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The map label for this gene is thiC

Identifier: 83719533

GI number: 83719533

Start: 3261445

End: 3263391

Strand: Direct

Name: thiC

Synonym: BTH_I2844

Alternate gene names: 83719533

Gene position: 3261445-3263391 (Clockwise)

Preceding gene: 83721260

Following gene: 83720064

Centisome position: 85.62

GC content: 65.18

Gene sequence:

>1947_bases
ATGAACGCCAACCCCAAGTTCCTGTCGGCCGACGCCCGCGTCGACGCCGCCGCCGTCGCACCACTGCCGAATTCGCGCAA
GGTCTACGTGACGGGCTCGCAACCCGACATCCGCGTGCCGATGCGTGAAATCACGCAAGCCGATACGCCGACGAGCTTCG
GCGGCGAGAAGAATCCGCCGATCTACGTCTACGACACATCGGGCCCGTACACGGACCCGGACGCGAAGATCGACATCCGC
GCGGGCCTGCCCGCGCTGCGCCAGCGCTGGATCGACGCGCGCGGCGACACCGAGACGCTCGCGGGCCTCACGAGCGAATA
CGGCCGCGAGCGCGCGGCCGACCCGGCGACCGCCGAGCTGCGCTTCCCCGATCTGCACCGTCATCCGCGCCGCGCCAAAG
CCGGCAGGAACGTCACGCAGATGCACTACGCGCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCGATCCGCGAG
AACCAGCGCCGCGCCGAGTATCTGGAAAGCCTGAAGGCGAGCGGCCCGAACGGCGCGAAGCTCGCCGCGATGATGGGCCG
CCAGCACGCGGGCCAGGCGTTCGGCGCCGCCGCGTTCGGCGCAAACGAGGCCGGCACGAATATGCTGACCGAGATCACGC
CGGAATTCGTGCGCTCGGAAGTGGCGTGCGGCCGCGCGATCATTCCGGCGAACATCAACCACCCGGAAACCGAGCCGATG
ATCATCGGCCGCAACTTCCTCGTGAAGATCAACGCGAACATCGGCAACTCGGCCGTCACGTCGTCGATCGGCGAGGAAGT
CGACAAGATGACGTGGGCGATCCGCTGGGGCGGCGACACGGTGATGGACTTGTCGACCGGCAAGCACATCCATGAAACGC
GCGAGTGGATCATCCGCAACAGCCCGGTGCCGATCGGCACGGTGCCGATCTACCAGGCGCTGGAAAAGGTCAACGGCAAG
GCCGAGGACCTGACCTGGGAAATCTTCCGCGACACGCTGATCGAGCAGGCCGAGCAAGGCGTCGACTACTTCACGATCCA
CGCGGGCGTGCGCCTGCAGTACGTGCCGCTCACCGCGAACCGGATGACGGGCATCGTGTCGCGCGGCGGCTCGATCATGG
CGAAGTGGTGTCTCGCGCATCACAAGGAGAGCTTCCTGTACGAGCACTTCGAAGAGATCTGCGAAATCATGAAGGCGTAC
GACGTGAGCTTCTCGCTCGGCGACGGCCTGCGCCCCGGCTCGATCTACGACGCGAACGACGAAGCGCAGTTGGGCGAACT
GAAGACGCTCGGCGAGCTCACGCAAATCGCGTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGA
TGCAGTTGATCAAGGAGAACATGGATCTGCAGCTCGACTGGTGCAAGGAAGCGCCGTTCTACACGCTCGGGCCGCTCACG
ACCGACATCGCGCCCGGCTACGACCACATCACGTCGGGCATCGGCGCCGCGATGATCGGCTGGTTCGGCACCGCGATGCT
CTGCTACGTGACACCGAAGGAACACCTCGGCCTGCCGAACAAGGACGACGTGAAGGAAGGCATCATCACGTACAAGCTCG
CCGCGCATGCCGCGGACCTCGCGAAGGGCCACCCGGGCGCGCAGGTGCGCGACAACGCGTTGTCGAAGGCGCGCTTCGAG
TTCCGCTGGGAAGACCAGTTCAACCTCGGCCTCGATCCGGACAAGGCGCGCGAATTCCACGACGAAACGCTGCCGAAGGA
TTCGGCGAAGGTCGCGCACTTCTGCTCGATGTGCGGCCCGCACTTCTGCTCGATGAAGATCACGCAGGACGTGCGCGAGT
TCGCCGCGCAGCAAGGCATGTCGGAAGACGATGCGCTGAAGAAGGGGATGGAAGTGAAGGCGGTCGAGTTCGTGAAGACC
GGCTCGGAGATCTATCACCGCCAGTAA

Upstream 100 bases:

>100_bases
CGCGTCGCGAATCTCGTCTCCTGCTTAGCGCCCCACACCCGCTTTGCATGGGACCCGGGCGAGCGCCAAAGCGCCAACTC
GGGTCGACACAGGAGATCGC

Downstream 100 bases:

>100_bases
CGCGGCACACGCCTCGCAGCGCCCCGAAAGCCCGCCTCGCGCGGGCTTTTTTGCGCCCGCGCAACGGCGCTTCGGGGGAC
GAAACAATTGATTACGAAAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MNANPKFLSADARVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTSFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIDARGDTETLAGLTSEYGRERAADPATAELRFPDLHRHPRRAKAGRNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHAGQAFGAAAFGANEAGTNMLTEITPEFVRSEVACGRAIIPANINHPETEPM
IIGRNFLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGK
AEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAY
DVSFSLGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLT
TDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFE
FRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGMSEDDALKKGMEVKAVEFVKT
GSEIYHRQ

Sequences:

>Translated_648_residues
MNANPKFLSADARVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTSFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIDARGDTETLAGLTSEYGRERAADPATAELRFPDLHRHPRRAKAGRNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHAGQAFGAAAFGANEAGTNMLTEITPEFVRSEVACGRAIIPANINHPETEPM
IIGRNFLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGK
AEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAY
DVSFSLGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLT
TDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFE
FRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGMSEDDALKKGMEVKAVEFVKT
GSEIYHRQ
>Mature_648_residues
MNANPKFLSADARVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTSFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIDARGDTETLAGLTSEYGRERAADPATAELRFPDLHRHPRRAKAGRNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHAGQAFGAAAFGANEAGTNMLTEITPEFVRSEVACGRAIIPANINHPETEPM
IIGRNFLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGK
AEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAY
DVSFSLGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLT
TDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFE
FRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGMSEDDALKKGMEVKAVEFVKT
GSEIYHRQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=642, Percent_Identity=68.6915887850467, Blast_Score=903, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_BURTA (Q2SUP3)

Other databases:

- EMBL:   CP000086
- RefSeq:   YP_443356.1
- STRING:   Q2SUP3
- GeneID:   3848206
- GenomeReviews:   CP000086_GR
- KEGG:   bte:BTH_I2844
- TIGR:   BTH_I2844
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   BTHA271848:BTH_I2844-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 71754; Mature: 71754

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: BINDING 253-253 BINDING 282-282 BINDING 311-311 BINDING 347-347 BINDING 447-447 BINDING 474-474

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANPKFLSADARVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTSFGGEKNPP
CCCCCCEEECCCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPDAKIDIRAGLPALRQRWIDARGDTETLAGLTSEYGRERAADPATAEL
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
RFPDLHRHPRRAKAGRNVTQMHYARQGIITPEMEFIAIRENQRRAEYLESLKASGPNGAK
ECCHHHCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHAGQAFGAAAFGANEAGTNMLTEITPEFVRSEVACGRAIIPANINHPETEPM
HHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCCE
IIGRNFLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRN
EECCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEECCCEEEECCCCCHHHHHHHHHHCC
SPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTAN
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHH
RMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFSLGDGLRPGSIYDAND
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCC
EAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLT
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCC
TDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADL
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHEEEHHHHHHHHH
AKGHPGAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGP
HCCCCCCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
HFCSMKITQDVREFAAQQGMSEDDALKKGMEVKAVEFVKTGSEIYHRQ
HHEEEHHHHHHHHHHHHCCCCCCHHHHCCCCEEEHHHHHCCHHHHCCC
>Mature Secondary Structure
MNANPKFLSADARVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTSFGGEKNPP
CCCCCCEEECCCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPDAKIDIRAGLPALRQRWIDARGDTETLAGLTSEYGRERAADPATAEL
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
RFPDLHRHPRRAKAGRNVTQMHYARQGIITPEMEFIAIRENQRRAEYLESLKASGPNGAK
ECCHHHCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHAGQAFGAAAFGANEAGTNMLTEITPEFVRSEVACGRAIIPANINHPETEPM
HHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCCE
IIGRNFLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRN
EECCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEECCCEEEECCCCCHHHHHHHHHHCC
SPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTAN
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHH
RMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFSLGDGLRPGSIYDAND
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCC
EAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLT
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCC
TDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADL
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHEEEHHHHHHHHH
AKGHPGAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGP
HCCCCCCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
HFCSMKITQDVREFAAQQGMSEDDALKKGMEVKAVEFVKTGSEIYHRQ
HHEEEHHHHHHHHHHHHCCCCCCHHHHCCCCEEEHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA