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The map label for this gene is ytzG [H]

Identifier: 83719779

GI number: 83719779

Start: 911504

End: 912262

Strand: Reverse

Name: ytzG [H]

Synonym: BTH_I0795

Alternate gene names: 83719779

Gene position: 912262-911504 (Counterclockwise)

Preceding gene: 83718868

Following gene: 83721590

Centisome position: 23.95

GC content: 69.17

Gene sequence:

>759_bases
TTGCGCGTTCGATGCGCTTGGTACAATGCGCGCGCCATGGATCTCGAAAGCATTCTGTTCACGCAAGGCTTCGGGTCGCG
CCGCCAATGCCGCGCGCTCGTCGAAGCGGGACGCGTCGCCGTCGGCGGCGCGACGTGCGCCGACGCGCACACGTCATTCG
ACACGGCCCGGCTCGTGTTCGAGGTCGACGGTGTCGCGTGGCCGTACCGCGCGCGCGCGTACGTCGCGCTCAACAAGCCG
GCGGGCTACGAGTGCTCGCGCGAGCCGCAGCATCATGCGAGCGTGTTCAGCCTGCTGCCGCCGCAGTTCGCCGAGCGCGG
CGTCCAATGCGTCGGCCGCCTCGATCAGGACACGACGGGCCTGCTGCTGCTGTCCGACGACGGCCAGTTCGTCCACGCGT
ACACATCGCCGAAACGCAAGGTGCCGAAGACCTACGTCGCGACGGTGCGCCACCCGCTCGACGACGCGCAGCTCGACGCG
CTGCGCGCGGGCGTGCTGCTTCACGGCGAGCCGAAGCCGATCGCCGCGCTCGCCGCCGCCGCCCGAGGAGAACGACTCCT
CGAAATGACGATCGCCGAAGGGAAATACCACCAGGTGAAGCGGATGGTCGCCGCCGCGAGCAATCGCGTCGACGCGTTGC
ATCGCTCGCGCATCGGCGGCTTCGCGCTGCCCGAGGATCTCGCCGAAGGCGCCTGGCGCTGGCTCGACGAGCGCGATCTC
GCCGCATTGCGCGACACGGCAAAAACCCTATCAGGGTAA

Upstream 100 bases:

>100_bases
TTCGAACGAGCAATGGCGGTCGCGAGCGCGCGACCGCAGGCCCCGAGCTTAACAGCCCTCGCGCGGGCCTGCGCTCGGCC
GCGCGCCGGCCGTTCGCCCG

Downstream 100 bases:

>100_bases
GCGAGAAGGGCCGCCACTCGGCTTCCCCAGCCATGCTGCCGCGCAGCATGGCAGCGTCATCAAGCCCCGCTATACTCGAA
GCAAACCACAATACATTCCG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRVRCAWYNARAMDLESILFTQGFGSRRQCRALVEAGRVAVGGATCADAHTSFDTARLVFEVDGVAWPYRARAYVALNKP
AGYECSREPQHHASVFSLLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDA
LRAGVLLHGEPKPIAALAAAARGERLLEMTIAEGKYHQVKRMVAAASNRVDALHRSRIGGFALPEDLAEGAWRWLDERDL
AALRDTAKTLSG

Sequences:

>Translated_252_residues
MRVRCAWYNARAMDLESILFTQGFGSRRQCRALVEAGRVAVGGATCADAHTSFDTARLVFEVDGVAWPYRARAYVALNKP
AGYECSREPQHHASVFSLLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDA
LRAGVLLHGEPKPIAALAAAARGERLLEMTIAEGKYHQVKRMVAAASNRVDALHRSRIGGFALPEDLAEGAWRWLDERDL
AALRDTAKTLSG
>Mature_252_residues
MRVRCAWYNARAMDLESILFTQGFGSRRQCRALVEAGRVAVGGATCADAHTSFDTARLVFEVDGVAWPYRARAYVALNKP
AGYECSREPQHHASVFSLLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDA
LRAGVLLHGEPKPIAALAAAARGERLLEMTIAEGKYHQVKRMVAAASNRVDALHRSRIGGFALPEDLAEGAWRWLDERDL
AALRDTAKTLSG

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=170, Percent_Identity=42.9411764705882, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787524, Length=240, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=27.3504273504274, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27653; Mature: 27653

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRCAWYNARAMDLESILFTQGFGSRRQCRALVEAGRVAVGGATCADAHTSFDTARLVF
CEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCEEEEE
EVDGVAWPYRARAYVALNKPAGYECSREPQHHASVFSLLPPQFAERGVQCVGRLDQDTTG
EECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCE
LLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPKPIAALAAA
EEEECCCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCEEEECCCCHHHHHHHH
ARGERLLEMTIAEGKYHQVKRMVAAASNRVDALHRSRIGGFALPEDLAEGAWRWLDERDL
HCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHH
AALRDTAKTLSG
HHHHHHHHHHCC
>Mature Secondary Structure
MRVRCAWYNARAMDLESILFTQGFGSRRQCRALVEAGRVAVGGATCADAHTSFDTARLVF
CEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCEEEEE
EVDGVAWPYRARAYVALNKPAGYECSREPQHHASVFSLLPPQFAERGVQCVGRLDQDTTG
EECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCE
LLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPKPIAALAAA
EEEECCCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCEEEECCCCHHHHHHHH
ARGERLLEMTIAEGKYHQVKRMVAAASNRVDALHRSRIGGFALPEDLAEGAWRWLDERDL
HCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHH
AALRDTAKTLSG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]