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The map label for this gene is oprM [H]

Identifier: 83718989

GI number: 83718989

Start: 784246

End: 785805

Strand: Direct

Name: oprM [H]

Synonym: BTH_I0682

Alternate gene names: 83718989

Gene position: 784246-785805 (Clockwise)

Preceding gene: 83721001

Following gene: 83720840

Centisome position: 20.59

GC content: 70.83

Gene sequence:

>1560_bases
ATGAAGCGAAAACATGCTTTGACTGCACTCGCAGTCGCGCTGCTCGCCGCGGGCTGCACGCTCGCGCCGCGCTACGAGCG
TCCGGCCGCGCCCGTGTCGGGCGCGTTCCCGACCGACGGCGTCTACGCCGCGCAGCCGGGCGCCGCGGCCGGCGCGCGCA
GCGCGAACGGCCAGGCGGCCGTCGACATCGGCTGGCGCGAGTTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATC
GCGCTGAAGAACAACCGCGATCTGCGCGTGTCGGTGCTCAACGTCGAGGCGTCGCGCGCGCAGTATCGGATCACGCGCGC
GGGCCTGTTCCCGACGCTCGACGGCACCGGCACGGGCACGATCCAGCGCACGCCGGCCGGTGTGTCGGTAACCGGCCAGC
CGCTCATCTCGCGGACCTACAATGTCGGCGTGTCCGCGTCGTGGGAGCTCGACCTGTTCGGCCGCGTGCAGAGCCTGAAG
GATCAGGCGCTCGCGCAATACTTCGCGACCGCGCAGGCGCGCAAGGCCGCGGAGATCTCGCTCGTCGCGAGCGTCGCCGA
TCAGTATCTGACGCTGCTGTCGACCGACGATCTGCTGCAGATCACGGAGAACACGCTGAAGACGGCGCGCGCGTCGTACG
ACCTGACGAAGCTGCAGTTCGACAACGGCACCGGCTCGGAGCTCGATCTGCGGCAGGCGCAGACGGTCGTCGAGACCGCG
CTCGCGAACCAGCAGGCGCAGGCGCGCGCCCGCGCGCAGGCGGTCAACGCGCTCGTGCTGCTGATCGGCGAGCCGCTGCC
CGACGATCTGCCGGCCGGCCTGCCGCTCAATGCGCAGAATCTGCTGACCGACATCCCGGCCGGGCTGCCGTCCGATCTGC
TGACGCGCCGCCCCGACATCATGCAGGCCGAGGAGACGCTGCGCGCGGCGAACGCGAACATCGGCGCGGCGCGTGCGGCG
TTCTTCCCGAAAATCTCGCTCACCGGCGCGTTCGGCACCGCGAGCCCGACGCTCGGCGGCCTGTTCAAGGCGGGCACGGC
GGCGTGGTCGTTCGCGCCGAGCATCGCGCTGCCGATCTTCGAGGGCGGACAGAACATCGCCAATCTCGATCTCGCGCACG
TGCAGAAGCGCATCGAGATCGCGAACTACGAGAAGGCGATCCAGAGCGCGTTCCGCGAGGTGTCGGACGGGCTTGCCGCG
CGCGGCACGTACGATCAGCAGATCGCGGCGCTCGAGCGCAACGAGCACGCGCAGCAGCGCCGCTACGATCTGTCGGACCT
GCGCTACAAGAACGGCGTCGACAGTTATCTGTCGGTGCTGACCGCGCAGACGGATCTGTATTCGGCGCAGCAGCAACTGA
TCAGCGCGCGGCTCGCGCGCTGGACGAACCTCGTCGATCTGTATCGCGCGCTGGGCGGCGGCTGGATCGAACGCGCGGGC
GAGACGCCGCGCCCGGCGGATGCGCCTGTCGACTACGGCAAGGCGGCAGCGCCTGCGCCGGCGTCGGCGTCGGCGTCGGC
GGCGCCCGCTGCGGCGGCCAGCGCGCCGGCGGCCGGCTGA

Upstream 100 bases:

>100_bases
GAAGGAAGACGCCGACGAGGCGCTGCGCCTCGCGCACGAGCACATGCACCGTGACGACAAGCCGGAGCACGGCGACGACG
CTGGCAAGAAGGATTAACAG

Downstream 100 bases:

>100_bases
GCGCTCGCCGGCGCCGCGACGGACGAAGGCAGCCGATTCGTTCGCCGCGCGGCGCGCGATCGGTAGCGAATTCGCCGCAT
CGCGATGCATACGCGAAAAC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPTDGVYAAQPGAAAGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYRITRAGLFPTLDGTGTGTIQRTPAGVSVTGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQITENTLKTARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LANQQAQARARAQAVNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYKNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWIERAG
ETPRPADAPVDYGKAAAPAPASASASAAPAAAASAPAAG

Sequences:

>Translated_519_residues
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPTDGVYAAQPGAAAGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYRITRAGLFPTLDGTGTGTIQRTPAGVSVTGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQITENTLKTARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LANQQAQARARAQAVNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYKNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWIERAG
ETPRPADAPVDYGKAAAPAPASASASAAPAAAASAPAAG
>Mature_519_residues
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPTDGVYAAQPGAAAGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYRITRAGLFPTLDGTGTGTIQRTPAGVSVTGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQITENTLKTARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LANQQAQARARAQAVNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYKNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWIERAG
ETPRPADAPVDYGKAAAPAPASASASAAPAAAASAPAAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=469, Percent_Identity=42.0042643923241, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI2367348, Length=425, Percent_Identity=24.7058823529412, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87082199, Length=315, Percent_Identity=24.4444444444444, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54930; Mature: 54930

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPTDGVYAAQPGAAAGARSANGQAA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
VDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYRITRAGLFPTLDGTGTGT
EECCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEECCCCCCCCCCCCCCC
IQRTPAGVSVTGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEIS
EEECCCCCEECCCHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVASVADQYLTLLSTDDLLQITENTLKTARASYDLTKLQFDNGTGSELDLRQAQTVVETA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LANQQAQARARAQAVNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDI
HHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
MQAEETLRAANANIGAARAAFFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIF
HHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCEECEEE
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQR
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHH
RYDLSDLRYKNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWIERAG
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ETPRPADAPVDYGKAAAPAPASASASAAPAAAASAPAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPTDGVYAAQPGAAAGARSANGQAA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
VDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYRITRAGLFPTLDGTGTGT
EECCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEECCCCCCCCCCCCCCC
IQRTPAGVSVTGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEIS
EEECCCCCEECCCHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVASVADQYLTLLSTDDLLQITENTLKTARASYDLTKLQFDNGTGSELDLRQAQTVVETA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LANQQAQARARAQAVNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDI
HHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
MQAEETLRAANANIGAARAAFFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIF
HHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCEECEEE
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQR
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHH
RYDLSDLRYKNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWIERAG
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ETPRPADAPVDYGKAAAPAPASASASAAPAAAASAPAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]