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The map label for this gene is nadE [H]

Identifier: 83718910

GI number: 83718910

Start: 1007963

End: 1009648

Strand: Direct

Name: nadE [H]

Synonym: BTH_I0882

Alternate gene names: 83718910

Gene position: 1007963-1009648 (Clockwise)

Preceding gene: 83719325

Following gene: 83721111

Centisome position: 26.46

GC content: 68.8

Gene sequence:

>1686_bases
ATGAAGACCCGTATCGCACTTGCCCAACTCAACGTCACCGTCGGCGATTTCGCCGGCAACGTCGCCAAGATCGTCGCCGC
CGCGCAAGCCGCGCACGATGCCGGCGCGCACTTCCTGATCGCGCCCGAGCTCGCGCTGTCCGGCTACCCGCCCGAGGATC
TGCTGCTGCGCCCCGCGTTCTACGCGGCGTCCGACGCGGCGCTCGCCGAGCTCGCCGCGCAACTCAAGCCGTTCGCCGGG
CTCGCGGTGCTCGTCGGCCACCCGCTGCGCGCACCAAGCGCCGATGGTAATGCAAACCGTGCGATCGAGCGCGGCGTCCC
GCCGGTCGACACGTACAACGCGGCATCGCTGATCGTCGGCGGCGAGGTCGCCGGCACGTACCGCAAGCAGGACTTGCCGA
ACACCGAGGTGTTCGACGAGAAGCGCTATTTCGCGACCGACGCCGCGCCGTACGTATTCGAGCTGAACGGCGTGAAGTTC
GGCGTCGTGATCTGCGAGGACGTGTGGCATGCGTCGGCCGCGCAGCTCGCGAAGGCGGCGGGCGCGCAGGTGCTGATCGT
GCCGAACGGCTCGCCGTACCACATGAACAAGGACGCGGTGCGCATCGACATCCTGCGCGCGCGGATTCGCGAAACGGGCC
TGCCGATGGTCTACGTGAATCTCGTCGGCGGCCAGGACGAGCTCGTGTTCGACGGCGGCTCTTTCGTGCTCGACGGCGCG
GGCGAGCTGGTCGCGAAGATGCCGCAGTTCGAGGAGGGCAATGCGATCGTCGAGTTCGACGGCGCGCGAGCGCTGCCCGC
CGCTATCGCGCCGGCGCTCAGCGTCGAGGCGCAGGTGTATCGCGCGCTCGTGCTCGGCGTGCGCGACTACATCGGCAAGA
ACGGTTTCCCCGGCGCGATCATCGGGCTGTCGGGCGGCGTCGATTCGGCGCTCGTGCTCGCGGTGGCCGTCGACGCGCTC
GGTGCCGAGCGCGTGCGCGCGGTGATGATGCCGTCGCGCTACACGGCCGGCATCTCGACGACCGACGCGGCCGACATGGC
GCGGCGCGTCGGCGTGCGCTACGACGAGATCGCGATCGCGCCGATGTTCGATGCGTTCCGCGCGTCGCTCGCGGCCGAGT
TCGCGGGCCTCGCCGAAGACGCGACGGAGGAGAACATCCAGGCGCGCATTCGCGGCACGCTGCTGATGGCGCTGTCGAAC
AAGTTCGGCTCGATCGTGCTGACGACGGGCAACAAGAGCGAGATGGCGGTCGGCTACTGCACGCTTTACGGCGACATGGC
GGGCGGCTTCGCGGTGATCAAGGACATCGCGAAGACGCTCGTCTACCGGCTCTGCCGTTACCGCAACGCGGCGGCCGAAT
ACGGCCAGCCCGACATCGTTCCCGAGCGGATTCTCACGCGCGCGCCGTCGGCCGAGCTGCGCGAGAACCAGACCGACCAG
GACAGCCTGCCGCCGTACGATGTGCTCGACGCGATCATGCGCATGTACATGGAGGAGGACCGGCCGCTCGCGGAGATCGT
CGCGGCGGGCTATTCGGAGGCGGACGTGAAGCGCGTCACGCGGCTCATCAAGATCAACGAATACAAGCGCCGGCAGGCGC
CCGTCGGCATTCGCGTCACGCACCGCGCGTTCGGGCGCGACTGGCGCTATCCGATCACGTCGCGCTTCGTCGAGAGCATC
GACTGA

Upstream 100 bases:

>100_bases
GTCTGCAAGGTCGGCTCTCGCGCGTTGCCGCGCCGCGCCGCCCGTGCGTTTGATCAAAGCCGTTCGCGCCGTGGCCCGAG
GAGGCCGCGCTGATCCCGTG

Downstream 100 bases:

>100_bases
CGCGGTGCGCGGCGTAGAATCGGCTGACGACTTTCAATCATTCGAAGCAAGGGGCACATCATGAAACGCATCACCGCCAT
CATCAAGCCGTTCAAGCTGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHFLIAPELALSGYPPEDLLLRPAFYAASDAALAELAAQLKPFAG
LAVLVGHPLRAPSADGNANRAIERGVPPVDTYNAASLIVGGEVAGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVKF
GVVICEDVWHASAAQLAKAAGAQVLIVPNGSPYHMNKDAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDGA
GELVAKMPQFEEGNAIVEFDGARALPAAIAPALSVEAQVYRALVLGVRDYIGKNGFPGAIIGLSGGVDSALVLAVAVDAL
GAERVRAVMMPSRYTAGISTTDAADMARRVGVRYDEIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSN
KFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGQPDIVPERILTRAPSAELRENQTDQ
DSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYSEADVKRVTRLIKINEYKRRQAPVGIRVTHRAFGRDWRYPITSRFVESI
D

Sequences:

>Translated_561_residues
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHFLIAPELALSGYPPEDLLLRPAFYAASDAALAELAAQLKPFAG
LAVLVGHPLRAPSADGNANRAIERGVPPVDTYNAASLIVGGEVAGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVKF
GVVICEDVWHASAAQLAKAAGAQVLIVPNGSPYHMNKDAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDGA
GELVAKMPQFEEGNAIVEFDGARALPAAIAPALSVEAQVYRALVLGVRDYIGKNGFPGAIIGLSGGVDSALVLAVAVDAL
GAERVRAVMMPSRYTAGISTTDAADMARRVGVRYDEIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSN
KFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGQPDIVPERILTRAPSAELRENQTDQ
DSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYSEADVKRVTRLIKINEYKRRQAPVGIRVTHRAFGRDWRYPITSRFVESI
D
>Mature_561_residues
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHFLIAPELALSGYPPEDLLLRPAFYAASDAALAELAAQLKPFAG
LAVLVGHPLRAPSADGNANRAIERGVPPVDTYNAASLIVGGEVAGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVKF
GVVICEDVWHASAAQLAKAAGAQVLIVPNGSPYHMNKDAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDGA
GELVAKMPQFEEGNAIVEFDGARALPAAIAPALSVEAQVYRALVLGVRDYIGKNGFPGAIIGLSGGVDSALVLAVAVDAL
GAERVRAVMMPSRYTAGISTTDAADMARRVGVRYDEIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSN
KFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGQPDIVPERILTRAPSAELRENQTDQ
DSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYSEADVKRVTRLIKINEYKRRQAPVGIRVTHRAFGRDWRYPITSRFVESI
D

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60230; Mature: 60230

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHFLIAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEHHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHEECCHH
YAASDAALAELAAQLKPFAGLAVLVGHPLRAPSADGNANRAIERGVPPVDTYNAASLIVG
HCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEC
GEVAGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVKFGVVICEDVWHASAAQLAKAA
CCCCCCHHHCCCCCCCCCCCCCCEECCCCCEEEEECCEEEEEEEECHHHHHHHHHHHHHC
GAQVLIVPNGSPYHMNKDAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDGA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEECCC
GELVAKMPQFEEGNAIVEFDGARALPAAIAPALSVEAQVYRALVLGVRDYIGKNGFPGAI
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
IGLSGGVDSALVLAVAVDALGAERVRAVMMPSRYTAGISTTDAADMARRVGVRYDEIAIA
EEECCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHEEEE
PMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEEE
TLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGQPDIVPERILTRAPSAELRENQTDQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHCCCCCC
DSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYSEADVKRVTRLIKINEYKRRQAPVGIRVT
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
HRAFGRDWRYPITSRFVESID
HHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHFLIAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEHHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHEECCHH
YAASDAALAELAAQLKPFAGLAVLVGHPLRAPSADGNANRAIERGVPPVDTYNAASLIVG
HCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEC
GEVAGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVKFGVVICEDVWHASAAQLAKAA
CCCCCCHHHCCCCCCCCCCCCCCEECCCCCEEEEECCEEEEEEEECHHHHHHHHHHHHHC
GAQVLIVPNGSPYHMNKDAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSFVLDGA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEECCC
GELVAKMPQFEEGNAIVEFDGARALPAAIAPALSVEAQVYRALVLGVRDYIGKNGFPGAI
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
IGLSGGVDSALVLAVAVDALGAERVRAVMMPSRYTAGISTTDAADMARRVGVRYDEIAIA
EEECCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHEEEE
PMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEEE
TLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGQPDIVPERILTRAPSAELRENQTDQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHCCCCCC
DSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYSEADVKRVTRLIKINEYKRRQAPVGIRVT
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
HRAFGRDWRYPITSRFVESID
HHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]