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The map label for this gene is dnaB [H]

Identifier: 83718789

GI number: 83718789

Start: 2455884

End: 2457266

Strand: Direct

Name: dnaB [H]

Synonym: BTH_I2183

Alternate gene names: 83718789

Gene position: 2455884-2457266 (Clockwise)

Preceding gene: 83718728

Following gene: 83720511

Centisome position: 64.47

GC content: 65.0

Gene sequence:

>1383_bases
ATGAACGCGCCGAAAGACCCCCAAATCGAATCGCTGAAAGTCCCGCCGCACTCGATCGAGGCCGAGCAGTCCGTGCTCGG
CGGCCTGTTGCTCGATAACGGCGCCTGGGACCGGATCGCCGACTTCCTGTCGCAGAGCGATTTCTACCGGTACGACCATC
GCGTCATTTTCGAGCACATCGGCCGGCTCATCGCGGCGACGCGCCCGGCCGACGTCGTCACCGTCTACGAGGCGCTCACG
ACGTCCGGCAAGGCGGAGGACGTGGGCGGGCTCGCGTATCTGAACGCGCTCGCGCAGAACACGCCGAGCGCCGCGAACAT
CCGCCGCTACGCGGAAATCGTCCGCGATCGCGCGGTATTGCGCCGGCTCGTGTCGGTCGCCGACGAAATCTCGGCCGATG
CGTTCAATCCGCAGGGCAAGGAAGTCCGCCAGCTGCTCGACGAGGCCGAATCGAAGGTGTTCTCGATCGCCGAGGACGGC
GCGCGCGGCACGCAGGGCTTCCTCGAGATCGGGCCGCTCCTCACGCAAGTCGTCGAGCGGATCGACACCCTCTATCACAC
CGCGAATCCGAGCGACGTGACAGGCACGCCGACGGGCTTCGTCGACCTCGACCGGATGACGTCCGGCATGCACGGCGGCG
AGCTGATCATCGTCGCGGGCCGCCCGTCGATGGGTAAGACGGCATTCTCGATGAACATCGGCGAATACGTCGCCGTCGAG
TACGGCTTGCCCGTCGCGGTGTTCTCGATGGAAATGCCGGGCACGCAGCTCGTGATGCGGATGCTCGGCTCGGTCGGCAG
GCTCGATCAGCACCGGATGCGCACCGGGCGCCTGACCGACGAGGATTGGCCGAAGCTCACGCACGCGGTGCAGAAGATGA
GCGAGGCGCAGCTCTTCATCGACGAGACGGGCGGCCTGAATCCGATGGAATTGCGCTCGCGCGCGCGGCGTCTCGCGCGC
CAGTGCGGCAAGCTCGGGCTCATCATCGTCGATTACCTGCAGCTGATGACGGGCTCGTCGCAGGGCGAGAACCGCGCGAC
CGAAATCTCGGAAATTTCTCGCTCGCTGAAGAGCCTCGCGAAGGAGCTCGACGTGCCGGTGATCGCGCTGTCGCAGCTCA
ATCGCGGGCTCGAGCAGCGGCCGAACAAGCGGCCGGTGATGTCGGATCTGCGGGAATCGGGCGCAATCGAACAGGATGCG
GACGTGATCCTGTTCATTTACCGTGACGAAGTGTACAACCCGGACAGCCCCGACAAGGGCACCGCCGAGATCATCATCGG
CAAGCAGCGTAACGGCCCGATCGGCCCCGTTCGGCTCACGTTCCTGGGGCAATACACGAAATTCGACAATTTTGCAGGGG
CGCAAAACTTCTACGGCGAGTAG

Upstream 100 bases:

>100_bases
AGGGCAGGGGCTCGGGCAACCGGTTCCTGCCTTTTTTCGTTTCTCGGCGCATCCGGCGCCGGGTGGCGAAGCCGCGCCCA
TTTCGCGATAATCCGTAGCC

Downstream 100 bases:

>100_bases
CGCCGGTTGTAAACCCCTATTTCGCAAAACGTTGTATCTCGTCGCCGACGTTGCGATCGGGCGCTTGGCGGGCAAGTGTA
AAACAGTACAATGTCGCCGG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNAPKDPQIESLKVPPHSIEAEQSVLGGLLLDNGAWDRIADFLSQSDFYRYDHRVIFEHIGRLIAATRPADVVTVYEALT
TSGKAEDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDG
ARGTQGFLEIGPLLTQVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
YGLPVAVFSMEMPGTQLVMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFIDETGGLNPMELRSRARRLAR
QCGKLGLIIVDYLQLMTGSSQGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQNFYGE

Sequences:

>Translated_460_residues
MNAPKDPQIESLKVPPHSIEAEQSVLGGLLLDNGAWDRIADFLSQSDFYRYDHRVIFEHIGRLIAATRPADVVTVYEALT
TSGKAEDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDG
ARGTQGFLEIGPLLTQVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
YGLPVAVFSMEMPGTQLVMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFIDETGGLNPMELRSRARRLAR
QCGKLGLIIVDYLQLMTGSSQGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQNFYGE
>Mature_460_residues
MNAPKDPQIESLKVPPHSIEAEQSVLGGLLLDNGAWDRIADFLSQSDFYRYDHRVIFEHIGRLIAATRPADVVTVYEALT
TSGKAEDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDG
ARGTQGFLEIGPLLTQVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
YGLPVAVFSMEMPGTQLVMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFIDETGGLNPMELRSRARRLAR
QCGKLGLIIVDYLQLMTGSSQGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQNFYGE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=453, Percent_Identity=56.5121412803532, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50685; Mature: 50685

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPKDPQIESLKVPPHSIEAEQSVLGGLLLDNGAWDRIADFLSQSDFYRYDHRVIFEHI
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
GRLIAATRPADVVTVYEALTTSGKAEDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVL
HHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGTQGFLEIGPLLTQVVER
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
IDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
HHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YGLPVAVFSMEMPGTQLVMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFI
CCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEE
DETGGLNPMELRSRARRLARQCGKLGLIIVDYLQLMTGSSQGENRATEISEISRSLKSLA
ECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNPDSPDKG
HHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
TAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQNFYGE
CEEEEEECCCCCCCCCEEEEEEECHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPKDPQIESLKVPPHSIEAEQSVLGGLLLDNGAWDRIADFLSQSDFYRYDHRVIFEHI
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
GRLIAATRPADVVTVYEALTTSGKAEDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVL
HHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGTQGFLEIGPLLTQVVER
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
IDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
HHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YGLPVAVFSMEMPGTQLVMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFI
CCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEE
DETGGLNPMELRSRARRLARQCGKLGLIIVDYLQLMTGSSQGENRATEISEISRSLKSLA
ECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNPDSPDKG
HHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
TAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQNFYGE
CEEEEEECCCCCCCCCEEEEEEECHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]