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The map label for this gene is tilS/hprT [H]

Identifier: 83719079

GI number: 83719079

Start: 1293999

End: 1294598

Strand: Reverse

Name: tilS/hprT [H]

Synonym: BTH_I1148

Alternate gene names: 83719079

Gene position: 1294598-1293999 (Counterclockwise)

Preceding gene: 83719534

Following gene: 83719033

Centisome position: 33.99

GC content: 63.5

Gene sequence:

>600_bases
TTGTTTCATTCTTATTGGCATCCCAGACGAGACCGCTCGCCAAACCTCATGAATCGAGAAGAAGCCCTCCACATTTTCCA
ACACTCCGAAGAGATCGTCTCCGCCGGCGAAGTCAACGGTTCGATCGGCCGGATGGCCGAGGAGATCCGCGGCCAGATCG
GCGAGGAGTTTCCGCTCGTGCTGTCGGTGATGGGCGGTGCGGCGGTGTTCACCGGCATGCTGCTGCCGCACCTCGATTTC
CCGCTCGAGTTCGACTACATCCACCTGACGCGCTACCGCAACACGACGAAGGGCAGCCCCGAAATGCACTGGCGCGTCGC
ACCGCGCGAATCGGTGAAGGACCGCATCGTGCTCGTGCTCGACGACATCCTCGACGAAGGCGAAACGATGGCCGCGATTC
GCGACCGCATCCTCGAGATGGGGGCGAAGCGCTTCCTGTCCGCCGTGCTGTGCGAAAAGACGCTGGCGAAGGCGAAGCCG
CTGCATCCGGATTTCTGCGGGTTCGCGGTGCCCGACCGCTACGTGTTCGGCTGCGGGATGGACGCGAAGGGGTACTGGCG
CAACCTGCCGACGATCCGCGCCCTGACGGCCGACGTCTGA

Upstream 100 bases:

>100_bases
CTGCAGAAGATGATGCGCGGCATAAAGGGCATGATGCCCGGCATGCGCTGATTCGCCGCGGCGCCGCGCACGGGTATATG
CTCTAGCGCGTGGCGTCGCA

Downstream 100 bases:

>100_bases
CGACGCCCCGCGCGGCAGCCCCGGCTACCTCGCGCACGCAACAAGAAAGCGGCTCGCGCATCATGCGCGAGCCGCTTTTC
TTTATCAGGCGCCTCCTGGC

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MFHSYWHPRRDRSPNLMNREEALHIFQHSEEIVSAGEVNGSIGRMAEEIRGQIGEEFPLVLSVMGGAAVFTGMLLPHLDF
PLEFDYIHLTRYRNTTKGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDRILEMGAKRFLSAVLCEKTLAKAKP
LHPDFCGFAVPDRYVFGCGMDAKGYWRNLPTIRALTADV

Sequences:

>Translated_199_residues
MFHSYWHPRRDRSPNLMNREEALHIFQHSEEIVSAGEVNGSIGRMAEEIRGQIGEEFPLVLSVMGGAAVFTGMLLPHLDF
PLEFDYIHLTRYRNTTKGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDRILEMGAKRFLSAVLCEKTLAKAKP
LHPDFCGFAVPDRYVFGCGMDAKGYWRNLPTIRALTADV
>Mature_199_residues
MFHSYWHPRRDRSPNLMNREEALHIFQHSEEIVSAGEVNGSIGRMAEEIRGQIGEEFPLVLSVMGGAAVFTGMLLPHLDF
PLEFDYIHLTRYRNTTKGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDRILEMGAKRFLSAVLCEKTLAKAKP
LHPDFCGFAVPDRYVFGCGMDAKGYWRNLPTIRALTADV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17510817, Length=165, Percent_Identity=26.0606060606061, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 22615; Mature: 22615

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHSYWHPRRDRSPNLMNREEALHIFQHSEEIVSAGEVNGSIGRMAEEIRGQIGEEFPLV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHH
LSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNTTKGSPEMHWRVAPRESVKDRIVLVL
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHH
DDILDEGETMAAIRDRILEMGAKRFLSAVLCEKTLAKAKPLHPDFCGFAVPDRYVFGCGM
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
DAKGYWRNLPTIRALTADV
CCCHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MFHSYWHPRRDRSPNLMNREEALHIFQHSEEIVSAGEVNGSIGRMAEEIRGQIGEEFPLV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHH
LSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNTTKGSPEMHWRVAPRESVKDRIVLVL
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHH
DDILDEGETMAAIRDRILEMGAKRFLSAVLCEKTLAKAKPLHPDFCGFAVPDRYVFGCGM
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
DAKGYWRNLPTIRALTADV
CCCHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]