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The map label for this gene is argF-2 [H]

Identifier: 83719497

GI number: 83719497

Start: 2713179

End: 2714189

Strand: Direct

Name: argF-2 [H]

Synonym: BTH_I2385

Alternate gene names: 83719497

Gene position: 2713179-2714189 (Clockwise)

Preceding gene: 83718749

Following gene: 83720629

Centisome position: 71.23

GC content: 64.49

Gene sequence:

>1011_bases
ATGAGCTTCAACCTGCACAATCGCAACCTGCTGAGCCTGATCCACCACAGCGCGCGCGAGCTGCGCTATCTGCTCGACCT
CGCGCGGGATCTCAAGCGCGCGAAGTACAGCGGCACCGAGCAGCCGCGCCTCTTGCGCAAGAACATCGCGCTCATCTTCG
AAAAGACGTCCACCCGCACGCGCTGCGCGTTCGAAGTCGCCGCGTACGACCAGGGCGCGAACGTCACGTACATCGATCCG
GCTTCGTCGCAGATCGGCCACAAGGAAAGCATGAAGGACACGGCGCGCGTGCTCGGCCGGATGTACGACGCGATCGAGTA
CCGGGGCTTCAGCCAGGAGATCGTCGAGGAGCTCGCGCACCATGCGGGCGTGCCCGTCTTCAACGGGCTTACCGACGAAT
ACCACCCGACGCAGATGCTCGCCGACGTGATGACGATGCGCGAGCACAGCGACAAGCCGCTGCACGACATCAGCTACGCG
TATCTCGGCGACGCGCGCAACAACATGGGCAACTCGCTGCTTCTGATCGGCGCGAAGCTTGGGATGGACGTGCGCATCGG
CGCGCCGAAGTCGCTCTGGCCCGCCGCCGATTTCATCGCGCAGTGCGAGGCGTTCGCGGCCGAGAGCGGCGCGCGGCTCA
CGCTGACCGAGGACCCGTACGAAGCGGTCAAGGGCGTCGACTTCATTCACACCGACGTGTGGGTATCGATGGGCGAGCCC
GTCGAGGCGTGGGACGAGCGGATCAACGCGCTGCTGCCGTATCAGGTGAACCGCAAGCTGATCGAATCGACGGGCAATCC
GCGCACGCGCTTCATGCACTGCCTGCCGTCGTTCCACAACTGCGACACGAAGGTCGGCAAGCAGATCGCCGAGCGCTATC
CGCACCTGAAGGACGGCATCGAAGTGACCGACGAAGTGTTCGAGTCGCCGCGCTGCATCGCGTTCGAGCAGGCGGAAAAC
CGCATGCACACGATCAAGGCGGTCCTCGTGTCGACTCTGGGCGGCATCTGA

Upstream 100 bases:

>100_bases
TGCATGACCTGCCCGGTGCTGCGCGATCCCGTCGACTACTGATGCCAAGCGTCGCGACGGCGCTCGCGCCGCCGCCCCCG
ACAACCCACGGAGAAACCAG

Downstream 100 bases:

>100_bases
CGCTCGGCGCGCACCATCGAACCGGACGGCGCGACGCCTCGCCGTCCGCACCGTCCGCACTACAAGGAGAAACACGATGC
GTATCGTCATCGCATTGGGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSFNLHNRNLLSLIHHSARELRYLLDLARDLKRAKYSGTEQPRLLRKNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDP
ASSQIGHKESMKDTARVLGRMYDAIEYRGFSQEIVEELAHHAGVPVFNGLTDEYHPTQMLADVMTMREHSDKPLHDISYA
YLGDARNNMGNSLLLIGAKLGMDVRIGAPKSLWPAADFIAQCEAFAAESGARLTLTEDPYEAVKGVDFIHTDVWVSMGEP
VEAWDERINALLPYQVNRKLIESTGNPRTRFMHCLPSFHNCDTKVGKQIAERYPHLKDGIEVTDEVFESPRCIAFEQAEN
RMHTIKAVLVSTLGGI

Sequences:

>Translated_336_residues
MSFNLHNRNLLSLIHHSARELRYLLDLARDLKRAKYSGTEQPRLLRKNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDP
ASSQIGHKESMKDTARVLGRMYDAIEYRGFSQEIVEELAHHAGVPVFNGLTDEYHPTQMLADVMTMREHSDKPLHDISYA
YLGDARNNMGNSLLLIGAKLGMDVRIGAPKSLWPAADFIAQCEAFAAESGARLTLTEDPYEAVKGVDFIHTDVWVSMGEP
VEAWDERINALLPYQVNRKLIESTGNPRTRFMHCLPSFHNCDTKVGKQIAERYPHLKDGIEVTDEVFESPRCIAFEQAEN
RMHTIKAVLVSTLGGI
>Mature_335_residues
SFNLHNRNLLSLIHHSARELRYLLDLARDLKRAKYSGTEQPRLLRKNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDPA
SSQIGHKESMKDTARVLGRMYDAIEYRGFSQEIVEELAHHAGVPVFNGLTDEYHPTQMLADVMTMREHSDKPLHDISYAY
LGDARNNMGNSLLLIGAKLGMDVRIGAPKSLWPAADFIAQCEAFAAESGARLTLTEDPYEAVKGVDFIHTDVWVSMGEPV
EAWDERINALLPYQVNRKLIESTGNPRTRFMHCLPSFHNCDTKVGKQIAERYPHLKDGIEVTDEVFESPRCIAFEQAENR
MHTIKAVLVSTLGGI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=330, Percent_Identity=39.0909090909091, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1790703, Length=331, Percent_Identity=58.9123867069486, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=58.1325301204819, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI48994908, Length=381, Percent_Identity=24.1469816272966, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI2367364, Length=172, Percent_Identity=34.8837209302326, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=330, Percent_Identity=31.5151515151515, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322331, Length=163, Percent_Identity=27.6073619631902, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24642586, Length=341, Percent_Identity=24.0469208211144, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 37880; Mature: 37748

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNLHNRNLLSLIHHSARELRYLLDLARDLKRAKYSGTEQPRLLRKNIALIFEKTSTRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEECCCCCC
RCAFEVAAYDQGANVTYIDPASSQIGHKESMKDTARVLGRMYDAIEYRGFSQEIVEELAH
CEEEEEEEECCCCCEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
HAGVPVFNGLTDEYHPTQMLADVMTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCEEEEEEECC
GMDVRIGAPKSLWPAADFIAQCEAFAAESGARLTLTEDPYEAVKGVDFIHTDVWVSMGEP
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHHCCCCC
VEAWDERINALLPYQVNRKLIESTGNPRTRFMHCLPSFHNCDTKVGKQIAERYPHLKDGI
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCH
EVTDEVFESPRCIAFEQAENRMHTIKAVLVSTLGGI
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFNLHNRNLLSLIHHSARELRYLLDLARDLKRAKYSGTEQPRLLRKNIALIFEKTSTRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEECCCCCC
RCAFEVAAYDQGANVTYIDPASSQIGHKESMKDTARVLGRMYDAIEYRGFSQEIVEELAH
CEEEEEEEECCCCCEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
HAGVPVFNGLTDEYHPTQMLADVMTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCEEEEEEECC
GMDVRIGAPKSLWPAADFIAQCEAFAAESGARLTLTEDPYEAVKGVDFIHTDVWVSMGEP
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHHCCCCC
VEAWDERINALLPYQVNRKLIESTGNPRTRFMHCLPSFHNCDTKVGKQIAERYPHLKDGI
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCH
EVTDEVFESPRCIAFEQAENRMHTIKAVLVSTLGGI
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA