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The map label for this gene is hemL [H]

Identifier: 83719810

GI number: 83719810

Start: 1743009

End: 1744292

Strand: Reverse

Name: hemL [H]

Synonym: BTH_I1543

Alternate gene names: 83719810

Gene position: 1744292-1743009 (Counterclockwise)

Preceding gene: 83718904

Following gene: 83718892

Centisome position: 45.79

GC content: 70.17

Gene sequence:

>1284_bases
ATGTCGAACAATCAAACTCTCTTCGAACGCGCCCAGCGAACCATCCCGGGCGGCGTCAATTCGCCGGTGCGGGCATTCCG
CTCGGTCGGCGGCACGCCGCGCTTCGTCGCGCGCGCGCAAGGCGCATACTTCTGGGACGCGGACGGCAAGCGCTACATCG
ACTACATCGGCTCGTGGGGCCCGATGATCGTCGGCCACGTGCACCCGGACGTGCTCGCCGCCGTGCAGCGCGTGCTCGCC
GACGGCTTCTCGTTCGGCGCGCCGACCGAAGCCGAAATCGAGATCGCCGAGGAAATCTGCAAGCTCGTGCCGTCGATCGA
GCAGGTGCGGATGGTGTCGAGCGGCACCGAAGCGACGATGAGCGCGCTGCGCCTCGCGCGCGGCTTCACCGGCCGCAGCC
GGATCGTCAAGTTCGAGGGCTGCTATCACGGCCATGCGGACAGCCTGCTCGTGAAGGCGGGCTCCGGCCTCCTCACGTTC
GGCAATCCGACGTCGGCCGGCGTGCCCGCCGACGTCGCGAAGCACACGACCGTGCTCGAGTACAACAACGTCGCGGCGCT
CGAGGAAGCGTTCGCCGCGTTCGGCGACGAGATCGCCGCGGTGATCGTCGAGCCCGTCGCGGGCAACATGAACCTCGTGC
GCGGCACGCCCGAATTCCTGAACGCGCTGCGCGCGCTCTGCACGAAGCACGGCGCCGTGCTGATCTTCGACGAAGTGATG
TGCGGCTTTCGCGTCGCGCTCGGCGGCGCGCAGCAGCACTACGGGATCAAGCCGGACCTGACCTGCCTCGGCAAGGTGAT
CGGCGGCGGCATGCCGGCCGCCGCGTTCGGCGGCCGCGGCGACATCATGTCGCACCTCGCGCCGCTTGGCGGCGTCTACC
AGGCCGGAACGCTGTCGGGCAACCCGGTCGCGGTCGCGGCGGGCCTGGCGACGCTCAGACTGATCCAGGCGCCGGGCTTT
CACGACGCGCTCGCCGACAAGACCCGCCGGCTCGCCGACGGCCTCGCGGCCGAGGCGCGCGCGGCGGGCGTGCCGTTCTC
GGCCGACGCGATCGGCGGAATGTTCGGCCTCTACTTCACCGAGCAGGTGCCCGCGAGCTTCGCAGACGTGACGAAGAGCG
ACATCGAGCGCTTCAACCGCTTCTTCCATCTGATGCTCGATGCCGGCGTGTACTTCGCGCCGTCCGCGTACGAAGCGGGC
TTCGTGTCGAGCGCGCACGACGACGCGACGCTCGACGCGACGCTCGACGCCGCGCGCCGCGCGTTCGCGGCGCTGCGCGC
CTGA

Upstream 100 bases:

>100_bases
AACCCGCTCCTCGCGTCTCCGGTACAATCGACCGATCGATACGCCCGACGCGAGCCGCGGCGCTTCGCCGCTCGCGCGCA
CTCTCTCGCAGGTCCAATTC

Downstream 100 bases:

>100_bases
CCTGCCGCACGACTGACAGGACGAACCGATGTTCTCGGAAAACGACTTCACCCACATGCAGCGCGCGCTCGCGCTCGCCG
CGCGCGGGATGTACACGACG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSNNQTLFERAQRTIPGGVNSPVRAFRSVGGTPRFVARAQGAYFWDADGKRYIDYIGSWGPMIVGHVHPDVLAAVQRVLA
DGFSFGAPTEAEIEIAEEICKLVPSIEQVRMVSSGTEATMSALRLARGFTGRSRIVKFEGCYHGHADSLLVKAGSGLLTF
GNPTSAGVPADVAKHTTVLEYNNVAALEEAFAAFGDEIAAVIVEPVAGNMNLVRGTPEFLNALRALCTKHGAVLIFDEVM
CGFRVALGGAQQHYGIKPDLTCLGKVIGGGMPAAAFGGRGDIMSHLAPLGGVYQAGTLSGNPVAVAAGLATLRLIQAPGF
HDALADKTRRLADGLAAEARAAGVPFSADAIGGMFGLYFTEQVPASFADVTKSDIERFNRFFHLMLDAGVYFAPSAYEAG
FVSSAHDDATLDATLDAARRAFAALRA

Sequences:

>Translated_427_residues
MSNNQTLFERAQRTIPGGVNSPVRAFRSVGGTPRFVARAQGAYFWDADGKRYIDYIGSWGPMIVGHVHPDVLAAVQRVLA
DGFSFGAPTEAEIEIAEEICKLVPSIEQVRMVSSGTEATMSALRLARGFTGRSRIVKFEGCYHGHADSLLVKAGSGLLTF
GNPTSAGVPADVAKHTTVLEYNNVAALEEAFAAFGDEIAAVIVEPVAGNMNLVRGTPEFLNALRALCTKHGAVLIFDEVM
CGFRVALGGAQQHYGIKPDLTCLGKVIGGGMPAAAFGGRGDIMSHLAPLGGVYQAGTLSGNPVAVAAGLATLRLIQAPGF
HDALADKTRRLADGLAAEARAAGVPFSADAIGGMFGLYFTEQVPASFADVTKSDIERFNRFFHLMLDAGVYFAPSAYEAG
FVSSAHDDATLDATLDAARRAFAALRA
>Mature_426_residues
SNNQTLFERAQRTIPGGVNSPVRAFRSVGGTPRFVARAQGAYFWDADGKRYIDYIGSWGPMIVGHVHPDVLAAVQRVLAD
GFSFGAPTEAEIEIAEEICKLVPSIEQVRMVSSGTEATMSALRLARGFTGRSRIVKFEGCYHGHADSLLVKAGSGLLTFG
NPTSAGVPADVAKHTTVLEYNNVAALEEAFAAFGDEIAAVIVEPVAGNMNLVRGTPEFLNALRALCTKHGAVLIFDEVMC
GFRVALGGAQQHYGIKPDLTCLGKVIGGGMPAAAFGGRGDIMSHLAPLGGVYQAGTLSGNPVAVAAGLATLRLIQAPGFH
DALADKTRRLADGLAAEARAAGVPFSADAIGGMFGLYFTEQVPASFADVTKSDIERFNRFFHLMLDAGVYFAPSAYEAGF
VSSAHDDATLDATLDAARRAFAALRA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=303, Percent_Identity=29.042904290429, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI284507298, Length=220, Percent_Identity=29.0909090909091, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=61.0328638497653, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1789016, Length=412, Percent_Identity=31.3106796116505, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789759, Length=311, Percent_Identity=30.2250803858521, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787560, Length=359, Percent_Identity=31.4763231197772, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788044, Length=330, Percent_Identity=30.9090909090909, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693181, Length=341, Percent_Identity=31.0850439882698, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1786991, Length=382, Percent_Identity=28.7958115183246, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=363, Percent_Identity=26.1707988980716, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=366, Percent_Identity=25.9562841530055, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32564660, Length=322, Percent_Identity=25.4658385093168, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144274, Length=210, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=335, Percent_Identity=28.0597014925373, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=350, Percent_Identity=25.1428571428571, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357415, Length=320, Percent_Identity=26.5625, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21356575, Length=348, Percent_Identity=27.0114942528736, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 44916; Mature: 44784

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNQTLFERAQRTIPGGVNSPVRAFRSVGGTPRFVARAQGAYFWDADGKRYIDYIGSWG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHCCCCEEECCCCCHHHHHHCCCC
PMIVGHVHPDVLAAVQRVLADGFSFGAPTEAEIEIAEEICKLVPSIEQVRMVSSGTEATM
CEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SALRLARGFTGRSRIVKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPADVAKHTTVLE
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHHCCEEEE
YNNVAALEEAFAAFGDEIAAVIVEPVAGNMNLVRGTPEFLNALRALCTKHGAVLIFDEVM
ECCHHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCHHHHHHHHHHHHHCCCEEEEHHHH
CGFRVALGGAQQHYGIKPDLTCLGKVIGGGMPAAAFGGRGDIMSHLAPLGGVYQAGTLSG
HHHHHHHCCCHHCCCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCCEECCCCCC
NPVAVAAGLATLRLIQAPGFHDALADKTRRLADGLAAEARAAGVPFSADAIGGMFGLYFT
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EQVPASFADVTKSDIERFNRFFHLMLDAGVYFAPSAYEAGFVSSAHDDATLDATLDAARR
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
AFAALRA
HHHHHCC
>Mature Secondary Structure 
SNNQTLFERAQRTIPGGVNSPVRAFRSVGGTPRFVARAQGAYFWDADGKRYIDYIGSWG
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHCCCCEEECCCCCHHHHHHCCCC
PMIVGHVHPDVLAAVQRVLADGFSFGAPTEAEIEIAEEICKLVPSIEQVRMVSSGTEATM
CEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SALRLARGFTGRSRIVKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPADVAKHTTVLE
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHHCCEEEE
YNNVAALEEAFAAFGDEIAAVIVEPVAGNMNLVRGTPEFLNALRALCTKHGAVLIFDEVM
ECCHHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCHHHHHHHHHHHHHCCCEEEEHHHH
CGFRVALGGAQQHYGIKPDLTCLGKVIGGGMPAAAFGGRGDIMSHLAPLGGVYQAGTLSG
HHHHHHHCCCHHCCCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCCEECCCCCC
NPVAVAAGLATLRLIQAPGFHDALADKTRRLADGLAAEARAAGVPFSADAIGGMFGLYFT
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EQVPASFADVTKSDIERFNRFFHLMLDAGVYFAPSAYEAGFVSSAHDDATLDATLDAARR
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
AFAALRA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA