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The map label for this gene is pcnB [H]

Identifier: 83719048

GI number: 83719048

Start: 1474332

End: 1475876

Strand: Reverse

Name: pcnB [H]

Synonym: BTH_I1314

Alternate gene names: 83719048

Gene position: 1475876-1474332 (Counterclockwise)

Preceding gene: 83721384

Following gene: 83719979

Centisome position: 38.75

GC content: 69.71

Gene sequence:

>1545_bases
GTGATCAAAAAACTCATCCGCAAGCTGCTCGGGCAGGACGCCCGTACGGCGCAAGACGCGCCGCCGGCCCCGGCCGGCCC
GATCGAGGCCGCGCCCGCCGCGCCGAGCCGCGCGAAATCCGCGCGCGGCGCCGCGAAGAAAACCCGCAACGGCGAGCCGA
CCATCGTGCACGCGAACGCGCACGGGATCGATCCCGCGCTCATCTCGAAAAACGCGATCCGCGTCACCGACACGCTGCAG
CAAGCCGGCTTTCGCGCGTTCATCGTCGGCGGCGCGGTGCGCGACCTGCTGCTCGGCATCGCGCCGAAGGACTTCGACGT
CGCGACCGACGCGACGCCGACCGAGGTCCAGCGCCTGTTTCGCCGCGCGCGGCTGATCGGCCGGCGCTTCCAGATCGTCC
ACGTGCAGTTCGGCCAGGAACTGATCGAAGTGTCGACGTTCCGCGCGCTCGTCGACACGCCGCAGGAGCCGCCCGCCGAC
GCCGCGCCCGCGAAGCGCCTGAAGCGCGACGAACTCGACCGCCGCACGCACGCGGTCGACGCGAGCGGCCGCGTGCTGCG
CGACAACGTGTGGGGCGAGCAGCACGAAGACGCGGCGCGCCGCGACTTCACGATCAACGCGATGTACTACGATCCGGCGT
CGCAGACGGTGCTCGACTACCACGACGGGATGGCCGACATGCGCGCGCGCCTGTTGCGGATGATCGGCGATCCGGCGACG
CGCTACCGCGAGGACCCGGTGCGGATGCTGCGCGTCGTGCGCTTCGCGGCGAAGCTCGGCTTCGACATCGAGGAGAAGAC
GCGCGAGCCGATCAAGGAGCTCGCCGATCTGATCAACAACGTGCCGGCCGCGCGCCTGTTCGACGAAATGCTCAAGCTCC
TGCTGTCGGGCCACGCGCTCGCATGCCTGAAGCAGTTGCGCAAGCAGGGGCTGCACCACGGGCTGCTGCCGCTCCTCGAC
GTCGTGCTCGAACAGCCGCAGGGCGAGAAGTTCGTCACGCTCGCGCTGAACAACACCGACGCGCGCGTGCGCGCCGGCAA
GCCGGTGTCGCCCGGCTTCCTGTTCGCGACGCTCCTTTGGCACGACATGCGCCAGCGCTTCGAGCAATACACGGCCGACG
GCGAATTCCCGGTGCCCGCGCTGCATCGCGCGATGGACGACGTGATCGACATGCAGACCGAGAAGCTCGCGATCCACAAG
CGCTATTCGGCCGACATGCGCGAGATCTGGGGATTGCAGCTGCGCCTCGAGAAGCGCAGCGGCCGCAGCGCGCTGCGCCT
CCTGGAACACCAAAGATTCAGAGCGGGGTATGATTTCCTCCTGTTACGCTGCGAATCCGGCGAGCTCGATCCGGGCGTCG
GACAGTGGTGGACGGATTTCATCGACGGCGATGCCGCGACGCGCGAGGCGCTCCTCACGCAGGGCGGCAGTCGCGAGAAG
TCGCCGCGCAAGCGGCGCCGCCGCAGCGGACGCAGCCGCCGGACGGCCGACGGCGCCGACAGCACGGAAGGCGCCGCGCG
CGGCGAGTCCGACGCGAACGACTGA

Upstream 100 bases:

>100_bases
TCGAAAAAGTCACCGACCCGATCGCGACCAATCCCGACGACACGCTGCGTGCATACGCACGCGAGCACGGCTGGCGCATC
CTCGACCTCTTCCAACCGTC

Downstream 100 bases:

>100_bases
CGCTTGCGCCGTCGAACGACGTAGGAAGGAAAGCCATGACGGTCGCTTATCTCGGGCTGGGGGCCAACCTCGGCGATGCG
CGCCAGGCCTTGAAGGACGC

Product: polyA polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MIKKLIRKLLGQDARTAQDAPPAPAGPIEAAPAAPSRAKSARGAAKKTRNGEPTIVHANAHGIDPALISKNAIRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDTPQEPPAD
AAPAKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPAT
RYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHALACLKQLRKQGLHHGLLPLLD
VVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTADGEFPVPALHRAMDDVIDMQTEKLAIHK
RYSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPGVGQWWTDFIDGDAATREALLTQGGSREK
SPRKRRRRSGRSRRTADGADSTEGAARGESDAND

Sequences:

>Translated_514_residues
MIKKLIRKLLGQDARTAQDAPPAPAGPIEAAPAAPSRAKSARGAAKKTRNGEPTIVHANAHGIDPALISKNAIRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDTPQEPPAD
AAPAKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPAT
RYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHALACLKQLRKQGLHHGLLPLLD
VVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTADGEFPVPALHRAMDDVIDMQTEKLAIHK
RYSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPGVGQWWTDFIDGDAATREALLTQGGSREK
SPRKRRRRSGRSRRTADGADSTEGAARGESDAND
>Mature_514_residues
MIKKLIRKLLGQDARTAQDAPPAPAGPIEAAPAAPSRAKSARGAAKKTRNGEPTIVHANAHGIDPALISKNAIRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDTPQEPPAD
AAPAKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPAT
RYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHALACLKQLRKQGLHHGLLPLLD
VVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTADGEFPVPALHRAMDDVIDMQTEKLAIHK
RYSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPGVGQWWTDFIDGDAATREALLTQGGSREK
SPRKRRRRSGRSRRTADGADSTEGAARGESDAND

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=242, Percent_Identity=28.9256198347107, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081691, Length=414, Percent_Identity=44.4444444444444, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789436, Length=255, Percent_Identity=30.1960784313725, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=312, Percent_Identity=26.6025641025641, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57519; Mature: 57519

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLIRKLLGQDARTAQDAPPAPAGPIEAAPAAPSRAKSARGAAKKTRNGEPTIVHANA
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCC
HGIDPALISKNAIRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQELIEVSTFRALVDTPQEPPADAAPAKRLKRDELDRRTHAVD
HHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPAT
CCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCHHH
RYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHAL
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
ACLKQLRKQGLHHGLLPLLDVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLW
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHH
HDMRQRFEQYTADGEFPVPALHRAMDDVIDMQTEKLAIHKRYSADMREIWGLQLRLEKRS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCC
GRSALRLLEHQRFRAGYDFLLLRCESGELDPGVGQWWTDFIDGDAATREALLTQGGSREK
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCC
SPRKRRRRSGRSRRTADGADSTEGAARGESDAND
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKLIRKLLGQDARTAQDAPPAPAGPIEAAPAAPSRAKSARGAAKKTRNGEPTIVHANA
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCC
HGIDPALISKNAIRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQELIEVSTFRALVDTPQEPPADAAPAKRLKRDELDRRTHAVD
HHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPAT
CCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCHHH
RYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHAL
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
ACLKQLRKQGLHHGLLPLLDVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLW
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHH
HDMRQRFEQYTADGEFPVPALHRAMDDVIDMQTEKLAIHKRYSADMREIWGLQLRLEKRS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCC
GRSALRLLEHQRFRAGYDFLLLRCESGELDPGVGQWWTDFIDGDAATREALLTQGGSREK
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCC
SPRKRRRRSGRSRRTADGADSTEGAARGESDAND
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]