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The map label for this gene is potF [H]

Identifier: 83719634

GI number: 83719634

Start: 3669753

End: 3670103

Strand: Direct

Name: potF [H]

Synonym: BTH_I3223

Alternate gene names: 83719634

Gene position: 3669753-3670103 (Clockwise)

Preceding gene: 83720742

Following gene: 83719866

Centisome position: 96.34

GC content: 54.99

Gene sequence:

>351_bases
GTGCGTGCACAGGAACGCCTCCCGAGCATCAAGTTCGTCTATCCAAAGGAGGGCGTTCGCCTGTTCATGGACAGTCTGCT
CATCCCCGCAAAAGCTCGCAATATCGATTCCGCCTACAAGTTCCTTAATTGGATGATGCAACCGGAGAACATCGCGCTGG
TGACGAATGCGGTTCGGTACAACAACGAGATCATCGGTTCCGAACGCTACGTAGATGCAGCGTTGTTGGACAACCCGGCA
GTCAAGACGCCAGAGCAGTACAAGGCACGGCTCAGGCCGTACAAGCTGTGCTCGCTGGCCGCGATTCAGTTACGCAACAA
GGTCTGGCTGAAGCTGAAGGGGAATCGATAA

Upstream 100 bases:

>100_bases
TCGTAAAATGCACCGATGTCACGCTCTCTCATAGGACGGTACGGTCGATCGTCTCGCGTCCCGCCAAATCGCCGTTCAAC
ATGTCTGGAACGGCGCCGCA

Downstream 100 bases:

>100_bases
GCTACGTTGCCGATCACGGCTTCCCCTGAAGCGATTTGAAGAGAAGCGGCGGCGGGCGATGCTGCGAACATCGCCCGCCG
CCTAACCGAGGTGCACACAT

Product: ABC transporter

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MRAQERLPSIKFVYPKEGVRLFMDSLLIPAKARNIDSAYKFLNWMMQPENIALVTNAVRYNNEIIGSERYVDAALLDNPA
VKTPEQYKARLRPYKLCSLAAIQLRNKVWLKLKGNR

Sequences:

>Translated_116_residues
MRAQERLPSIKFVYPKEGVRLFMDSLLIPAKARNIDSAYKFLNWMMQPENIALVTNAVRYNNEIIGSERYVDAALLDNPA
VKTPEQYKARLRPYKLCSLAAIQLRNKVWLKLKGNR
>Mature_116_residues
MRAQERLPSIKFVYPKEGVRLFMDSLLIPAKARNIDSAYKFLNWMMQPENIALVTNAVRYNNEIIGSERYVDAALLDNPA
VKTPEQYKARLRPYKLCSLAAIQLRNKVWLKLKGNR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=108, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 13469; Mature: 13469

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQERLPSIKFVYPKEGVRLFMDSLLIPAKARNIDSAYKFLNWMMQPENIALVTNAVRY
CCCHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHC
NNEIIGSERYVDAALLDNPAVKTPEQYKARLRPYKLCSLAAIQLRNKVWLKLKGNR
CCEECCCCCHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCC
>Mature Secondary Structure
MRAQERLPSIKFVYPKEGVRLFMDSLLIPAKARNIDSAYKFLNWMMQPENIALVTNAVRY
CCCHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHC
NNEIIGSERYVDAALLDNPAVKTPEQYKARLRPYKLCSLAAIQLRNKVWLKLKGNR
CCEECCCCCHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]