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The map label for this gene is purR [H]

Identifier: 83719248

GI number: 83719248

Start: 924817

End: 925848

Strand: Direct

Name: purR [H]

Synonym: BTH_I0812

Alternate gene names: 83719248

Gene position: 924817-925848 (Clockwise)

Preceding gene: 83719792

Following gene: 83720632

Centisome position: 24.28

GC content: 62.11

Gene sequence:

>1032_bases
ATGGGACGCAAGAGTCATTCGCACACGGTGACGCTGAAGGACGTGGCGCGCGAAGCCGAGGTGTCATACCAGACCGTGTC
GCGTGCCATCAACGGATACGATGAAATCAGCGCGGAAACGCGCGAGAAGGTGCTCGATGTCTGCCGTCGGCTAGGCTATC
GCCCCAACCGCCTGGCGGGCAGTTTGCGATCGAAACGCTCGAATGTCGTGGGCCTCATTGTGTCCGATATCGAAAACGTG
TTTTTTGCCGAGGTGGCGAGTGGCGTCGAATCCGAGGCGAGACACAAGGGTTATTCGGTGTTGCTCGCGAATACGGCCGA
AGACATTGTGCGCGAACGCGAGGCGGTAGGGCAATTCTTCGAGCGGCGCGTCGACGGGCTGATTCTTGCGCCGAGCGAGG
GAGAGCACGATTACCTGCGCACCGAGCTGCCGAAGACGTTTCCAATTGTGGCGGTCAATCGCGAGCTGCGGATTCCGGGC
TGCGGCGCGGTGCTGTCCGAAAATGTGCGAGGCGCGCGCACCGCGGTCGAGTATCTGATCGCACGCGGCCATACGCGCAT
CGGCGCGATCGTTGGCAGCGCGGGGCTGATGACGTCGCGTGAACGGTTGAAGGGCTTTCGCGCCGCAATGTCCGCGGCGG
GCCTGCCGGTCCGCCAGGAGTGGATCGCCGCAGGTGGCGTACGCGCGGACAACGGCCGCGACGGGGCGATCAAAGTGCTC
ACGGGTGCGGATCGCCCCACGGCATTGCTGACGTCGAGCCATCGGATCACCGAAGGGGCCATGCAGGCGCTGAACGTTCT
AGGCTTGCGCTATGGGCCGGACGTGGAAATCGTAAGCTTCGACAATTTACCGTGGATGGCCTTTCTCGATCCGCCACTAC
CCGTGGTCGAGCAACCGACCCGGCGCATCGGGCAGGAAGCCATGCGCATGCTGATACACATGATCGAAGGCACGGGCAAT
GCGACGGAAATGCGTTTACAGACTCGCTTTGTCACGCACGTGGGTCAGGATTTAAGCGTCGAAGCAGAATGA

Upstream 100 bases:

>100_bases
GCGGTGATCCTGGAGCGGGTGCGCACTGGCCGTCGGACCGGCGCAGGTTGGCGGCGAGATCGGAGTCGGGTATCGTTCTG
CGGATAACCAGGAAAGAGTC

Downstream 100 bases:

>100_bases
CTCGATGTCCACCCACGTCAGGTCATCGGGCTCGGAAGTAGGTGTTCTGTTTGCCCGCGTATATTTGTGTCGCGCTGATC
GGAGTTAATGCGGACTTCCC

Product: ribose operon repressor

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MGRKSHSHTVTLKDVAREAEVSYQTVSRAINGYDEISAETREKVLDVCRRLGYRPNRLAGSLRSKRSNVVGLIVSDIENV
FFAEVASGVESEARHKGYSVLLANTAEDIVREREAVGQFFERRVDGLILAPSEGEHDYLRTELPKTFPIVAVNRELRIPG
CGAVLSENVRGARTAVEYLIARGHTRIGAIVGSAGLMTSRERLKGFRAAMSAAGLPVRQEWIAAGGVRADNGRDGAIKVL
TGADRPTALLTSSHRITEGAMQALNVLGLRYGPDVEIVSFDNLPWMAFLDPPLPVVEQPTRRIGQEAMRMLIHMIEGTGN
ATEMRLQTRFVTHVGQDLSVEAE

Sequences:

>Translated_343_residues
MGRKSHSHTVTLKDVAREAEVSYQTVSRAINGYDEISAETREKVLDVCRRLGYRPNRLAGSLRSKRSNVVGLIVSDIENV
FFAEVASGVESEARHKGYSVLLANTAEDIVREREAVGQFFERRVDGLILAPSEGEHDYLRTELPKTFPIVAVNRELRIPG
CGAVLSENVRGARTAVEYLIARGHTRIGAIVGSAGLMTSRERLKGFRAAMSAAGLPVRQEWIAAGGVRADNGRDGAIKVL
TGADRPTALLTSSHRITEGAMQALNVLGLRYGPDVEIVSFDNLPWMAFLDPPLPVVEQPTRRIGQEAMRMLIHMIEGTGN
ATEMRLQTRFVTHVGQDLSVEAE
>Mature_342_residues
GRKSHSHTVTLKDVAREAEVSYQTVSRAINGYDEISAETREKVLDVCRRLGYRPNRLAGSLRSKRSNVVGLIVSDIENVF
FAEVASGVESEARHKGYSVLLANTAEDIVREREAVGQFFERRVDGLILAPSEGEHDYLRTELPKTFPIVAVNRELRIPGC
GAVLSENVRGARTAVEYLIARGHTRIGAIVGSAGLMTSRERLKGFRAAMSAAGLPVRQEWIAAGGVRADNGRDGAIKVLT
GADRPTALLTSSHRITEGAMQALNVLGLRYGPDVEIVSFDNLPWMAFLDPPLPVVEQPTRRIGQEAMRMLIHMIEGTGNA
TEMRLQTRFVTHVGQDLSVEAE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=32.8125, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1790194, Length=308, Percent_Identity=33.7662337662338, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1787948, Length=307, Percent_Identity=32.5732899022801, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=29.4117647058824, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=30.3225806451613, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=30.2547770700637, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=29.3515358361775, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1787580, Length=329, Percent_Identity=25.531914893617, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787906, Length=309, Percent_Identity=28.4789644012945, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786268, Length=307, Percent_Identity=27.3615635179153, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790715, Length=333, Percent_Identity=25.2252252252252, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=27.0769230769231, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790689, Length=310, Percent_Identity=24.5161290322581, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37597; Mature: 37466

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKSHSHTVTLKDVAREAEVSYQTVSRAINGYDEISAETREKVLDVCRRLGYRPNRLAG
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SLRSKRSNVVGLIVSDIENVFFAEVASGVESEARHKGYSVLLANTAEDIVREREAVGQFF
HHHHHCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
ERRVDGLILAPSEGEHDYLRTELPKTFPIVAVNRELRIPGCGAVLSENVRGARTAVEYLI
HHHHCCEEECCCCCCCHHHHHCCCCCCCEEEECCEECCCCCHHHHHCCCCHHHHHHHHHH
ARGHTRIGAIVGSAGLMTSRERLKGFRAAMSAAGLPVRQEWIAAGGVRADNGRDGAIKVL
HCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEECCCCCCCEEEEE
TGADRPTALLTSSHRITEGAMQALNVLGLRYGPDVEIVSFDNLPWMAFLDPPLPVVEQPT
ECCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHH
RRIGQEAMRMLIHMIEGTGNATEMRLQTRFVTHVGQDLSVEAE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure 
GRKSHSHTVTLKDVAREAEVSYQTVSRAINGYDEISAETREKVLDVCRRLGYRPNRLAG
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SLRSKRSNVVGLIVSDIENVFFAEVASGVESEARHKGYSVLLANTAEDIVREREAVGQFF
HHHHHCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
ERRVDGLILAPSEGEHDYLRTELPKTFPIVAVNRELRIPGCGAVLSENVRGARTAVEYLI
HHHHCCEEECCCCCCCHHHHHCCCCCCCEEEECCEECCCCCHHHHHCCCCHHHHHHHHHH
ARGHTRIGAIVGSAGLMTSRERLKGFRAAMSAAGLPVRQEWIAAGGVRADNGRDGAIKVL
HCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEECCCCCCCEEEEE
TGADRPTALLTSSHRITEGAMQALNVLGLRYGPDVEIVSFDNLPWMAFLDPPLPVVEQPT
ECCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHH
RRIGQEAMRMLIHMIEGTGNATEMRLQTRFVTHVGQDLSVEAE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA