCGCGATCGCGATCGAGCGCGTA
TAA

Upstream 100 bases:

>100_bases
CAAACGGCATAACCCGTTCGGCCGAGCTGCCGATCGCGCGATCGTTCACTACCATGAATGAACCCGCCCGCTCGCGGGTT
CACCCTTGCCGGAACTCGAC

Downstream 100 bases:

>100_bases
GACGAGCCGGCAGGCGAAGGACGCATCCCGTTCATTTCGAAGGCATGACAGGAGACAGCCGATGTCCTACCAAGCGATTC
GCATCGACATCGATCCGGCA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTKAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALAERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAGLPHA
VPGATINRLCGSGMDAIGIAARAIKAGEANLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQQYGV
DSMPETAEHVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIVPVTIAQKKGEPLVVARDEHPRETTLDALAKLKGV
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQFDVIE
LNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTSATYQLHRTGGRFALCTMCIGVGQGIAIAIERV

Sequences:

>Translated_400_residues
MTKAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALAERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAGLPHA
VPGATINRLCGSGMDAIGIAARAIKAGEANLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQQYGV
DSMPETAEHVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIVPVTIAQKKGEPLVVARDEHPRETTLDALAKLKGV
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQFDVIE
LNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTSATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
>Mature_399_residues
TKAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALAERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAGLPHAV
PGATINRLCGSGMDAIGIAARAIKAGEANLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQQYGVD
SMPETAEHVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIVPVTIAQKKGEPLVVARDEHPRETTLDALAKLKGVV
RPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQFDVIEL
NEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTSATYQLHRTGGRFALCTMCIGVGQGIAIAIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=41.7721518987342, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.2871536523929, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=33.7408312958435, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI4501853, Length=339, Percent_Identity=41.2979351032448, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.3817330210773, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI194353979, Length=337, Percent_Identity=29.9703264094956, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.3291770573566, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=45.6359102244389, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.409429280397, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.7160493827161, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=31.7073170731707, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=44.2211055276382, Blast_Score=303, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI25147385, Length=403, Percent_Identity=34.7394540942928, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=34.7290640394089, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=32.093023255814, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=29.2079207920792, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=35.6265356265356, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322031, Length=339, Percent_Identity=35.3982300884956, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=42.643391521197, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=38.6699507389163, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=35.0617283950617, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.1320754716981, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24585051, Length=320, Percent_Identity=25.9375, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921506, Length=320, Percent_Identity=25.3125, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41685; Mature: 41554

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALAERNRDVDWAAVDDVIYGCANQA
CCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGIAARAIKAGEANLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQQYGVDSMPETAEHVATDYRVSRAD
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
QDAFALRSQQKAARAQADGTLAQEIVPVTIAQKKGEPLVVARDEHPRETTLDALAKLKGV
CHHHHHHHHHHHHHHHCCCHHHHHHEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEECCCCC
ATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARLVTSATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TKAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALAERNRDVDWAAVDDVIYGCANQA
CHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGIAARAIKAGEANLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQQYGVDSMPETAEHVATDYRVSRAD
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
QDAFALRSQQKAARAQADGTLAQEIVPVTIAQKKGEPLVVARDEHPRETTLDALAKLKGV
CHHHHHHHHHHHHHHHCCCHHHHHHEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEECCCCC
ATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARLVTSATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]