Click here to switch to the map view.

The map label for this gene is nirB-1 [H]

Identifier: 83719776

GI number: 83719776

Start: 517846

End: 520290

Strand: Reverse

Name: nirB-1 [H]

Synonym: BTH_I0464

Alternate gene names: 83719776

Gene position: 520290-517846 (Counterclockwise)

Preceding gene: 83718719

Following gene: 83718936

Centisome position: 13.66

GC content: 67.24

Gene sequence:

>2445_bases
ATGCGGAAGATGAAGCTGGTGATGGTCGGCAACGGCATGGCCGGAGTGCGCACGCTCGAAGAATTGCTCAAGCTCGCGCC
GGATCTGTACGACGTGACGGTGTTCGGCGCCGAACCGCATCCGAACTACAACCGGATCCTGCTATCCCCCGTGCTCGCGG
GCGAGCAGACGCTCGAGCAGATCGTGCTCAACGATTATGCGTGGTACGAATCGCACGGCATCACGCTCCACGTCGGCAAG
AAGATCGTGAAGATCGATCGCGTGAAGCGCGTCGTGATCGCCGACGACGGCACCGAGGCCGCGTACGACCGGCTGCTGCT
CGCAACCGGCTCGAATCCGTTCATGCTGCCGATTCCGGGCGCGGATCTCGACGGCGTGCTCGGCTATCGCGACATCGCGG
ACACGCAGGCGATGATCGACGCCGCCGCGCGGCACACGCACGCGGTCGTGATCGGCGGCGGCCTGCTCGGCCTCGAGGCG
GCGAACGGCCTGAAGCTGCGCGGGATGGACGTGACCGTCGCGCACCTCGCGCCGACGCTGCTCGAGCGCCAGCTCGACGC
GAAGGCGGGCGGCCTGTTGCGCGCGTCGCTCGAGGCGCGCGGGCTGACCTTTCTGATGCCGAAGGAAACGCAGGCGCTCG
TCGGCGACGAGAACGGCCGCGTGCGCGCGGTGCGCTTCAAGGACGGCGACACATGCAAGGCCGACCTCGTCGTGATGGCG
GTCGGCATCCGCCCGAACACCGCGCTCGCCGAAAGCGCGGGCCTCTATTGCAACCGCGGGATCGTCGTCAACGACACGAT
GCAAACCTACGATCCGCGCATCTACGCGGTCGGCGAATGCGTGAGCCATCGCGGCATCGCGTACGGCCTCGTCGCGCCCC
TCTTCGAGCAGGCGAAGGTCGCGGCGAACCATCTCGCGCAATTCGGCATCGGCCGCTACGCGGGCTCGGTGACGTCGACG
AAGCTCAAGGTCACGGGCATCGACCTGTTCTCCGCGGGCGACTTCCTCGGCGGCGGCGACACCGAGGACATCACGCTGTC
CGATCCGATCGCGGGCGTCTACAAGAAGCTCGTGATCAAGGACGACAAGATCGTCGGCGCGTGCCTGTACGGCGATACGG
CAGACGGCGCGTGGTACTTCAAGCTGCTGCGCGAGGGCCGCAACATCGCCGACATCCGCGAGACGCTGATGTTCGGCGAG
ACGAGCCTGGGCGACACTGGCCACAGCGGCGCGACGCGCGCGACGGCGATGGCCGACGACGCCGAGGTGTGCGGCTGCAA
CGGCGTGTGCAAGGGCACGATCGTCGCGGCGATCAAGGAGAAAGGCCTCTTCACGCTCGACGACGTGCGCAAGCACACGA
AGGCGTCCGCATCGTGCGGCTCGTGCACGGGCCTCGTCGAGCAGATCCTGATGTCGACGGTGGGCGGCGACTACTCGGCG
TCGCCGAAGGCGAAGCCCATATGCGGCTGCACCGATCATACGCATGCCGAGGTGCGCATGGCGATTCGCGAGCACAAGCT
GCTGTCGGTGCCGGACGCGATGCGCTTTCTCGAATGGCGCACGCCGAACGGCTGCGCGAGCTGCCGCCCGGCGCTCAACT
ACTACTGCATCAGCACGTGGCCGCACGACGCGAAGGACGATCCGCAGTCGCGCTTCATCAACGAGCGCGCGCACGCGAAC
ATCCAGAAGGACGGCACGTATTCGGTCGTGCCGCGGATGTGGGGCGGCGTGACGAGCGCCGACGAACTGCGCCGCATCGC
CGACGTCGTCGACAAATACGCGATCCCCACCGTCAAGGTGACGGGCGGCCAGCGCATCGACCTGCTCGGCGTGAAGAAAG
AGGACCTGCCCGGCGTGTGGCGCGATCTCGGCATGCCGAGCGGCCATGCGTACGCGAAAGCGCTGCGCACGGTGAAGACC
TGCGTCGGCTCCGAATGGTGCCGCTTCGGCACGCAGGATTCGACGCTGATGGGCCAGCAGCTCGAGCGCGCGCTGTGGCG
GATGTACGCGCCGCACAAGGTGAAGCTCGCTGTGTCCGGCTGCCCGCGCAACTGCGCGGAATCGGGGATCAAGGATGTCG
GCGTGATCGGCGTGGATTCGGGCTGGGAGCTTTACGTCGGCGGCAACGGCGGCATCAAGACCGAGGTCGCGCAGTTCTTC
TGCAAGGTGAAGACGCACGAGGAAGTGCTCGAATATGCAGGCGCGTTCCTGCAGCTGTATCGCGAGGAAGGCTGGTATCT
CGAGCGCACCGTGCACTACCTCGAGCGCGTCGGCCTCGACCACGTGAAGGCGCGCGTGCTCGACGACGCCGAGAACCGCC
GCGCGCTGTGGGAACGGCTGCAGTTCGCGCTGAAGGACGAGCCGGACCCGTGGCGCGACACGCAGGACGCGAAGGTCGAC
CTGCGCCAGTTCATCCCGATCGCCGTCGCGCCCGCCGATGCGTGA

Upstream 100 bases:

>100_bases
TTTTAAACAGCGGCCAAATAAAAAGTGGCATGGCAATTGCTGTTTTCCGCCCATCGAACACGGGCCCCACACGACAAGCG
GCCCAGTCAGGAGATCCCCG

Downstream 100 bases:

>100_bases
TGCCGATGCGCGATGCGTGACGCGTCGCGCATCGCGCCGCCCGCGCGCCCCGTCAATCACCCAGGAATCCGAATCATGTC
CGCCACGCAACCCGAATGGA

Product: nitrite reductase [NAD(P)H] large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MRKMKLVMVGNGMAGVRTLEELLKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQIVLNDYAWYESHGITLHVGK
KIVKIDRVKRVVIADDGTEAAYDRLLLATGSNPFMLPIPGADLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEA
ANGLKLRGMDVTVAHLAPTLLERQLDAKAGGLLRASLEARGLTFLMPKETQALVGDENGRVRAVRFKDGDTCKADLVVMA
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYAGSVTST
KLKVTGIDLFSAGDFLGGGDTEDITLSDPIAGVYKKLVIKDDKIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGE
TSLGDTGHSGATRATAMADDAEVCGCNGVCKGTIVAAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTVGGDYSA
SPKAKPICGCTDHTHAEVRMAIREHKLLSVPDAMRFLEWRTPNGCASCRPALNYYCISTWPHDAKDDPQSRFINERAHAN
IQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKT
CVGSEWCRFGTQDSTLMGQQLERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWELYVGGNGGIKTEVAQFF
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWERLQFALKDEPDPWRDTQDAKVD
LRQFIPIAVAPADA

Sequences:

>Translated_814_residues
MRKMKLVMVGNGMAGVRTLEELLKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQIVLNDYAWYESHGITLHVGK
KIVKIDRVKRVVIADDGTEAAYDRLLLATGSNPFMLPIPGADLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEA
ANGLKLRGMDVTVAHLAPTLLERQLDAKAGGLLRASLEARGLTFLMPKETQALVGDENGRVRAVRFKDGDTCKADLVVMA
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYAGSVTST
KLKVTGIDLFSAGDFLGGGDTEDITLSDPIAGVYKKLVIKDDKIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGE
TSLGDTGHSGATRATAMADDAEVCGCNGVCKGTIVAAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTVGGDYSA
SPKAKPICGCTDHTHAEVRMAIREHKLLSVPDAMRFLEWRTPNGCASCRPALNYYCISTWPHDAKDDPQSRFINERAHAN
IQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKT
CVGSEWCRFGTQDSTLMGQQLERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWELYVGGNGGIKTEVAQFF
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWERLQFALKDEPDPWRDTQDAKVD
LRQFIPIAVAPADA
>Mature_814_residues
MRKMKLVMVGNGMAGVRTLEELLKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQIVLNDYAWYESHGITLHVGK
KIVKIDRVKRVVIADDGTEAAYDRLLLATGSNPFMLPIPGADLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEA
ANGLKLRGMDVTVAHLAPTLLERQLDAKAGGLLRASLEARGLTFLMPKETQALVGDENGRVRAVRFKDGDTCKADLVVMA
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYAGSVTST
KLKVTGIDLFSAGDFLGGGDTEDITLSDPIAGVYKKLVIKDDKIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGE
TSLGDTGHSGATRATAMADDAEVCGCNGVCKGTIVAAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTVGGDYSA
SPKAKPICGCTDHTHAEVRMAIREHKLLSVPDAMRFLEWRTPNGCASCRPALNYYCISTWPHDAKDDPQSRFINERAHAN
IQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKT
CVGSEWCRFGTQDSTLMGQQLERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWELYVGGNGGIKTEVAQFF
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWERLQFALKDEPDPWRDTQDAKVD
LRQFIPIAVAPADA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=280, Percent_Identity=30.3571428571429, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI226437568, Length=280, Percent_Identity=30.3571428571429, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI21389617, Length=280, Percent_Identity=30.3571428571429, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4757732, Length=237, Percent_Identity=27.8481012658228, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI22202629, Length=237, Percent_Identity=27.8481012658228, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=832, Percent_Identity=36.1778846153846, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1789065, Length=301, Percent_Identity=28.2392026578073, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788892, Length=288, Percent_Identity=27.0833333333333, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=401, Percent_Identity=25.6857855361596, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281359715, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281359713, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24639250, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18543267, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24639252, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24639257, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585130, Length=319, Percent_Identity=23.1974921630094, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573993, Length=216, Percent_Identity=30.0925925925926, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24581020, Length=263, Percent_Identity=28.8973384030418, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88617; Mature: 88617

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMKLVMVGNGMAGVRTLEELLKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQ
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEHHHCCHHHHHH
IVLNDYAWYESHGITLHVGKKIVKIDRVKRVVIADDGTEAAYDRLLLATGSNPFMLPIPG
HHHHCCCEEECCCEEEECCCHHHEEHHEEEEEEECCCCHHHHCEEEEEECCCCEEEEECC
ADLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEAANGLKLRGMDVTVAHLAPTL
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCEEEECCCEEHHHHHHHH
LERQLDAKAGGLLRASLEARGLTFLMPKETQALVGDENGRVRAVRFKDGDTCKADLVVMA
HHHHHCCCCCCEEEEEHHHCCEEEECCCCCHHEECCCCCCEEEEEECCCCCCCEEEEEEE
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKV
ECCCCCCHHHHCCCCEECCCEEECCCHHHCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHH
AANHLAQFGIGRYAGSVTSTKLKVTGIDLFSAGDFLGGGDTEDITLSDPIAGVYKKLVIK
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHCC
DDKIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGETSLGDTGHSGATRATAMADD
CCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCC
AEVCGCNGVCKGTIVAAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTVGGDYSA
CCCCCCCCCCCHHEEEEEHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SPKAKPICGCTDHTHAEVRMAIREHKLLSVPDAMRFLEWRTPNGCASCRPALNYYCISTW
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECC
PHDAKDDPQSRFINERAHANIQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKV
CCCCCCCHHHHHHHHHHCCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
TGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTLMGQQ
CCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHH
LERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWELYVGGNGGIKTEVAQFF
HHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHH
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWERL
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHH
QFALKDEPDPWRDTQDAKVDLRQFIPIAVAPADA
HHHHCCCCCCCCCCCCHHHHHHHCCCEEEECCCC
>Mature Secondary Structure
MRKMKLVMVGNGMAGVRTLEELLKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQ
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEHHHCCHHHHHH
IVLNDYAWYESHGITLHVGKKIVKIDRVKRVVIADDGTEAAYDRLLLATGSNPFMLPIPG
HHHHCCCEEECCCEEEECCCHHHEEHHEEEEEEECCCCHHHHCEEEEEECCCCEEEEECC
ADLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEAANGLKLRGMDVTVAHLAPTL
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCEEEECCCEEHHHHHHHH
LERQLDAKAGGLLRASLEARGLTFLMPKETQALVGDENGRVRAVRFKDGDTCKADLVVMA
HHHHHCCCCCCEEEEEHHHCCEEEECCCCCHHEECCCCCCEEEEEECCCCCCCEEEEEEE
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKV
ECCCCCCHHHHCCCCEECCCEEECCCHHHCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHH
AANHLAQFGIGRYAGSVTSTKLKVTGIDLFSAGDFLGGGDTEDITLSDPIAGVYKKLVIK
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHCC
DDKIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGETSLGDTGHSGATRATAMADD
CCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCC
AEVCGCNGVCKGTIVAAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTVGGDYSA
CCCCCCCCCCCHHEEEEEHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SPKAKPICGCTDHTHAEVRMAIREHKLLSVPDAMRFLEWRTPNGCASCRPALNYYCISTW
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECC
PHDAKDDPQSRFINERAHANIQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKV
CCCCCCCHHHHHHHHHHCCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
TGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTLMGQQ
CCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHH
LERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWELYVGGNGGIKTEVAQFF
HHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHH
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWERL
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHH
QFALKDEPDPWRDTQDAKVDLRQFIPIAVAPADA
HHHHCCCCCCCCCCCCHHHHHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]