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The map label for this gene is clcA [H]

Identifier: 83718850

GI number: 83718850

Start: 1773212

End: 1774903

Strand: Reverse

Name: clcA [H]

Synonym: BTH_I1573

Alternate gene names: 83718850

Gene position: 1774903-1773212 (Counterclockwise)

Preceding gene: 83718893

Following gene: 83719771

Centisome position: 46.6

GC content: 69.74

Gene sequence:

>1692_bases
ATGGCACTCTTTTGCGCGTCAACTCCCATGCAACGCACCCGCCTGTTCCCGACCCTCACGCGCCGCACGCAGCGCATCTG
GCGCCAATACGGCGTGTTCTGGCTCGGCGCGATCGCGGTCGGCCTCACGGCCGTCACGTACGCACGGCTCATCGACTGGG
GTTACGAAGCGTTCCGCTCGCTCGAGCGCGGCCGCCCGTGGTTGCCGTTCGTGCTGACGCCCGCGGTCGCCGCGCTGTCC
GTGTGGCTCACGCGCCGCTTCTTTCGCGGCGCGGAAGGCAGCGGCATTCCGCAGGTGATCGCGACGCTGCACGCGCAGCC
GAGCGCGTTCGGCTCGCGGCTGCTGACGCTGCGCATCCTGTTCGGCAAGATCCTGATCTCGTTGCTCGGCATCATCGGCG
GCTTCACGATCGGCCGTGAAGGGCCGACCGTGCAGATCGGCGCGTCGCTGATGTTCAACCTGCGCCGCTTCTACCCGCGC
TCGAACGCGCTGATCGAACGCCAGCTCGTGCTCGCGGGCGCGGCGGCCGGCTTGTCCGCAGCGTTCAACACGCCGCTCGC
GGGCGTCGTGTTCGCGATCGAGGAACTGAGCCGCAGCTTCTCGGCGCGCGCGAGCGGCGTGCTGATCACCGCGATCATCA
TCGCCGGCGTGATCGCGCTCGGCATGAACGGCAACTACACGTACTTCGGCACGATCGAGATCGGCCAGCACTTTCCGAAA
ATGCTCGCGGCCGCCGTGTTGCTGACGGCGCTCGTCACGGGCATCGCAGGCGGGCTGTTCGGCTGGCTGCTGCTGCACAC
GGGCCGCTGGCTGCCGGCGCGGCTCCTCACGCTCTACCGCGAGCGGCCGATCGTGTTCGCAGTGGCGTGCGGTCTCGCGA
TCGCCGCCGTCGGCGCCGTGTCCGGCGGCACGACGTTCGGCAGCGGCTATGCGGAGGCGCGCGGGCTCCTCGACGGCCGC
GAACAGTTGTCCGTGCTGTATCCGTTCCTGAAAATGATCTCGATGGTCGCGTCGTATCTGCCCGGGATTCCGGGCGGGAT
CTTCGCGCCGTCGCTGTCGATCGGCGCAGGCTTCGGCAATCTGCTGCACTTCGTGTTCAGCGGCATGCATCTGCCGATGC
TGATCGCGCTCGCCATGGTCGGCTATCTTGCGGCCGTCACGCAATCGCCGATCACGTCGTTCGTGATCGTGATGGAGATG
ATCAACGGCCACGCGCTCGTGATCTCGCTGATGGCGACCGCGCTGATCGCGAGCCGCGTATCGCGCTTGTTCGTGCCGCC
GCTCTACGAAACGCTCGCCGAACGCTATCTGAAGCCGCCGCAGCCGAAGCACGCGGCGGCGGCCGCGACCGGCGCGGCTC
CGTCGCACGCCGGCATCGCGGCGGTCAAGATGCCGGAAGACGCCGAATCGGCGCCCGCCGATCCGCTCGACACGCTACGC
GACGAGGCAACGAACGACGCGCAGGCAAACGCTACGAGCGCCGCCGGCGGCAATATCGACGCCCGCGCGGCGGTGCGCGC
CGCCGATGCGCCGACGCCCGGCGGCGATGCCGCACACGCGGCCGCGCAGCCCCACGCCGGCGCGGCACCGACACCCGTCG
AGGCCGGCGAGCCGAATGCGTGCGCGGCCGGCCCGGCGTCGCAATCGGGCGACACCGCACATCGCGAGCCGCCGCATCGT
CCCGCTCAATAG

Upstream 100 bases:

>100_bases
TAGTTGGGCATTCTAAATAGACAGAACGTCACAAACACCACAAATTGCGAAGAATTTACATTTGCCCCGACCCCGGGATC
GTGGTAGTCGGGCGTCTATA

Downstream 100 bases:

>100_bases
ATGACGACGGACGCCGGCCGGTAATACACCGGCCGCGCCGGCACGACGACGCAGCCGGCCAGCGTCAGCGCGACGAGCGC
GAGGGCGAACAGGGTTCGTT

Product: voltage gated chloride channel family protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MALFCASTPMQRTRLFPTLTRRTQRIWRQYGVFWLGAIAVGLTAVTYARLIDWGYEAFRSLERGRPWLPFVLTPAVAALS
VWLTRRFFRGAEGSGIPQVIATLHAQPSAFGSRLLTLRILFGKILISLLGIIGGFTIGREGPTVQIGASLMFNLRRFYPR
SNALIERQLVLAGAAAGLSAAFNTPLAGVVFAIEELSRSFSARASGVLITAIIIAGVIALGMNGNYTYFGTIEIGQHFPK
MLAAAVLLTALVTGIAGGLFGWLLLHTGRWLPARLLTLYRERPIVFAVACGLAIAAVGAVSGGTTFGSGYAEARGLLDGR
EQLSVLYPFLKMISMVASYLPGIPGGIFAPSLSIGAGFGNLLHFVFSGMHLPMLIALAMVGYLAAVTQSPITSFVIVMEM
INGHALVISLMATALIASRVSRLFVPPLYETLAERYLKPPQPKHAAAAATGAAPSHAGIAAVKMPEDAESAPADPLDTLR
DEATNDAQANATSAAGGNIDARAAVRAADAPTPGGDAAHAAAQPHAGAAPTPVEAGEPNACAAGPASQSGDTAHREPPHR
PAQ

Sequences:

>Translated_563_residues
MALFCASTPMQRTRLFPTLTRRTQRIWRQYGVFWLGAIAVGLTAVTYARLIDWGYEAFRSLERGRPWLPFVLTPAVAALS
VWLTRRFFRGAEGSGIPQVIATLHAQPSAFGSRLLTLRILFGKILISLLGIIGGFTIGREGPTVQIGASLMFNLRRFYPR
SNALIERQLVLAGAAAGLSAAFNTPLAGVVFAIEELSRSFSARASGVLITAIIIAGVIALGMNGNYTYFGTIEIGQHFPK
MLAAAVLLTALVTGIAGGLFGWLLLHTGRWLPARLLTLYRERPIVFAVACGLAIAAVGAVSGGTTFGSGYAEARGLLDGR
EQLSVLYPFLKMISMVASYLPGIPGGIFAPSLSIGAGFGNLLHFVFSGMHLPMLIALAMVGYLAAVTQSPITSFVIVMEM
INGHALVISLMATALIASRVSRLFVPPLYETLAERYLKPPQPKHAAAAATGAAPSHAGIAAVKMPEDAESAPADPLDTLR
DEATNDAQANATSAAGGNIDARAAVRAADAPTPGGDAAHAAAQPHAGAAPTPVEAGEPNACAAGPASQSGDTAHREPPHR
PAQ
>Mature_562_residues
ALFCASTPMQRTRLFPTLTRRTQRIWRQYGVFWLGAIAVGLTAVTYARLIDWGYEAFRSLERGRPWLPFVLTPAVAALSV
WLTRRFFRGAEGSGIPQVIATLHAQPSAFGSRLLTLRILFGKILISLLGIIGGFTIGREGPTVQIGASLMFNLRRFYPRS
NALIERQLVLAGAAAGLSAAFNTPLAGVVFAIEELSRSFSARASGVLITAIIIAGVIALGMNGNYTYFGTIEIGQHFPKM
LAAAVLLTALVTGIAGGLFGWLLLHTGRWLPARLLTLYRERPIVFAVACGLAIAAVGAVSGGTTFGSGYAEARGLLDGRE
QLSVLYPFLKMISMVASYLPGIPGGIFAPSLSIGAGFGNLLHFVFSGMHLPMLIALAMVGYLAAVTQSPITSFVIVMEMI
NGHALVISLMATALIASRVSRLFVPPLYETLAERYLKPPQPKHAAAAATGAAPSHAGIAAVKMPEDAESAPADPLDTLRD
EATNDAQANATSAAGGNIDARAAVRAADAPTPGGDAAHAAAQPHAGAAPTPVEAGEPNACAAGPASQSGDTAHREPPHRP
AQ

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI55770840, Length=413, Percent_Identity=25.4237288135593, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI55770838, Length=413, Percent_Identity=25.181598062954, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1786350, Length=440, Percent_Identity=28.1818181818182, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081943, Length=309, Percent_Identity=29.4498381877023, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71996455, Length=509, Percent_Identity=23.5756385068762, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996452, Length=509, Percent_Identity=23.5756385068762, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 59033; Mature: 58902

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFCASTPMQRTRLFPTLTRRTQRIWRQYGVFWLGAIAVGLTAVTYARLIDWGYEAFRS
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERGRPWLPFVLTPAVAALSVWLTRRFFRGAEGSGIPQVIATLHAQPSAFGSRLLTLRIL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
FGKILISLLGIIGGFTIGREGPTVQIGASLMFNLRRFYPRSNALIERQLVLAGAAAGLSA
HHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AFNTPLAGVVFAIEELSRSFSARASGVLITAIIIAGVIALGMNGNYTYFGTIEIGQHFPK
HHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
MLAAAVLLTALVTGIAGGLFGWLLLHTGRWLPARLLTLYRERPIVFAVACGLAIAAVGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
SGGTTFGSGYAEARGLLDGREQLSVLYPFLKMISMVASYLPGIPGGIFAPSLSIGAGFGN
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHCCCHHH
LLHFVFSGMHLPMLIALAMVGYLAAVTQSPITSFVIVMEMINGHALVISLMATALIASRV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SRLFVPPLYETLAERYLKPPQPKHAAAAATGAAPSHAGIAAVKMPEDAESAPADPLDTLR
HHHCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHH
DEATNDAQANATSAAGGNIDARAAVRAADAPTPGGDAAHAAAQPHAGAAPTPVEAGEPNA
HHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
CAAGPASQSGDTAHREPPHRPAQ
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALFCASTPMQRTRLFPTLTRRTQRIWRQYGVFWLGAIAVGLTAVTYARLIDWGYEAFRS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERGRPWLPFVLTPAVAALSVWLTRRFFRGAEGSGIPQVIATLHAQPSAFGSRLLTLRIL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
FGKILISLLGIIGGFTIGREGPTVQIGASLMFNLRRFYPRSNALIERQLVLAGAAAGLSA
HHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AFNTPLAGVVFAIEELSRSFSARASGVLITAIIIAGVIALGMNGNYTYFGTIEIGQHFPK
HHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
MLAAAVLLTALVTGIAGGLFGWLLLHTGRWLPARLLTLYRERPIVFAVACGLAIAAVGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
SGGTTFGSGYAEARGLLDGREQLSVLYPFLKMISMVASYLPGIPGGIFAPSLSIGAGFGN
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHCCCHHH
LLHFVFSGMHLPMLIALAMVGYLAAVTQSPITSFVIVMEMINGHALVISLMATALIASRV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SRLFVPPLYETLAERYLKPPQPKHAAAAATGAAPSHAGIAAVKMPEDAESAPADPLDTLR
HHHCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHH
DEATNDAQANATSAAGGNIDARAAVRAADAPTPGGDAAHAAAQPHAGAAPTPVEAGEPNA
HHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
CAAGPASQSGDTAHREPPHRPAQ
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA