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The map label for this gene is outE [H]

Identifier: 83718589

GI number: 83718589

Start: 10582

End: 12075

Strand: Direct

Name: outE [H]

Synonym: BTH_I0008

Alternate gene names: 83718589

Gene position: 10582-12075 (Clockwise)

Preceding gene: 83720377

Following gene: 83721515

Centisome position: 0.28

GC content: 69.48

Gene sequence:

>1494_bases
GTGACGCAGGCGCTCGCGCAAGGCGCTCAGGATCAGCCTGCCGCGAGCGCGCCGTCGCCGCTCGCCGCGCGGCTGTTGCC
GTACGGCTTCGCGAAGGCCGGCCAGATCCTGATCGCGCATCAGCACGCGGACACGCTCGAAGTGTGGATCAGCGAGCGCA
CGAGCTCGGCCGCGCTTGCCGAAGTCGCGCGCAACTTCGGCGCGATCTCGTTGCTGCGCCTGCCGGCCGACGAACTCGCG
CAGGCGATCAACCACGCGTACGCGCGCCAGGACGGCAGCGCCGCGCAGATCGTCGGCGAGGTCGAGGGCGAAGTCGACCT
GTCGCGGCTGATGCAGGACATTCCCGAAGTCGAGGACTTGCTCGAATCGGAAGACGACGCGCCGATCATCCGGATGATCA
ACGCGCTCTTGACGCAAGCGGCGCGCGAGCAGGCGTCGGACATCCACATCGAGCCGTTCGAGAACGCATCGGTCGTGCGC
TTTCGCGTCGACGGCACGCTGCGCGACGTCGTGCGGCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGGATCAAGAT
CATGGCGCAGCTCGACATCGCGGAGAAGCGCCTGCCGCAGGACGGCCGGATCACGCTGCGCGTGGGCGGGCGGCCCGTCG
ACGTGCGGGTGTCGACGCTGCCGACCGGGCACGGCGAGCGCGCGGTGCTGCGTCTGCTGGAGAAGGACGCGCAGCGGCTG
AACCTCGAAGCGCTCGGCATGGGCCGCGACACGCTCGTGCAGTTCGACAAGCTGATCTCGCGCCCGCACGGCATCGTGCT
CGTCACGGGGCCGACCGGCTCGGGCAAGACGACGACGCTGTACGCGTCGATGTCGCGGCTCGAAACCGCGACGACCAACA
TCATGACGGTCGAGGACCCGATCGAATACGACCTCTCCGGCATCGGCCAGACGCAGGTGAACGAGCGGATCGGGATGACG
TTCGCGCGCGCGCTGCGCTCGATCCTGCGTCAGGACCCGGACATCATCATGATCGGCGAAATCCGCGATCTCGAGACCGC
GCAGATCGCGGTGCAGGCGTCGCTGACGGGCCACCTGGTGCTCGCGACGCTGCACACGAACGATGCGGCGTCGGCCGTCA
CGCGTCTGACGGACATGGGCGTCGAGCCGTACCTGCTCGCGTCGTCGCTGCTCGGCGTGCTCGCGCAGCGGCTCGTGCGC
CAGCTCTGCCCGGTGTGCAAGGAGGAGCGGCACGAGGACGGCCGCGCGGTCTGGCATCCGGTCGGCTGCGACAAGTGCGG
GCATTCGGGTTACACGGGGCGCCGCGGCGTTTATGAGCTGCTCGTCATCGACGATTCGATCCGCTCGCTGATCCACCGCA
ACGCAGCCGACGCGGAGATTCTCGCGACGGGCCGCGCGAACGGGATGCGCACGCTGCGCGACGATGCCGAACGCTGGCTC
GCGGCGGGCGCGACGTCGCTCGAGGAAGTGCTGCGCGTGACGGGAGGCGCGTAG

Upstream 100 bases:

>100_bases
ACATCGACAAGATGCGGCGCCAGCAGCTCGTTGCGCCCGCGCCCGCCAGCGCGCCCGCGGCGGCGAGCGACGCCGGCGCC
GCATCGTCGGGAGCCCGTCC

Downstream 100 bases:

>100_bases
CGTCATGCCGGCCTTCCGTTTCGAAGCGATCGACGCGTCGGGGCGCGCGCAAAAGGGCGTCATCGAGGCCGACAGCGCAC
GCAACGCGCGCGGCCAGCTG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTQALAQGAQDQPAASAPSPLAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALAEVARNFGAISLLRLPADELA
QAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVR
FRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMT
FARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVR
QLCPVCKEERHEDGRAVWHPVGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
AAGATSLEEVLRVTGGA

Sequences:

>Translated_497_residues
MTQALAQGAQDQPAASAPSPLAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALAEVARNFGAISLLRLPADELA
QAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVR
FRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMT
FARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVR
QLCPVCKEERHEDGRAVWHPVGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
AAGATSLEEVLRVTGGA
>Mature_496_residues
TQALAQGAQDQPAASAPSPLAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALAEVARNFGAISLLRLPADELAQ
AINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRF
RVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLN
LEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMTF
ARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQ
LCPVCKEERHEDGRAVWHPVGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWLA
AGATSLEEVLRVTGGA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=481, Percent_Identity=48.8565488565489, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786296, Length=440, Percent_Identity=42.9545454545455, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=36.1702127659575, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53886; Mature: 53755

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQALAQGAQDQPAASAPSPLAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALA
CCCHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEHHHHHCCCCHHHHH
EVARNFGAISLLRLPADELAQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDL
HHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHCHHHHHH
LESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGA
HCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHC
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDP
CHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEECCC
IEYDLSGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEE
LATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERHEDGRAVWHP
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
VGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
AAGATSLEEVLRVTGGA
HHCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQALAQGAQDQPAASAPSPLAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALA
CCHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEHHHHHCCCCHHHHH
EVARNFGAISLLRLPADELAQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDL
HHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHCHHHHHH
LESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGA
HCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHC
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDP
CHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEECCC
IEYDLSGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEE
LATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERHEDGRAVWHP
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
VGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
AAGATSLEEVLRVTGGA
HHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]