Click here to switch to the map view.

The map label for this gene is otsA [H]

Identifier: 83718448

GI number: 83718448

Start: 1026780

End: 1028198

Strand: Direct

Name: otsA [H]

Synonym: BTH_I0900

Alternate gene names: 83718448

Gene position: 1026780-1028198 (Clockwise)

Preceding gene: 83720478

Following gene: 83719307

Centisome position: 26.96

GC content: 67.37

Gene sequence:

>1419_bases
ATGAGCAGATTGATCGTGGTATCGAATCGCGTGGCGGCCGGCCAGGATGCGCGCCCGACGGCGGGCGGGCTGGCGGTCGG
CGTGCTCGACGCGCTGAAGGAGACGGGCGGCGTCTGGTTCGGCTGGAACGGCGAGATCGCCGGCGCGCCCGAGGAGCCCG
CGATCGAGCGGGACGGCAACGTGACTTACGCGACCGTCGGCCTGACCCGCCGCGACTACGACCAGTACTATCGCGGCTTC
TCGAACGCCACGCTCTGGCCCGTGTTCCACTATCGCGGGGATCTCGCGCGCTTCGACCGTCAGGAATATGCGGGCTATCT
GCGCGTGAACTGCGCGCTCGCGAAGCAGTTGCGCGCGCTCGTGCAGCCCGACGACATCATCTGGGTGCACGACTATCACC
TGCTGCCGTTCGCGCACTGCCTGCGCGAGCTCGGCGTGAAGAATCCGATCGGCTTCTTCCTGCACATTCCTTTCCCGTCG
CCCGAGGTCCTGCGGACCGTACCGCCGCACGAAGAGCTGATGAAGTTCATGTGCGCGTACGACGTCGTCGGCTTGCAGAC
GGAGGGCGACAAGCAGGCGTTCGTCGATTACATCGAGCGCCGCGGGCTCGGCACGTCGAGCGAGGACGGGATTCTGCATG
CGCACGGCCGCGTCGTGAAGGTCGCCGCGTATCCGATCGGCATCTATCCGGACGCGATCGGCAAGGCGGCCGTCGAATAC
GGCTCGCGCAAGGCGGTGAAGGCGCTGCGCGACACGATGCGCGAGCGTAAGCTCGTGATCAGCGTCGACCGACTCGACTA
TTCGAAGGGCCTCGTCGAGCGCTTTCAGGCGTTCGAGCGGATGCTCGCGAACGCGCCCGGCTGGCAGGGCCGCGTGTCGC
TCGTGCAGATCGCGCCGCCGACCCGCTCCGACGTGCAGACCTATCAGCGGATCCGCCAGACGCTCGAGGGCGAGGCGGGG
CGCATCAACGGCCGCTTCGCGCAACTCGACTGGACGCCGATCCAGTACCTGAACCGCAAGTACGAGCGCAATCTGCTGAT
GGCGCTGTTCAGGCTGTCGCAGGTTGGCTATGTGACGCCGCTGCGCGACGGGATGAACCTCGTCGCGAAGGAGTACGTCG
CGTCGCAGGATCCGAACGATCCGGGCGTGCTCGTGCTGTCCGAGTTCGCGGGCGCCGCGGCCGAACTGCCGGGCGCGCTG
CTCGTGAATCCGTTCGATCTGTCGCAGATGGCCGAATCGCTCGAGCGCGCGCTTGCGATGCCGCTCGAGGAGCGGCAGGC
GCGTCACGCGGAGAACATCGCGCGGCTACGCAAGAACGATCTGTCGGTGTGGCGCGATTCGTTCCTCGCGGATCTGCGCA
GTGTCGCGACGGCCGCGTCCGTCACGCAGCGCGCGGGCAGGCGTGTCGCGAATGCTTGA

Upstream 100 bases:

>100_bases
AACGTTTGACACGTCGCGAGGTGGCATGCGACTTGCCCGATTCGACGGGAATGCCGTTTTCCCGCTCGCCGGGCAATCTG
ATACAGGAGGGTAAGCCGTA

Downstream 100 bases:

>100_bases
CGCGGGCGTCGACGCGAGCACGGACGGCGCCCGGGCCGACGCGGCGCTCGCGCGCTTTTTCGTGATCACGGGCGGGCCCG
GCTCGGGCAAGAGCACGTTG

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSRLIVVSNRVAAGQDARPTAGGLAVGVLDALKETGGVWFGWNGEIAGAPEEPAIERDGNVTYATVGLTRRDYDQYYRGF
SNATLWPVFHYRGDLARFDRQEYAGYLRVNCALAKQLRALVQPDDIIWVHDYHLLPFAHCLRELGVKNPIGFFLHIPFPS
PEVLRTVPPHEELMKFMCAYDVVGLQTEGDKQAFVDYIERRGLGTSSEDGILHAHGRVVKVAAYPIGIYPDAIGKAAVEY
GSRKAVKALRDTMRERKLVISVDRLDYSKGLVERFQAFERMLANAPGWQGRVSLVQIAPPTRSDVQTYQRIRQTLEGEAG
RINGRFAQLDWTPIQYLNRKYERNLLMALFRLSQVGYVTPLRDGMNLVAKEYVASQDPNDPGVLVLSEFAGAAAELPGAL
LVNPFDLSQMAESLERALAMPLEERQARHAENIARLRKNDLSVWRDSFLADLRSVATAASVTQRAGRRVANA

Sequences:

>Translated_472_residues
MSRLIVVSNRVAAGQDARPTAGGLAVGVLDALKETGGVWFGWNGEIAGAPEEPAIERDGNVTYATVGLTRRDYDQYYRGF
SNATLWPVFHYRGDLARFDRQEYAGYLRVNCALAKQLRALVQPDDIIWVHDYHLLPFAHCLRELGVKNPIGFFLHIPFPS
PEVLRTVPPHEELMKFMCAYDVVGLQTEGDKQAFVDYIERRGLGTSSEDGILHAHGRVVKVAAYPIGIYPDAIGKAAVEY
GSRKAVKALRDTMRERKLVISVDRLDYSKGLVERFQAFERMLANAPGWQGRVSLVQIAPPTRSDVQTYQRIRQTLEGEAG
RINGRFAQLDWTPIQYLNRKYERNLLMALFRLSQVGYVTPLRDGMNLVAKEYVASQDPNDPGVLVLSEFAGAAAELPGAL
LVNPFDLSQMAESLERALAMPLEERQARHAENIARLRKNDLSVWRDSFLADLRSVATAASVTQRAGRRVANA
>Mature_471_residues
SRLIVVSNRVAAGQDARPTAGGLAVGVLDALKETGGVWFGWNGEIAGAPEEPAIERDGNVTYATVGLTRRDYDQYYRGFS
NATLWPVFHYRGDLARFDRQEYAGYLRVNCALAKQLRALVQPDDIIWVHDYHLLPFAHCLRELGVKNPIGFFLHIPFPSP
EVLRTVPPHEELMKFMCAYDVVGLQTEGDKQAFVDYIERRGLGTSSEDGILHAHGRVVKVAAYPIGIYPDAIGKAAVEYG
SRKAVKALRDTMRERKLVISVDRLDYSKGLVERFQAFERMLANAPGWQGRVSLVQIAPPTRSDVQTYQRIRQTLEGEAGR
INGRFAQLDWTPIQYLNRKYERNLLMALFRLSQVGYVTPLRDGMNLVAKEYVASQDPNDPGVLVLSEFAGAAAELPGALL
VNPFDLSQMAESLERALAMPLEERQARHAENIARLRKNDLSVWRDSFLADLRSVATAASVTQRAGRRVANA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=464, Percent_Identity=51.7241379310345, Blast_Score=486, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI115533560, Length=448, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI115533558, Length=448, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=435, Percent_Identity=27.816091954023, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319602, Length=466, Percent_Identity=33.9055793991416, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6320279, Length=421, Percent_Identity=28.7410926365796, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323537, Length=389, Percent_Identity=29.305912596401, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323917, Length=374, Percent_Identity=29.144385026738, Blast_Score=173, Evalue=5e-44,
Organism=Drosophila melanogaster, GI19920676, Length=485, Percent_Identity=36.701030927835, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52714; Mature: 52583

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVVSNRVAAGQDARPTAGGLAVGVLDALKETGGVWFGWNGEIAGAPEEPAIERDGN
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCC
VTYATVGLTRRDYDQYYRGFSNATLWPVFHYRGDLARFDRQEYAGYLRVNCALAKQLRAL
EEEEEECCCHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHCCEEEEHHHHHHHHHHH
VQPDDIIWVHDYHLLPFAHCLRELGVKNPIGFFLHIPFPSPEVLRTVPPHEELMKFMCAY
CCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHH
DVVGLQTEGDKQAFVDYIERRGLGTSSEDGILHAHGRVVKVAAYPIGIYPDAIGKAAVEY
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHHHHHH
GSRKAVKALRDTMRERKLVISVDRLDYSKGLVERFQAFERMLANAPGWQGRVSLVQIAPP
CCHHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TRSDVQTYQRIRQTLEGEAGRINGRFAQLDWTPIQYLNRKYERNLLMALFRLSQVGYVTP
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LRDGMNLVAKEYVASQDPNDPGVLVLSEFAGAAAELPGALLVNPFDLSQMAESLERALAM
CHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCHHHHCCCCEEECCCCHHHHHHHHHHHHCC
PLEERQARHAENIARLRKNDLSVWRDSFLADLRSVATAASVTQRAGRRVANA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLIVVSNRVAAGQDARPTAGGLAVGVLDALKETGGVWFGWNGEIAGAPEEPAIERDGN
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCC
VTYATVGLTRRDYDQYYRGFSNATLWPVFHYRGDLARFDRQEYAGYLRVNCALAKQLRAL
EEEEEECCCHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHCCEEEEHHHHHHHHHHH
VQPDDIIWVHDYHLLPFAHCLRELGVKNPIGFFLHIPFPSPEVLRTVPPHEELMKFMCAY
CCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHH
DVVGLQTEGDKQAFVDYIERRGLGTSSEDGILHAHGRVVKVAAYPIGIYPDAIGKAAVEY
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHHHHHH
GSRKAVKALRDTMRERKLVISVDRLDYSKGLVERFQAFERMLANAPGWQGRVSLVQIAPP
CCHHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TRSDVQTYQRIRQTLEGEAGRINGRFAQLDWTPIQYLNRKYERNLLMALFRLSQVGYVTP
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LRDGMNLVAKEYVASQDPNDPGVLVLSEFAGAAAELPGALLVNPFDLSQMAESLERALAM
CHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCHHHHCCCCEEECCCCHHHHHHHHHHHHCC
PLEERQARHAENIARLRKNDLSVWRDSFLADLRSVATAASVTQRAGRRVANA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA