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The map label for this gene is cyaE [H]

Identifier: 83719927

GI number: 83719927

Start: 3392434

End: 3393873

Strand: Direct

Name: cyaE [H]

Synonym: BTH_I2950

Alternate gene names: 83719927

Gene position: 3392434-3393873 (Clockwise)

Preceding gene: 83719024

Following gene: 83721600

Centisome position: 89.06

GC content: 67.64

Gene sequence:

>1440_bases
ATGAAGCCCGCACGCATTCTCGCCATGGCCGGCGCGCTCGCGCTCTTCGCGTCGCAAGCGACACACGCGCAATGGCTCGA
TCTCTACGGAACCCGAAAGAATGTGTCGGATTCTCCCGCCGCGCCGATGCTGAAGAACGCGACATGCCAGCCCGAGCTCG
CGGACCGTCCGATCGAGCTCGAAGACGCGATCCTGCAGGCGATCTGCGCGAACCCGCAGGCACGGCGCGCGTGGGCCGAT
GCGCGCGCGCAGGCCGCACAGCTCGGCGTGAAGGAGGCAGCGTATCTGCCGACGCTCAATGCAACAGCGGGCATTCAGCG
CAACTGGCAAACGACGACGTATGAGCAGAACCTCGGCCCGATCACGCTCACCGCCGACCAGAAGCAGATCCAGACGAGCC
GCTACGGCGCGTTGAACCTGAGCTGGGTGCTGTTCGATTTCGGCAAGCGCAGCGCGGCGCTCAGGCAGGCGCGCGAGCTC
CTCGCCGCCGCCAACGCGACGCAGGACGACACGCTCCAGACCATCCTGTTCAATGCCGCCCAGGCGTATTACAACCTGCG
CGACACGCAGGCGGCGCTCGACGCGGCGCGCACGACCGAACGCGTCGCGCAGGATAGCCTCACTGCGGCGAAGGCGAAGC
ACGACGCCGGCGCGGGCACGCTGAGCGATCAGTTGCAGGCGCAAACGAGCTATCGCCGCGCGGTGCTCGATCGTGTGAGC
GCCGAAGGCGACGTGCAGAACGCGACGGGCGTCCTCGCCGTCGCGATGGGGCTCGACGCGAACACGCCGCTGCGGATCGC
GACCGCCGAGCCGCCGGTCGATCGCAACGCGTTCGCCGAAGGCATCGCGCAATTGATCGACGAGGCGAAGCGGCAGCATC
CGAAGCTCGTCGCCGCGCGCGCGAAACTCGACGCGGCGCGCGCGAACGTCGATGCGGTGCGTGCTCAGGGCCGCCCGACC
ATCTCGTTGACCGGCAGCCTCAGCCGCAACAGCCCGTCCTATCAGCAACAGCCGGGCATCCAGACCACCGCGAGCCACGG
CAGCATGATCGGCGTGCAGGTGACGATTCCGCTGTTCGAGGGCTTCGCGTCGGGCTACCGCGTCGCGGCCGCGCAAGCGC
AGGCGGATGCGCAGGAGGCCGATGTGCAGAACACCGAGTTGAACGTATCGCTCGACGTATGGAAGAGCTATCAGAGCCTG
CAGACAGACACCGCGAATCTCGACAACTCGAAGGACCTGCTCGACGATGCGCAGCGCTCGCTCGACATCGCGCGCGGCCG
CTACAAGGCGGGGGTCGGCACGTTCACGGAGCTGCTGAATGCGCAAACGGCGCTCTCCGACGCGCAGAAGCAGCGCGTGC
AGGCGATATCGAAATGGCGGACTGCGCGCCTGAAGCTCGCGGCGAGCCTCGGCAGCATCGGCCTGTGGTCGGCGCATTGA

Upstream 100 bases:

>100_bases
CGCCTGCTCCCGCCGCGGCACAGGCGGCGCCCGCTCCGGCCTGCGCGGCTCCCGCCGCGCCGCCCGCCGCTACGCAACCG
GCCACGGGGCGATGATCGTG

Downstream 100 bases:

>100_bases
AAGAACGTGAACGATGCGGCGGTGCGTTCTCGCTCCGGATGACGCGGCGATTGCCGAACGCGGCGACGCGCGCCGCGATT
CGGCACCGGCGGAAAAAACG

Product: TolC family type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKPARILAMAGALALFASQATHAQWLDLYGTRKNVSDSPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRAWAD
ARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQNLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQAREL
LAAANATQDDTLQTILFNAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVS
AEGDVQNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQGRPT
ISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSL
QTDTANLDNSKDLLDDAQRSLDIARGRYKAGVGTFTELLNAQTALSDAQKQRVQAISKWRTARLKLAASLGSIGLWSAH

Sequences:

>Translated_479_residues
MKPARILAMAGALALFASQATHAQWLDLYGTRKNVSDSPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRAWAD
ARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQNLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQAREL
LAAANATQDDTLQTILFNAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVS
AEGDVQNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQGRPT
ISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSL
QTDTANLDNSKDLLDDAQRSLDIARGRYKAGVGTFTELLNAQTALSDAQKQRVQAISKWRTARLKLAASLGSIGLWSAH
>Mature_479_residues
MKPARILAMAGALALFASQATHAQWLDLYGTRKNVSDSPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRAWAD
ARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQNLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQAREL
LAAANATQDDTLQTILFNAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVS
AEGDVQNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQGRPT
ISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSL
QTDTANLDNSKDLLDDAQRSLDIARGRYKAGVGTFTELLNAQTALSDAQKQRVQAISKWRTARLKLAASLGSIGLWSAH

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=423, Percent_Identity=21.7494089834515, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51431; Mature: 51431

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPARILAMAGALALFASQATHAQWLDLYGTRKNVSDSPAAPMLKNATCQPELADRPIEL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCH
EDAILQAICANPQARRAWADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQNLGP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCC
ITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQDDTLQTILFNAA
EEEECCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
AEGDVQNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAAR
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
AKLDAARANVDAVRAQGRPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFE
HHHHHHHCCHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEEEEEHHHH
GFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDNSKDLLDDAQRS
HHHCCCEEEHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LDIARGRYKAGVGTFTELLNAQTALSDAQKQRVQAISKWRTARLKLAASLGSIGLWSAH
HHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKPARILAMAGALALFASQATHAQWLDLYGTRKNVSDSPAAPMLKNATCQPELADRPIEL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCH
EDAILQAICANPQARRAWADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQNLGP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCC
ITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQDDTLQTILFNAA
EEEECCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
AEGDVQNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAAR
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
AKLDAARANVDAVRAQGRPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFE
HHHHHHHCCHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEEEEEHHHH
GFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDNSKDLLDDAQRS
HHHCCCEEEHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LDIARGRYKAGVGTFTELLNAQTALSDAQKQRVQAISKWRTARLKLAASLGSIGLWSAH
HHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]