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The map label for this gene is mdlC [H]

Identifier: 83719574

GI number: 83719574

Start: 812105

End: 813724

Strand: Direct

Name: mdlC [H]

Synonym: BTH_I0705

Alternate gene names: 83719574

Gene position: 812105-813724 (Clockwise)

Preceding gene: 83718962

Following gene: 83720754

Centisome position: 21.32

GC content: 72.96

Gene sequence:

>1620_bases
ATGACCCCCCCCGATCTTCGCGCCGACGCGCCGGCGCACGCGCCGTACACGGTTCGCGACGCCGTAATCGACCTGCTGCG
CCGGTTCGGCATCCGCAAGGTGTTCGGCAATCCGGGCTCGACCGAGCTGCCGATGTTCCGCGATTTCCCCGCCGATTTCG
AATACGTGCTCGGCCTGCACGAGGCGGTCGCGGTGGGGATGGCGGACGGCTACGCGCAGGCGAGCGGCAACGCGGCCGTC
GTGAACCTGCATTCGGCGGCGGGCGTCGGCAACGCGATGGGCGTTCTGTTCACCGCGTACAAGAACAAGACGCCGCTCGT
CGTCACGGCCGGCCAGCAGGCGCGCTCGATCCTGCCGTTCGAGCCGTTTCTCGCGTCGGTGCAGGCGGCCGAATTGCCGA
AGCCGTACGTGAAATGGAGCATCGAGCCCGCGCGCGCCGAGGACGTGCCCGCCGCGATCGCCCGCGCGTACCACGTCGCG
ATGCAGGAGCCGCGCGGGCCCGTGTTCGTATCGATTCCGGCCGACGACTGGGATCGCCCCGCCGAGCCCATCGCCGCGCG
CACGGTGAGCAGCGAAGTGCGGCCCGCGCCCGATGCGCTCGCGCGATTGGGCGACGCGCTCGACGCGAGCGAGCGTCCGG
CGTTCGTGGTCGGCGCGGCGGTGGCGCGCGCGGGGGCGTGGGACGAAGCGGTGCGGCTCGCCGAGCGGCACCGCGCGCGC
GTGTACGCCGCGCCGATGTCGGGCCGCTGCAGCTTTCCCGAGGATCATCCGCTCTTCGCGGGCTTTCTGCCGCCGATGCG
CGAGCGGATCGTCGAACGGCTCGCCGGCCACGATTTCATTCTCGCGTTCGGCGCGCCCGTCTTCACGTATCACGTCGAAG
GCTTCGGCCCGCACGTGCCCGCGGGCGCGACGCTTCATCAGCTCGTCGACGATCCGTCGGTGGCCGCGTGGACGCCCGTC
GGCGACGCGGTCGTCGGCAATCTGCGGCTTGCCGCGCGCGACCTGCTCGCGCGTCCCGTGCCGCGCGAGCGGCCGATGCC
CGCGCCGCGCGCGCCGCACGAGCGCGCCGGCCCGGGCGCGCCGGGCGAGCGCATGCCGGTGGCGTTCGCGCTGCAGACGC
TCGCCGACGTGCGCGGCCCGGACGACATCGTCGTCGAGGAAGCGCCGAGCGCGCGGCCGGCGATGCAGCAGCACCTGCCG
TTCTCGCGCGTCGACACGTTTTACACGATGGACAGCGGCGGCCTCGGCTACGGGATGCCGGCCGCGGTCGGCGTCGCGCT
CGGCTCGCCGGGGCGGCGCGTGATCGCGCTGATCGGCGACGGCTCGAGCCTCTATTCGATTCAGGCGCTCTGGAGCGCCG
CGCACCTGAAATTGCCGGTCACCTTCGTGATCCTGAACAACGCGCGCTACGCGGCGCTGCAGGAGTTCGCGCCCGTGTTC
GGCTTTCGCGACGACGAGCCGGTGCAGGGCACCGCGCTGCCCGATCTCGATTTCGTCTCGCTCGCGCGCGGGCTCGGTTG
CGACGGCGTGCGCGTGAGCGACGCGGCGCGCCTGCGCGACGCGCTCGAACGCGCGCTCGCGTCGCCCGCGCCCGTCGTCG
TCGACGTGCAGATCGCGTGA

Upstream 100 bases:

>100_bases
GTCGTGTAATGGCTTATTGCAGAAAATACAGTATCGAGGAATGTGTGCGCCGCCGATACTGTGGAAACCAATGACGACGA
CGTTGAGGAGACACGCCCGC

Downstream 100 bases:

>100_bases
CGCGCGCGAGCCCCACGAGCCCCACGAACTGCGCTCGATGGCGTTCATGAGCGTCGCGAAGCGCGGCAAAGCACTCAGAA
GGAGAACACCCGCATGCAGG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTPPDLRADAPAHAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRDFPADFEYVLGLHEAVAVGMADGYAQASGNAAV
VNLHSAAGVGNAMGVLFTAYKNKTPLVVTAGQQARSILPFEPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVA
MQEPRGPVFVSIPADDWDRPAEPIAARTVSSEVRPAPDALARLGDALDASERPAFVVGAAVARAGAWDEAVRLAERHRAR
VYAAPMSGRCSFPEDHPLFAGFLPPMRERIVERLAGHDFILAFGAPVFTYHVEGFGPHVPAGATLHQLVDDPSVAAWTPV
GDAVVGNLRLAARDLLARPVPRERPMPAPRAPHERAGPGAPGERMPVAFALQTLADVRGPDDIVVEEAPSARPAMQQHLP
FSRVDTFYTMDSGGLGYGMPAAVGVALGSPGRRVIALIGDGSSLYSIQALWSAAHLKLPVTFVILNNARYAALQEFAPVF
GFRDDEPVQGTALPDLDFVSLARGLGCDGVRVSDAARLRDALERALASPAPVVVDVQIA

Sequences:

>Translated_539_residues
MTPPDLRADAPAHAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRDFPADFEYVLGLHEAVAVGMADGYAQASGNAAV
VNLHSAAGVGNAMGVLFTAYKNKTPLVVTAGQQARSILPFEPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVA
MQEPRGPVFVSIPADDWDRPAEPIAARTVSSEVRPAPDALARLGDALDASERPAFVVGAAVARAGAWDEAVRLAERHRAR
VYAAPMSGRCSFPEDHPLFAGFLPPMRERIVERLAGHDFILAFGAPVFTYHVEGFGPHVPAGATLHQLVDDPSVAAWTPV
GDAVVGNLRLAARDLLARPVPRERPMPAPRAPHERAGPGAPGERMPVAFALQTLADVRGPDDIVVEEAPSARPAMQQHLP
FSRVDTFYTMDSGGLGYGMPAAVGVALGSPGRRVIALIGDGSSLYSIQALWSAAHLKLPVTFVILNNARYAALQEFAPVF
GFRDDEPVQGTALPDLDFVSLARGLGCDGVRVSDAARLRDALERALASPAPVVVDVQIA
>Mature_538_residues
TPPDLRADAPAHAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRDFPADFEYVLGLHEAVAVGMADGYAQASGNAAVV
NLHSAAGVGNAMGVLFTAYKNKTPLVVTAGQQARSILPFEPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVAM
QEPRGPVFVSIPADDWDRPAEPIAARTVSSEVRPAPDALARLGDALDASERPAFVVGAAVARAGAWDEAVRLAERHRARV
YAAPMSGRCSFPEDHPLFAGFLPPMRERIVERLAGHDFILAFGAPVFTYHVEGFGPHVPAGATLHQLVDDPSVAAWTPVG
DAVVGNLRLAARDLLARPVPRERPMPAPRAPHERAGPGAPGERMPVAFALQTLADVRGPDDIVVEEAPSARPAMQQHLPF
SRVDTFYTMDSGGLGYGMPAAVGVALGSPGRRVIALIGDGSSLYSIQALWSAAHLKLPVTFVILNNARYAALQEFAPVFG
FRDDEPVQGTALPDLDFVSLARGLGCDGVRVSDAARLRDALERALASPAPVVVDVQIA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=534, Percent_Identity=26.7790262172285, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790104, Length=557, Percent_Identity=23.5188509874327, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1786717, Length=572, Percent_Identity=25.6993006993007, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=24.865831842576, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=23.613595706619, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=555, Percent_Identity=25.2252252252252, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 57387; Mature: 57256

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPDLRADAPAHAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRDFPADFEYVLGLH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
EAVAVGMADGYAQASGNAAVVNLHSAAGVGNAMGVLFTAYKNKTPLVVTAGQQARSILPF
HHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCHHHHEEEEECCCCCEEEECCHHHCCCCCC
EPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVAMQEPRGPVFVSIPADDWDRP
HHHHHHHHHHHCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
AEPIAARTVSSEVRPAPDALARLGDALDASERPAFVVGAAVARAGAWDEAVRLAERHRAR
CCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCE
VYAAPMSGRCSFPEDHPLFAGFLPPMRERIVERLAGHDFILAFGAPVFTYHVEGFGPHVP
EEECCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCC
AGATLHQLVDDPSVAAWTPVGDAVVGNLRLAARDLLARPVPRERPMPAPRAPHERAGPGA
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PGERMPVAFALQTLADVRGPDDIVVEEAPSARPAMQQHLPFSRVDTFYTMDSGGLGYGMP
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCHHHCCEEEEECCCCCCCCCH
AAVGVALGSPGRRVIALIGDGSSLYSIQALWSAAHLKLPVTFVILNNARYAALQEFAPVF
HHHHEEECCCCCEEEEEEECCCCHHHHHHHHHHHHEECEEEEEEECCCHHHHHHHHHHHH
GFRDDEPVQGTALPDLDFVSLARGLGCDGVRVSDAARLRDALERALASPAPVVVDVQIA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
TPPDLRADAPAHAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRDFPADFEYVLGLH
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
EAVAVGMADGYAQASGNAAVVNLHSAAGVGNAMGVLFTAYKNKTPLVVTAGQQARSILPF
HHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCHHHHEEEEECCCCCEEEECCHHHCCCCCC
EPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVAMQEPRGPVFVSIPADDWDRP
HHHHHHHHHHHCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
AEPIAARTVSSEVRPAPDALARLGDALDASERPAFVVGAAVARAGAWDEAVRLAERHRAR
CCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCE
VYAAPMSGRCSFPEDHPLFAGFLPPMRERIVERLAGHDFILAFGAPVFTYHVEGFGPHVP
EEECCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCC
AGATLHQLVDDPSVAAWTPVGDAVVGNLRLAARDLLARPVPRERPMPAPRAPHERAGPGA
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PGERMPVAFALQTLADVRGPDDIVVEEAPSARPAMQQHLPFSRVDTFYTMDSGGLGYGMP
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCHHHCCEEEEECCCCCCCCCH
AAVGVALGSPGRRVIALIGDGSSLYSIQALWSAAHLKLPVTFVILNNARYAALQEFAPVF
HHHHEEECCCCCEEEEEEECCCCHHHHHHHHHHHHEECEEEEEEECCCHHHHHHHHHHHH
GFRDDEPVQGTALPDLDFVSLARGLGCDGVRVSDAARLRDALERALASPAPVVVDVQIA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]