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The map label for this gene is kefA [H]

Identifier: 83718952

GI number: 83718952

Start: 891348

End: 892700

Strand: Direct

Name: kefA [H]

Synonym: BTH_I0774

Alternate gene names: 83718952

Gene position: 891348-892700 (Clockwise)

Preceding gene: 83719688

Following gene: 83720976

Centisome position: 23.4

GC content: 70.44

Gene sequence:

>1353_bases
ATGGTCACGAATCACCTCACGCGACGGCTCGCGGCCGTCGCACACGACTTCGGCCAGCCGGCGATGATCTGGCAGGCGGT
CGCGTTCCTCGGCTCGCTCGCGCTCGCGTGGCTCGTCGCGCGCTATCTGCGCGGCCGGATCGACGCGCGCCGCCGCACGG
CCGGCCGCGCGCCCGGCCCGGGCGCGCTCAGCCTGAACCGCGCGCTGTTCCCGCTCGTCGGCGGCGTGTTCATGTGGGTC
GCGGAGACGGCGTTCGACGAGCTGATGCCGACGTCGCTGCTGCAGCTCGCGCTCGTGCCGCTCTTCGGCATCGGGCTCAT
CTACATCCTGTTCTTCGTCGCGCGCCGCGTGTTCGCGCGCGACGGCCACACGCACGCGTGGCTGTCGATCGTCGAGAAGT
TCGTGTCGATCGTCGTGTGGATCGCGATGGCGCTCACGGTGCTCGGCATCCAGCGCGACGTGCTCGTCTGGCTCGACAGC
GTCCAGTTCCGCGTCGCGAACGCCCGCCTCACGCTGTTGTCGGTCGTGTCGGGCGCGCTGTGGATCTGCGTGACGCTCGT
CGTCGCGATGTGGCTCGGCTCGGTGCTCGAGGAGCGGCTGATGCGCGCGCGCTCGCTCGACGCGAACCTGCGGGTCGTGC
TCTCGCGCGTCGGTCGCGCGCTGCTCATCTTCGCGGCGGTGCTGATCGGGCTGTCGCTCGTCGGCATCGACGTGACCGTG
CTCGGCGTGTTCGGCGGCGCGCTCGGCGTCGGGCTCGGCTTCGGCCTGCAGAAGATCGCGAGCAACTACGTGTCGGGCTT
CATCATCCTGCTCGACCGGTCGCTGCGGCTCGGCGACGCGATCAACGTCGGCGGCCTGCAGGGCGTCGTCACGCAGATCC
GCACGCGCTATACGGTCGTGCGCGGCCTCGACGGCAACGAGACGCTGATTCCGAACGAGAAGCTCATCACCGACGTCGTG
CAGAACCAGTCGTCGTACCTGACGCGCGGCTATGCGAAGGTCGCGGTTCAGGTGGCGTACACATCGGACGTCGAGCAGGC
GATGGCGCTGCTCGCGGACGCGGCGACGGGCGTCGAGCGCGTGCTCGAGGACCCGGCGCCGACGCCTTATCTTGTCGGCT
TCGGCGCGGACGGGATCGACCTCGAGCTCGGCTTCTGGATCGCCGACGCGGCGAAGGGGACGGCGAGCGTGCGCTCGGCG
GTCAATCGGAACATCTGGCGACTTTTTGTCGCACACGGAATCTCGATTCCGTTCCCGCAGCGGGAGGTGCGCGTGATCGG
GCTGCCGGACGCCATCGCGCCCGCGGCCGGCGCGCACGGCCCGGCGGCGGAGCGGCAGCGGCAGGACGCGTAG

Upstream 100 bases:

>100_bases
CACGGGCGACTATCTGTCGCTCTGGCCGCATCGCCACGCGACCGACGGCTTCTTCGCCGCGGTGCTCGAGCGGCGCGCGC
AGTAACGGGCGGGCTCGCGC

Downstream 100 bases:

>100_bases
CGCCGGGCGGGCGCCGCCGCGCGCCCGTTCAACCTGAAAATCCGCGCGCGCGTATTGCGCCGCCGCAAAAAATATTCATT
TTTTACAAAGACTTGGAACG

Product: mechanosensitive ion channel family protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MVTNHLTRRLAAVAHDFGQPAMIWQAVAFLGSLALAWLVARYLRGRIDARRRTAGRAPGPGALSLNRALFPLVGGVFMWV
AETAFDELMPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVEKFVSIVVWIAMALTVLGIQRDVLVWLDS
VQFRVANARLTLLSVVSGALWICVTLVVAMWLGSVLEERLMRARSLDANLRVVLSRVGRALLIFAAVLIGLSLVGIDVTV
LGVFGGALGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKVAVQVAYTSDVEQAMALLADAATGVERVLEDPAPTPYLVGFGADGIDLELGFWIADAAKGTASVRSA
VNRNIWRLFVAHGISIPFPQREVRVIGLPDAIAPAAGAHGPAAERQRQDA

Sequences:

>Translated_450_residues
MVTNHLTRRLAAVAHDFGQPAMIWQAVAFLGSLALAWLVARYLRGRIDARRRTAGRAPGPGALSLNRALFPLVGGVFMWV
AETAFDELMPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVEKFVSIVVWIAMALTVLGIQRDVLVWLDS
VQFRVANARLTLLSVVSGALWICVTLVVAMWLGSVLEERLMRARSLDANLRVVLSRVGRALLIFAAVLIGLSLVGIDVTV
LGVFGGALGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKVAVQVAYTSDVEQAMALLADAATGVERVLEDPAPTPYLVGFGADGIDLELGFWIADAAKGTASVRSA
VNRNIWRLFVAHGISIPFPQREVRVIGLPDAIAPAAGAHGPAAERQRQDA
>Mature_450_residues
MVTNHLTRRLAAVAHDFGQPAMIWQAVAFLGSLALAWLVARYLRGRIDARRRTAGRAPGPGALSLNRALFPLVGGVFMWV
AETAFDELMPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVEKFVSIVVWIAMALTVLGIQRDVLVWLDS
VQFRVANARLTLLSVVSGALWICVTLVVAMWLGSVLEERLMRARSLDANLRVVLSRVGRALLIFAAVLIGLSLVGIDVTV
LGVFGGALGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKVAVQVAYTSDVEQAMALLADAATGVERVLEDPAPTPYLVGFGADGIDLELGFWIADAAKGTASVRSA
VNRNIWRLFVAHGISIPFPQREVRVIGLPDAIAPAAGAHGPAAERQRQDA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=192, Percent_Identity=36.9791666666667, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI2367355, Length=297, Percent_Identity=30.6397306397306, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=31.0606060606061, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48517; Mature: 48517

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCHHCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure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CCEEEEECCCHHCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]