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The map label for this gene is rhtB [H]

Identifier: 83719075

GI number: 83719075

Start: 546419

End: 547033

Strand: Direct

Name: rhtB [H]

Synonym: BTH_I0494

Alternate gene names: 83719075

Gene position: 546419-547033 (Clockwise)

Preceding gene: 83719836

Following gene: 83721295

Centisome position: 14.34

GC content: 70.73

Gene sequence:

>615_bases
ATGCCGAACTTCATGCTGTTTCTCGCGACGTCGATCGCGATCACCGTCGCGCCCGGCCCCGACAACCTGCAGGTGCTCGC
GCGCGGCATCTCGCAAGGCCGCGCGGCGGGCATCGTCGCGGCGCTCGGCTTCGCGTCGGGCGTGCTGTTCCATACGACGC
TCGCCGCGTTCGGCGTGGCCGCGCTGCTGCGCTCGTCGCCCGTCGCGTTCCATCTGCTCAAGCTCGCGGGCGGCGCGTAT
CTGATCTGGATCGGCATCAAGGCGCTGCGCAGCAAGGGCCTCGCGACCGCGCACGAGCGCGCGCCGCAGCCGCTGTCGAC
GATCTTCCGCCAGAGCGTGATCGGCAATCTGCTCAATCCGAAGGTGACGCTGTTCTTCGTCGTGTTCCTGCCGCAGTTCG
TCGATCCGCACGGCGCGCAGCCCGTCGCGCTGCAGATGCTCGAACTGGGCGCGCTCTTCATGGCGCAGACGGCCCTGGTG
TTCTCGGTGTTCGGCGTCGGCGCGGGCGCGATCGGCGTGTGGCTCAAGCGCCGGCCGCGCGCCGGCGTGTGGCTCGACCG
CCTCGCGGGCGCGACGTTCGTCGCGCTCGGCCTGCGCGTCGCGCTGAAGGACTGA

Upstream 100 bases:

>100_bases
CGCCCCGCACGCCGGACATCCCCGGCGCCGGTTTCATCCCGCGCTTGCCCGAGCGCGGCCCGTTCGTTCACCGATGTCCA
CCGATTCAAGCGAGCTCACG

Downstream 100 bases:

>100_bases
GGAGGGCGCGATGACGTTTGCGTGCATCGCCGCCGCGCGGCGCTTCGAACCGGCCGCGCACGTGCCGTTCTGGATCGCGG
GCCGGCAGGTGGGCTGGCTG

Product: LysE family protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPNFMLFLATSIAITVAPGPDNLQVLARGISQGRAAGIVAALGFASGVLFHTTLAAFGVAALLRSSPVAFHLLKLAGGAY
LIWIGIKALRSKGLATAHERAPQPLSTIFRQSVIGNLLNPKVTLFFVVFLPQFVDPHGAQPVALQMLELGALFMAQTALV
FSVFGVGAGAIGVWLKRRPRAGVWLDRLAGATFVALGLRVALKD

Sequences:

>Translated_204_residues
MPNFMLFLATSIAITVAPGPDNLQVLARGISQGRAAGIVAALGFASGVLFHTTLAAFGVAALLRSSPVAFHLLKLAGGAY
LIWIGIKALRSKGLATAHERAPQPLSTIFRQSVIGNLLNPKVTLFFVVFLPQFVDPHGAQPVALQMLELGALFMAQTALV
FSVFGVGAGAIGVWLKRRPRAGVWLDRLAGATFVALGLRVALKD
>Mature_203_residues
PNFMLFLATSIAITVAPGPDNLQVLARGISQGRAAGIVAALGFASGVLFHTTLAAFGVAALLRSSPVAFHLLKLAGGAYL
IWIGIKALRSKGLATAHERAPQPLSTIFRQSVIGNLLNPKVTLFFVVFLPQFVDPHGAQPVALQMLELGALFMAQTALVF
SVFGVGAGAIGVWLKRRPRAGVWLDRLAGATFVALGLRVALKD

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=192, Percent_Identity=30.2083333333333, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788099, Length=140, Percent_Identity=35, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786522, Length=113, Percent_Identity=32.7433628318584, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21466; Mature: 21335

Theoretical pI: Translated: 12.13; Mature: 12.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNFMLFLATSIAITVAPGPDNLQVLARGISQGRAAGIVAALGFASGVLFHTTLAAFGVA
CCCHHEEEEEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLRSSPVAFHLLKLAGGAYLIWIGIKALRSKGLATAHERAPQPLSTIFRQSVIGNLLNP
HHHHCCCHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCC
KVTLFFVVFLPQFVDPHGAQPVALQMLELGALFMAQTALVFSVFGVGAGAIGVWLKRRPR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
AGVWLDRLAGATFVALGLRVALKD
CCHHHHHHCCHHHHHHHHHHEECC
>Mature Secondary Structure 
PNFMLFLATSIAITVAPGPDNLQVLARGISQGRAAGIVAALGFASGVLFHTTLAAFGVA
CCHHEEEEEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLRSSPVAFHLLKLAGGAYLIWIGIKALRSKGLATAHERAPQPLSTIFRQSVIGNLLNP
HHHHCCCHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCC
KVTLFFVVFLPQFVDPHGAQPVALQMLELGALFMAQTALVFSVFGVGAGAIGVWLKRRPR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
AGVWLDRLAGATFVALGLRVALKD
CCHHHHHHCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]