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The map label for this gene is gspO [H]

Identifier: 83718694

GI number: 83718694

Start: 1280325

End: 1281380

Strand: Reverse

Name: gspO [H]

Synonym: BTH_I1134

Alternate gene names: 83718694

Gene position: 1281380-1280325 (Counterclockwise)

Preceding gene: 83719565

Following gene: 83721162

Centisome position: 33.64

GC content: 69.32

Gene sequence:

>1056_bases
GTGCGAACCCGTCGTGATCGTCGTGCTGGGCGCGCTGATCGGCGCACTCGTCGTCGCGATGTACTTGCCGATCGTCCAGC
TCGGCAACGTGGTGTAGCATCGCAGCCGAATCCGACTCCATTTGCGATGCCAACCGCCTCGATGACGCCCAATCCGTTCC
TTCCCGGCCCGCCCGAGCACGCGGCAGCAACCGGGCCGCTCGCCGCGTTCGCGGCGCTCCCCACGGGCATGCAGCTTGCG
TTCGCGATCGTGCTCGGTCTCGTCGTCGGTAGCTTCATCAACGTCGTCGCCCACCGGCTGCCGATCATGATGAAGCGCGC
GTGGCTCGCGGAAATCGCCGAAGCGACGGGCGAGCCGTGCGCCGACGACGGCCTGCCCGCGCGCTACAACCTCTGCGTGC
CGCGCAGCGCGTGCCCGCATTGCGGCCATGTGCTGAGAGCGTGGGAAAACGTGCCGGTCCTGAGCTATATCGTATTGCGC
GGCCGCTGCCTGCGCTGCCGTGCGCCGATCGGCGTGCGCTATCCGCTCATCGAGCTCGCGAGCGGCGCGCTCGCCGCCGG
CGCGCTCGCGCTTTTCGGGCCGAGCGGCGCCGCGCTCGCCGCGTTCGGCCTGTGCGCGGCACTCCTCGCGATGAGCGCGA
TCGACATGCAGACGGGCTTTCTCCCCGATTCGCTGACGCTGCCGCTCCTGTGGGCGGGGCTGTGCGTCAATCTGTGGGGC
ACGTTTGCGAGCCTGCGCGCCGCGGTGGTCGGCGCGATCGCCGGCTATCTGTTCCTCTGGTGCATTCTGTGGCTCTTCAA
ATTGCTGCGGGGCATCGAAGGAATCGGCTATGGCGACCTGAAGCTCCTTGCCGCGCTCGGCGCATGGCTCGGCTGGGAAG
CACTGCCGCAAGTCGTGCTGATCGCCGCGGTCGCCGGCGCGGCGGTGGGCCTCGTCGCAACCTGGCGTGGCCGCATGCGC
TTCGAGGAGCCGCTGCCGTTCGGCCCGTTCCTTGCGGCGGGCGGCGCCGCGACCCTCTTTTTCGGCACGCCGTTCTACCT
GCTGCTAGGAGGCTGA

Upstream 100 bases:

>100_bases
CCGTCGCCGAGGAATCGGGCACGCTGGACACGATGCTCATCGACGTCGCGACGCTCTACGATCGCCGCGTCGACGAACAG
ATCGGCGCGCTCGCGAACCT

Downstream 100 bases:

>100_bases
ACCATGTTCTCGGTGGGATTGACGGGTGGGATCGGCAGCGGCAAAACGACCGTCTCCAATCTGTTCGGCGCGCTCGGTGC
AACGATCGTCGACACCGATT

Product: type IV pilus prepilin peptidase PilD

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRTRRDRRAGRADRRTRRRDVLADRPARQRGVASQPNPTPFAMPTASMTPNPFLPGPPEHAAATGPLAAFAALPTGMQLA
FAIVLGLVVGSFINVVAHRLPIMMKRAWLAEIAEATGEPCADDGLPARYNLCVPRSACPHCGHVLRAWENVPVLSYIVLR
GRCLRCRAPIGVRYPLIELASGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWG
TFASLRAAVVGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALGAWLGWEALPQVVLIAAVAGAAVGLVATWRGRMR
FEEPLPFGPFLAAGGAATLFFGTPFYLLLGG

Sequences:

>Translated_351_residues
MRTRRDRRAGRADRRTRRRDVLADRPARQRGVASQPNPTPFAMPTASMTPNPFLPGPPEHAAATGPLAAFAALPTGMQLA
FAIVLGLVVGSFINVVAHRLPIMMKRAWLAEIAEATGEPCADDGLPARYNLCVPRSACPHCGHVLRAWENVPVLSYIVLR
GRCLRCRAPIGVRYPLIELASGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWG
TFASLRAAVVGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALGAWLGWEALPQVVLIAAVAGAAVGLVATWRGRMR
FEEPLPFGPFLAAGGAATLFFGTPFYLLLGG
>Mature_351_residues
MRTRRDRRAGRADRRTRRRDVLADRPARQRGVASQPNPTPFAMPTASMTPNPFLPGPPEHAAATGPLAAFAALPTGMQLA
FAIVLGLVVGSFINVVAHRLPIMMKRAWLAEIAEATGEPCADDGLPARYNLCVPRSACPHCGHVLRAWENVPVLSYIVLR
GRCLRCRAPIGVRYPLIELASGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWG
TFASLRAAVVGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALGAWLGWEALPQVVLIAAVAGAAVGLVATWRGRMR
FEEPLPFGPFLAAGGAATLFFGTPFYLLLGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=279, Percent_Identity=37.6344086021505, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=47.7272727272727, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 37217; Mature: 37217

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRRDRRAGRADRRTRRRDVLADRPARQRGVASQPNPTPFAMPTASMTPNPFLPGPPEH
CCCCCHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAATGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVAHRLPIMMKRAWLAEIAEATGEPC
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADDGLPARYNLCVPRSACPHCGHVLRAWENVPVLSYIVLRGRCLRCRAPIGVRYPLIELA
CCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHH
SGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TFASLRAAVVGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALGAWLGWEALPQVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYLLLGG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCEEEEECC
>Mature Secondary Structure
MRTRRDRRAGRADRRTRRRDVLADRPARQRGVASQPNPTPFAMPTASMTPNPFLPGPPEH
CCCCCHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAATGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVAHRLPIMMKRAWLAEIAEATGEPC
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADDGLPARYNLCVPRSACPHCGHVLRAWENVPVLSYIVLRGRCLRCRAPIGVRYPLIELA
CCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHH
SGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TFASLRAAVVGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALGAWLGWEALPQVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYLLLGG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]