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The map label for this gene is purT [H]

Identifier: 83718462

GI number: 83718462

Start: 1110682

End: 1112112

Strand: Direct

Name: purT [H]

Synonym: BTH_I0979

Alternate gene names: 83718462

Gene position: 1110682-1112112 (Clockwise)

Preceding gene: 83720798

Following gene: 83719230

Centisome position: 29.16

GC content: 71.42

Gene sequence:

>1431_bases
ATGAACCGGCCGTTTCGCCGTCTGTTTCGCCGGCCGTTTCGATGCGCGGGGCGCGATGCCGAGCGCATGCGCAACGCGCG
CGGCCTGCCGCTCGCGCGCGTCAGGCCGCGCCGGCCGGCGACCCGTCGCGCGAGGCCCGGGCGACGGGCGGCGCGCGTGC
CTCGCGTGTTAGCATCGCAAGTCGTTTTCGCCATCCATCATCCAGCGGGAAATCTCATGCAGATCGGTCAGCGGCTCGGC
ACGCCGCTTTCGCCGTCCGCCACGCGCGTCATGCTGCTCGGCGCGGGCGAGTTGGGCAAGGAAGTCATCATCGCGTTGCA
GCGGCTCGGCGTCGAAGTGATCGCCGTCGACCGCTATCCGAACGCACCCGGGCATCAGGTCGCGCATCGCGCGCACGTGA
TCGACATGACGGACCCGGACGCGCTGCGCGCGCTCGTCGACGCGGAGCGTGCGCATCTCGTCGTGCCGGAAATCGAGGCG
ATTGCGACCGACGCGCTCGCCGAGATCGAAGCGGCGGGCGTCGCCGAGGTGATCCCGACCGCGCGCGCGACGCAGCTCAC
GATGAATCGCGAGGGCATCCGCCGGCTCGCGGCCGAGGAGCTTGGGCTGCCCACGTCGCCGTACGCGTTCGCGCAGTCGT
TCGACGCGTTCAGGGCGGCCGTCGCGCAGATCGGTTTTCCGTGCGTCGTGAAGCCCGTGATGTCGTCGTCGGGCAAGGGG
CAGTCGGTCGTGAGGAGCGACGCCGACGTCGAGCCCGCGTGGCAGTACGCGATGGCGGGCGGGCGCGTGAATCACGGCCG
CGTGATCGTCGAGGGCTTCGTGCAGTTCGATTACGAGATCACGCAGCTCACGGTGCGCGCGATCGATCCGGCGAGCCTCA
AGACGCGCACCTATTTCTGCGAGCCGATCGGGCATGTGCAGGTCGCGGGCGACTATGTCGAATCGTGGCAGCCGCAGCCG
ATGAGCGCGAAGGCGCTCGAACGCTCGCGCGACATCGCGCATCGCGTGACGAGCGCGCTCGGCGGCCGCGGGATCTTCGG
CGTCGAGCTGTTCGTGCGCGGCGACGATGTCTGGTTCTCCGAGGTGAGCCCGCGCCCGCACGACACGGGGCTCGTGACGC
TCGCGTCGCAGCGCCAGTCGGAGTTCGAATTGCATGCGCGCGCGATCCTCGGCCTGCCGGTCGAGCCGGCGCTCGCGACG
CCCGCCGCGTCGGCCGTGATCTACGGCGGGCTCGACGAGGCGGGCATCGCATTCGAAGGCGTGCGCGACGCGCTCGCGGT
GCCGGGCGCCGACCTGCGGCTGTTCGGCAAGCCCGAGAGCTTCGCGAAGCGCCGGATGGGCGTCGCGCTCGCGACGGGCG
CGAACGTCGACGAAGCGCGCGAGCGCGCGAAGCGGGCGGCCGCCGCGGTGCGTCCCGTGTCCGCGCGCTGA

Upstream 100 bases:

>100_bases
GCGGGCGTTGCGATGACCGCCGAGCGACGCGGCGGTCATGGGGAACGCAGGAGGCGCGAGATGGCGATCCCGGCTCCCAC
GCGTCGTAGCTGCGACTGCG

Downstream 100 bases:

>100_bases
CGAACGGCGGCGTGCGCTGCGACCGGATCGTGCCGGTCGCGCCGTGAGCCGCTGCGTTCGCAGCAGTCGTGGTTGCCGGC
CCGATGCGGCGCGCTTGCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNRPFRRLFRRPFRCAGRDAERMRNARGLPLARVRPRRPATRRARPGRRAARVPRVLASQVVFAIHHPAGNLMQIGQRLG
TPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDPDALRALVDAERAHLVVPEIEA
IATDALAEIEAAGVAEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDAFRAAVAQIGFPCVVKPVMSSSGKG
QSVVRSDADVEPAWQYAMAGGRVNHGRVIVEGFVQFDYEITQLTVRAIDPASLKTRTYFCEPIGHVQVAGDYVESWQPQP
MSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGLPVEPALAT
PAASAVIYGGLDEAGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR

Sequences:

>Translated_476_residues
MNRPFRRLFRRPFRCAGRDAERMRNARGLPLARVRPRRPATRRARPGRRAARVPRVLASQVVFAIHHPAGNLMQIGQRLG
TPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDPDALRALVDAERAHLVVPEIEA
IATDALAEIEAAGVAEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDAFRAAVAQIGFPCVVKPVMSSSGKG
QSVVRSDADVEPAWQYAMAGGRVNHGRVIVEGFVQFDYEITQLTVRAIDPASLKTRTYFCEPIGHVQVAGDYVESWQPQP
MSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGLPVEPALAT
PAASAVIYGGLDEAGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR
>Mature_476_residues
MNRPFRRLFRRPFRCAGRDAERMRNARGLPLARVRPRRPATRRARPGRRAARVPRVLASQVVFAIHHPAGNLMQIGQRLG
TPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDPDALRALVDAERAHLVVPEIEA
IATDALAEIEAAGVAEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDAFRAAVAQIGFPCVVKPVMSSSGKG
QSVVRSDADVEPAWQYAMAGGRVNHGRVIVEGFVQFDYEITQLTVRAIDPASLKTRTYFCEPIGHVQVAGDYVESWQPQP
MSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGLPVEPALAT
PAASAVIYGGLDEAGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=396, Percent_Identity=63.1313131313131, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6324702, Length=388, Percent_Identity=22.680412371134, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 51534; Mature: 51534

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPFRRLFRRPFRCAGRDAERMRNARGLPLARVRPRRPATRRARPGRRAARVPRVLASQ
CCCHHHHHHHCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VVFAIHHPAGNLMQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYP
HEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCC
NAPGHQVAHRAHVIDMTDPDALRALVDAERAHLVVPEIEAIATDALAEIEAAGVAEVIPT
CCCCHHHHHHEEEEECCCHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHCCHHHHHCC
ARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDAFRAAVAQIGFPCVVKPVMSSSGKG
CCHHEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCC
QSVVRSDADVEPAWQYAMAGGRVNHGRVIVEGFVQFDYEITQLTVRAIDPASLKTRTYFC
CHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEEHHHCCEEEEEEEEEECCCCCCCEEEEEE
EPIGHVQVAGDYVESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFS
CCCCCEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEE
EVSPRPHDTGLVTLASQRQSEFELHARAILGLPVEPALATPAASAVIYGGLDEAGIAFEG
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHH
VRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR
HHHHHHCCCCCEEEECCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNRPFRRLFRRPFRCAGRDAERMRNARGLPLARVRPRRPATRRARPGRRAARVPRVLASQ
CCCHHHHHHHCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VVFAIHHPAGNLMQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYP
HEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCC
NAPGHQVAHRAHVIDMTDPDALRALVDAERAHLVVPEIEAIATDALAEIEAAGVAEVIPT
CCCCHHHHHHEEEEECCCHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHCCHHHHHCC
ARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDAFRAAVAQIGFPCVVKPVMSSSGKG
CCHHEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCC
QSVVRSDADVEPAWQYAMAGGRVNHGRVIVEGFVQFDYEITQLTVRAIDPASLKTRTYFC
CHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEEHHHCCEEEEEEEEEECCCCCCCEEEEEE
EPIGHVQVAGDYVESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFS
CCCCCEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEE
EVSPRPHDTGLVTLASQRQSEFELHARAILGLPVEPALATPAASAVIYGGLDEAGIAFEG
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHH
VRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR
HHHHHHCCCCCEEEECCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA