Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 83719624

GI number: 83719624

Start: 674640

End: 676013

Strand: Reverse

Name: miaB [H]

Synonym: BTH_I0591

Alternate gene names: 83719624

Gene position: 676013-674640 (Counterclockwise)

Preceding gene: 83718916

Following gene: 83720402

Centisome position: 17.75

GC content: 65.65

Gene sequence:

>1374_bases
ATGACCAAGAAAGTTTACGTAAAAACCTTCGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGTCGACGTGCTCAA
CGCCGCCGAAGGGCTGGAAAAGACCGACAGCCCGGAAGACGCGGACATCATCCTGTTCAACACCTGCTCGGTGCGCGAAA
AGGCCCAGGAGAAGGTGTTCTCCGATCTCGGACGCGTGCGCGAGCTCAAGGAGGCGAAGCCGGATCTGCTGATCGGCGTC
GGCGGCTGCGTCGCGAGCCAGGAAGGCGCGTCGATCGTCGCGCGCGCGCCGTACGTCGACCTCGTGTTCGGCCCGCAGAC
GCTGCACCGCCTGCCGCAGATGATCGACGCGCGCCGCGAAAGCGGCCGCGCGCAGGTCGACATCACGTTCCCCGAAATCG
AGAAGTTCGACCACCTGCCGCCCGCGCGCGTCGAAGGCCCGAGCGCGTTCGTGTCGATCATGGAAGGCTGCTCGAAATAC
TGCAGCTACTGCGTCGTGCCGTACACGCGCGGCGACGAAGTCTCGCGCCCGCTCGACGACGTGCTGACCGAGATCGCGGG
CCTCGCCGATCAGGGCGTGCGCGAAGTCACGCTGCTCGGCCAGAACGTGAACGCATACCGGGGCGCGCTGACGGCGGGCG
CGCACGAGATCGCCGATTTCGCGACGCTCATCGAATACGTCGCCGACATCCCCGGCATCGAGCGGATCCGCTACACGACG
TCGCATCCGAAGGAATTCACGCAGCGCCTGCTCGACGTGTACGCGAAGGTGCCGAAGCTCGTCGACCATCTGCACCTGCC
CGTGCAGCACGGCTCGGACCGCATTCTGATGGCGATGAAGCGCGGCTACACGGTGCTCGAATACAAATCGCTGATCCGCA
AGCTGCGCGCGATCCGCCCGAACCTGTCGCTGTCGACGGACATCATCGTGGGCTTCCCCGGCGAGACCGAGGCCGACTTC
GACAAGACGATGGCGCTCGTTCACGAGATGAGCTACGACACGAGCTTCTCGTTCATCTACAGCCCGCGCCCCGGCACGCC
GGCCGCGAACCTCGCCGACGACACGCCGCGCGAGGTCAAGCTCGAGCGCCTGCAACATCTGCAGGCGACGATCGAGGAGA
ACGTCGCGAGGATCAGCCAGTCGATGCTCGGCAAGGTCGAGCGAATCCTCGTCGAGGGGCCGTCGCGCAAGGACCCGAAC
GAGCTCGCAGGCCGCACCGAGAACAACCGGGTCGTGAATTTCCCCGCGCCGCTCACGGCGCACGCGCGCCTGATCGGCCA
GATGATCGACGTGAAGATCAATCACGCGTATCCGCACTCGCTGCGCGGCGAGCTCGTGCTCGCGCACGACGACGCGAGCG
CCGCCACGCACTGA

Upstream 100 bases:

>100_bases
ATGCGGCGCGTGCATCGCGCGGCTGGCGGCGCGCCGGTCCGTCCCCCTGATTCATTTTGAACGACGCCCCCAGGTCAACC
ACTGCGAACGGCGAATCCTC

Downstream 100 bases:

>100_bases
AGCTACCGGAACCCGACGCCACTTTGAAAACTGCACAAGCACTGGAATTCACCGCGCCGCGCGACGACAACGCGCGCCTC
GCGAACCTCTGCGGCCCGCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGV
GGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGALTAGAHEIADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRPNLSLSTDIIVGFPGETEADF
DKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPREVKLERLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPLTAHARLIGQMIDVKINHAYPHSLRGELVLAHDDASAATH

Sequences:

>Translated_457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGV
GGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGALTAGAHEIADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRPNLSLSTDIIVGFPGETEADF
DKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPREVKLERLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPLTAHARLIGQMIDVKINHAYPHSLRGELVLAHDDASAATH
>Mature_456_residues
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGVG
GCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGDEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGALTAGAHEIADFATLIEYVADIPGIERIRYTTS
HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRPNLSLSTDIIVGFPGETEADFD
KTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPREVKLERLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNE
LAGRTENNRVVNFPAPLTAHARLIGQMIDVKINHAYPHSLRGELVLAHDDASAATH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=36.9519832985386, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=36.9519832985386, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI93277076, Length=467, Percent_Identity=27.8372591006424, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=61.4190687361419, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=29.2841648590022, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=34.475374732334, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=29.3333333333333, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=33.8912133891213, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=28.5403050108932, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50695; Mature: 50564

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRE
HHHHHHHHHHHCCCCEEEECCCHHCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHH
SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEIAGLADQGVREVTLLGQNVNAYRGALTAGAHEIADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHCC
NLSLSTDIIVGFPGETEADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPREVK
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LERLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEECCCCHHH
HARLIGQMIDVKINHAYPHSLRGELVLAHDDASAATH
HHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRE
HHHHHHHHHHHCCCCEEEECCCHHCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHH
SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEIAGLADQGVREVTLLGQNVNAYRGALTAGAHEIADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHCC
NLSLSTDIIVGFPGETEADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPREVK
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LERLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEECCCCHHH
HARLIGQMIDVKINHAYPHSLRGELVLAHDDASAATH
HHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA