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The map label for this gene is pucI [H]

Identifier: 83719446

GI number: 83719446

Start: 2338228

End: 2339718

Strand: Direct

Name: pucI [H]

Synonym: BTH_I2065

Alternate gene names: 83719446

Gene position: 2338228-2339718 (Clockwise)

Preceding gene: 83720494

Following gene: 83721450

Centisome position: 61.38

GC content: 64.86

Gene sequence:

>1491_bases
ATGGCTCAGTTCAGTGTGGCGCAGCAAAGCGCCTCGTATCGGCCGAACGAGGGGCGCCCGGGCGACCCGGACGGCGGCGC
GGCGATGCCCGCCGGCTACAGCAAACGTCTGTACAACGAAGATCTCGCGCCGCTCGCGAACCAGAACTGGGGGGCATATA
ACATCTTCGCGTTCTGGATGTCGGACGTGCACAGCGTCGGCGGCTACGTGTTCGCGGGCAGCCTGTTCGCGCTCGGCCTG
ACGAGCTGGCAGGTGCTCGCCGCGCTGATCGTCGGCATTTCGATCGTCAACGTGCTGTGCAACCTGATCGCGAAGCCGAG
CCAGCAGCTCGGCGTGCCGTATCCGGTGGCATGCCGCGCGACGTTCGGCGTGCTCGGGGCGAACGTGCCCGCGGTGATTC
GCGGCCTCATCGCGATCGCATGGTACGGAATCCAAACTTACCTCGCGTCGAGCGCGCTCGTGATCGTCGTGCTCAAGTTC
TTTCCGCAATGGATGCCGTACGCGGACGTGCATCGCTATGGCTTTCTCGGGCTGTCGGCGCTCGGCTGGGCGGGCTTCAT
GCTGCTGTGGGTGCTGCAGGCGTTCGTGTTCTGGAACGGCATGGAGACGATCAAGAAGTTCATCGATTTCGCCGGTCCTG
CGGTGTACGTGGTGATGTTCGTCCTCGCGGGCTACATGGTGTGGCGCGCGGGCTGGCGCAACATTGGCCTCGATCTCGGC
GGCGTCAAGTATCACGGCGCCGAAGTGCTTCCGGTGATGGTGACGGCGATCTCGCTCGTCGTGTCGTATTTCTCCGGACC
GATGCTCAACTTCGGCGACTTCTCGCGGTACTGCAGGAGCTACGGCAGCGTGAAGCGCGGCAATTTCTGGGGATTGCCCG
TCAACTTTCTCGCGTTCTCGCTCGTCACCGTCATCACGACGGCCGCGACGCTGCCGGTGTTCGGACAACTGATCACGGAC
CCCGTCGAGACGGTCGGCCGCATCGATCATCCGACCGCCGTGATTCTCGGCGCGCTGACCTTCACGATCGCGACGATCGG
CATCAACATCGTCGCGAACTTCGTGTCGCCCGCATTCGACTTCTCGAACGTCGCGCCGCGCCTGATCAGCTGGCGTGCGG
GCGGGATGCTCGCGGCGGTTGCCTCGGTGTTCATCACGCCGTGGAATCTCTTCAACAATCCCGCGGTGATCCATTACACG
CTCGACGTGCTCGGCAGCTTCATCGGGCCGCTGTATGGCGTGCTGATCGTCGATTTCTACCTCGTGAAGCGCGGCGCGCT
GCGGCGCGACGATCTGTATACGACGTCGGCCGACGGCGCGTACTGGTATCGCGACGGCGTGAACCGGCGCGCGGTCGCCG
CGTTGTTGCCCGCGGCCGCGATCGCGGTTGCGTGCGTGATGGTGCCCGCGCCGTCCGGGCTCGCGAACTTCTCCTGGTTC
ATCGGCGCGGCGCTCGGCGGTGCGTTCTACCGCGCGCTTGCGAAAGCATGA

Upstream 100 bases:

>100_bases
CGCGTGCGGACGCCTCGCCAGGGCGGCATCGGGCGATCACGCTCGAGCCGCGATTTCAATGACGATCGCGCGGCTCGTCC
GCGGCAGGCAAGGAGATGTC

Downstream 100 bases:

>100_bases
TGGAGTCCGTATGAAAATAAAACTGATCAATCCGAACACGACGCAGCGGATGACCGACGCGATGGGCCGCTGCGCGCGCG
AGGTCGCGGCGGCCGGCACG

Product: NCS1 nucleoside transporter family protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAQFSVAQQSASYRPNEGRPGDPDGGAAMPAGYSKRLYNEDLAPLANQNWGAYNIFAFWMSDVHSVGGYVFAGSLFALGL
TSWQVLAALIVGISIVNVLCNLIAKPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKF
FPQWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFVLAGYMVWRAGWRNIGLDLG
GVKYHGAEVLPVMVTAISLVVSYFSGPMLNFGDFSRYCRSYGSVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGQLITD
PVETVGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYT
LDVLGSFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAVAALLPAAAIAVACVMVPAPSGLANFSWF
IGAALGGAFYRALAKA

Sequences:

>Translated_496_residues
MAQFSVAQQSASYRPNEGRPGDPDGGAAMPAGYSKRLYNEDLAPLANQNWGAYNIFAFWMSDVHSVGGYVFAGSLFALGL
TSWQVLAALIVGISIVNVLCNLIAKPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKF
FPQWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFVLAGYMVWRAGWRNIGLDLG
GVKYHGAEVLPVMVTAISLVVSYFSGPMLNFGDFSRYCRSYGSVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGQLITD
PVETVGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYT
LDVLGSFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAVAALLPAAAIAVACVMVPAPSGLANFSWF
IGAALGGAFYRALAKA
>Mature_495_residues
AQFSVAQQSASYRPNEGRPGDPDGGAAMPAGYSKRLYNEDLAPLANQNWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLT
SWQVLAALIVGISIVNVLCNLIAKPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFF
PQWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFVLAGYMVWRAGWRNIGLDLGG
VKYHGAEVLPVMVTAISLVVSYFSGPMLNFGDFSRYCRSYGSVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGQLITDP
VETVGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYTL
DVLGSFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAVAALLPAAAIAVACVMVPAPSGLANFSWFI
GAALGGAFYRALAKA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=471, Percent_Identity=28.4501061571125, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6319495, Length=438, Percent_Identity=25.1141552511416, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322219, Length=438, Percent_Identity=23.0593607305936, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319429, Length=514, Percent_Identity=22.9571984435798, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6323266, Length=472, Percent_Identity=22.0338983050847, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324766, Length=472, Percent_Identity=21.8220338983051, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324645, Length=468, Percent_Identity=20.0854700854701, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53500; Mature: 53369

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQFSVAQQSASYRPNEGRPGDPDGGAAMPAGYSKRLYNEDLAPLANQNWGAYNIFAFWM
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHH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HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]