Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 83719122

GI number: 83719122

Start: 3429410

End: 3430051

Strand: Reverse

Name: hisH [H]

Synonym: BTH_I2989

Alternate gene names: 83719122

Gene position: 3430051-3429410 (Counterclockwise)

Preceding gene: 83721255

Following gene: 83719864

Centisome position: 90.05

GC content: 67.29

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCGATTGTGGATTATGGAATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCATGAAGGCGGAACC
CGCGGCCGACGTGAAGATCGTCGACCGGCCCGAGGCGATCCGCGCGGCGGACCGCGTGGTGCTGCCCGGCCAGGGCGCGA
TGCCCGACTGCATGCGCTGCCTGGGCGAGTCCGGCCTGCAGGAGGCGGTGGTCGAGGCGTCGCGCACGAAGCCGCTCCTC
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGGCACGAAAGGGCTCGGCCTGCTGCCGGGCAA
GGTCGTGCGCTTCGCGCTCGAAGGCCGGCTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAAATGGGCTGGAACCGCG
TGCACCAGACGCAGGCGCATCCGCTGTGGGACGGCGTGCCGGACGACGCGTACTTCTATTTCGTGCACAGCTATTACGTG
GTGCCGGACAACCCGGCGCACACCGCGGGCGAGACCCGGTACGGCGACGTGTTCACGTCCGCGGTCGCGCGCGACAATCT
CTTCGCGACCCAATTTCACCCGGAAAAGAGCGCCGAAGTGGGGCTGCGGCTTTACCGGAATTTTGTCCACTGGAACCCCT
GA

Upstream 100 bases:

>100_bases
CCGGCATCAACATCGTCACGCGTCTGATGGGATTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCGACGGACTGAAG
GCATTGTTGCCTGCACTGAC

Downstream 100 bases:

>100_bases
ACGTGGCGCGAGAGCCACACCCGGCGCGCGCAAAAGGCCCGCCGCACGGGCTTCGCGAGCGGATACGCAGAAAGAGTTGT
ACTAAACTAGCGGGACGGCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGGTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVHQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETRYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGGTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVHQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETRYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGGTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVHQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETRYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=36.5384615384615, Blast_Score=125, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=31.651376146789, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23479; Mature: 23479

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGGTKGLGLLPGKVVRFALEGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
GSRFKVPQMGWNRVHQTQAHPLWDGVPDDAYFYFVHSYYVVPDNPAHTAGETRYGDVFTS
CCEEECCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHHHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGGTKGLGLLPGKVVRFALEGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
GSRFKVPQMGWNRVHQTQAHPLWDGVPDDAYFYFVHSYYVVPDNPAHTAGETRYGDVFTS
CCEEECCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHHHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]