Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 83718869

GI number: 83718869

Start: 287955

End: 288836

Strand: Reverse

Name: gcvA [H]

Synonym: BTH_I0253

Alternate gene names: 83718869

Gene position: 288836-287955 (Counterclockwise)

Preceding gene: 83720090

Following gene: 284159417

Centisome position: 7.58

GC content: 71.09

Gene sequence:

>882_bases
ATGGCACGCGATCTTCCACCGTTTCCGGCGCTGAGAGCCTTCGAGGCAGCAGCAAGACATGAGAGCTTCAGCGCCGCGGG
CGAAGAGCTGCATGTGACCCACGGTGCCATCAGCCGGCAGATCGCCGCATTCGAGGCGTGGCTCGGCAAGCCGGTGTTCC
ACCGGCACGGCAAGCGGGTGAGGCTCACCGAAGAAGGCCGCCGCTATCTCGCCACCGTCGAGGCGGCATTCGACAGCATC
GCGCGCGCGACCGGCCAGTTGCGCGAGACGGGCGCGGCGCGCGTGCTGCGGATCAACGCGCTGCCGACGTTCGCGATGAA
ATGGCTGCTGCCGCGGCTGTCGCGGTTTCAGCGCGAGGTTCCGAACGTCGAGCTGAAGCTGTCGACGTCGAACGCGCCGC
TCGGCTCGCTGGACGGCTTCGACGTCGCGATCCGGCGCGGCCCGGGCCGCTGGCCGGGCTGCACGAGCGGCCGCTTTCTC
GACGAAAGCGTGATTCCCGTATGCAGCCCGGCGCTCCTGAAGCGCACGCCGATCGCGAGCGCGGACGATCTCGCGCGCCA
CGTGCTGCTGCATTCGGATACGCGCCCGGACGCGTGGCGCGAGTGGTTCGCGGCGGCGTGCGCGCCGAGGAAGGGCCGCA
AGAAGCAGTCGTTCGACCACTTCTATCTGGCGCTGCAGGCGGCCGTGGACGGCCTCGGCGTCGCGCTCGGACCGCTGCCG
CTCATCGCCGACGAACTCGCGAGCGGCCGGCTCGTGACGCCGCTGCCGGGCCCGCGCATCGCGACGCGCAGCTACTGGTG
GATCGCGCCGCGCGAGACGGCGAGCGACGCGCTCGTCGGCCGCTTCTGCGCGTGGCTTGACGCGCAGGCGCAGGCGAGCT
GA

Upstream 100 bases:

>100_bases
CATGGGAGTCCTCGTGATTTTCACGAGCCGAGATTACGCCCGTTCGCCGCGCGCTCCAAACGATTTTATCGGCGCGCCTT
TGTGATCTAGAATCACAAGC

Downstream 100 bases:

>100_bases
GCGCCCGGGTGAATTTCGGCGGACAAGCTCGCGCACGGCCAACCCGCGCGCGACTGTGCGCCTGACGGCGCGATCGTGCC
GCCCGGCGCGGCTCGATCGC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRVRLTEEGRRYLATVEAAFDSI
ARATGQLRETGAARVLRINALPTFAMKWLLPRLSRFQREVPNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPGCTSGRFL
DESVIPVCSPALLKRTPIASADDLARHVLLHSDTRPDAWREWFAAACAPRKGRKKQSFDHFYLALQAAVDGLGVALGPLP
LIADELASGRLVTPLPGPRIATRSYWWIAPRETASDALVGRFCAWLDAQAQAS

Sequences:

>Translated_293_residues
MARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRVRLTEEGRRYLATVEAAFDSI
ARATGQLRETGAARVLRINALPTFAMKWLLPRLSRFQREVPNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPGCTSGRFL
DESVIPVCSPALLKRTPIASADDLARHVLLHSDTRPDAWREWFAAACAPRKGRKKQSFDHFYLALQAAVDGLGVALGPLP
LIADELASGRLVTPLPGPRIATRSYWWIAPRETASDALVGRFCAWLDAQAQAS
>Mature_292_residues
ARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRVRLTEEGRRYLATVEAAFDSIA
RATGQLRETGAARVLRINALPTFAMKWLLPRLSRFQREVPNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPGCTSGRFLD
ESVIPVCSPALLKRTPIASADDLARHVLLHSDTRPDAWREWFAAACAPRKGRKKQSFDHFYLALQAAVDGLGVALGPLPL
IADELASGRLVTPLPGPRIATRSYWWIAPRETASDALVGRFCAWLDAQAQAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=36.271186440678, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=33.5689045936396, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=28.8659793814433, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.4794520547945, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=28.1124497991968, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=33.8129496402878, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=182, Percent_Identity=29.6703296703297, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790208, Length=169, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32169; Mature: 32038

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTEEGRRYLATVEAAFDSIARATGQLRETGAARVLRINALPTFAMKWLLPRLSRFQREV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
PNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPGCTSGRFLDESVIPVCSPALLKRTPIAS
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
ADDLARHVLLHSDTRPDAWREWFAAACAPRKGRKKQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
LIADELASGRLVTPLPGPRIATRSYWWIAPRETASDALVGRFCAWLDAQAQAS
HHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRV
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTEEGRRYLATVEAAFDSIARATGQLRETGAARVLRINALPTFAMKWLLPRLSRFQREV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
PNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPGCTSGRFLDESVIPVCSPALLKRTPIAS
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
ADDLARHVLLHSDTRPDAWREWFAAACAPRKGRKKQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
LIADELASGRLVTPLPGPRIATRSYWWIAPRETASDALVGRFCAWLDAQAQAS
HHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]