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The map label for this gene is ascD [H]

Identifier: 83718430

GI number: 83718430

Start: 909071

End: 910102

Strand: Reverse

Name: ascD [H]

Synonym: BTH_I0792

Alternate gene names: 83718430

Gene position: 910102-909071 (Counterclockwise)

Preceding gene: 83721590

Following gene: 284159416

Centisome position: 23.89

GC content: 66.76

Gene sequence:

>1032_bases
ATGGCTTTTAACGTAACCCTCAAGCAAAGCGGCCGGCAGTTCCAGGTCGAGGCCGACGAAACCGTGCTCGCGGCGGCGCT
GCGTCAGAACGTCCATCTGCCCTACGGCTGCAAGAACGGCGCGTGCGGATCGTGCAAGGGCACGATCGTGCAAGGCCAGT
TCGAGCAGGGGCCGCATTCCGCATCGGCGCTGTCGAACGACGAGCGCACGCGCGGCCTCGCGCTCCTGTGCTGCTCGAAG
CCGCAGAGCGACCTCGAAGTCGATGTGCGCGAGATCGCGGGCGTCGACGGCGTCCAGGTGAAGAAGCTGCCGTGCCGCGT
CGCCGCGCTCGAGCGCCGCGCGGACGACGTGATCGTGCTGAGGCTGCAGTTGCCCGCGAACGAGCGCCTGCAGTATCTGG
CCGGCCAGTACATCGAGTTCATCCTGAAGGACGGCACGCGCCGCAGCTATTCGATGGCGACCGCGCCGCACGAGGAAGGG
CCGATCGAGCTGCACATCCGCCACATGCCGGGCGGCAAATTCACCGACCACGTGTTCGGCGCGATGAAGGAGCGCGACAT
CCTGCGCTTCGAGGGCCCGCTCGGCACGTTCTTCCTGCGCGAAGATTCGGACAAGCCGATCGTGCTGCTCGCCTCCGGCA
CGGGCTTCGCGCCGATCAAGGCGATCATCGAGCACGCGCACCACGTGAAGCTCGCGCGCCCGATGACGCTCTATTGGGGC
GCGCGCCGCAAGAAAGATCTGTATCTGCTCGACGCCGCCGAGCAATGGGCGAAGGAAATCCCGAACTTCAAGTTCGTGCC
GGTGCTCTCCGAGCCCGACGCGAGCGACGCATGGACCGGCCGCACGGGCTTCGTGCACCGCGCGGTGATCGAGGATCTGC
CCGATCTGTCCGGCCACCAGGTGTACGCATGCGGCGCGCCGGTGATGGTCGAATCCGCGCAGCGCGACTTCACGCAACAT
CATGGCCTGCCCGCCGACGAGTTCTACGCGGACTCGTTTACGAGCGCCGCGGACCTCGCGCATCCGGTCTGA

Upstream 100 bases:

>100_bases
GATTCGTGTCGCAATCATGGTGTATCGGTTGATCCGTAGGAATGCATTGTAGCCGCCGTGCACGCGTTGCACGGTCCCCA
CTTTTTGTTCTCAACGACTT

Downstream 100 bases:

>100_bases
ACGCCGCGCGGCCCCGGAAAAACCGCCGCGACACGGAATTGTCGCGTCGCAGCGGTTTACTTGGGGCCGCGTGATGCCTT
ATGCTTGCGCACATGAACCG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAFNVTLKQSGRQFQVEADETVLAAALRQNVHLPYGCKNGACGSCKGTIVQGQFEQGPHSASALSNDERTRGLALLCCSK
PQSDLEVDVREIAGVDGVQVKKLPCRVAALERRADDVIVLRLQLPANERLQYLAGQYIEFILKDGTRRSYSMATAPHEEG
PIELHIRHMPGGKFTDHVFGAMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHAHHVKLARPMTLYWG
ARRKKDLYLLDAAEQWAKEIPNFKFVPVLSEPDASDAWTGRTGFVHRAVIEDLPDLSGHQVYACGAPVMVESAQRDFTQH
HGLPADEFYADSFTSAADLAHPV

Sequences:

>Translated_343_residues
MAFNVTLKQSGRQFQVEADETVLAAALRQNVHLPYGCKNGACGSCKGTIVQGQFEQGPHSASALSNDERTRGLALLCCSK
PQSDLEVDVREIAGVDGVQVKKLPCRVAALERRADDVIVLRLQLPANERLQYLAGQYIEFILKDGTRRSYSMATAPHEEG
PIELHIRHMPGGKFTDHVFGAMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHAHHVKLARPMTLYWG
ARRKKDLYLLDAAEQWAKEIPNFKFVPVLSEPDASDAWTGRTGFVHRAVIEDLPDLSGHQVYACGAPVMVESAQRDFTQH
HGLPADEFYADSFTSAADLAHPV
>Mature_342_residues
AFNVTLKQSGRQFQVEADETVLAAALRQNVHLPYGCKNGACGSCKGTIVQGQFEQGPHSASALSNDERTRGLALLCCSKP
QSDLEVDVREIAGVDGVQVKKLPCRVAALERRADDVIVLRLQLPANERLQYLAGQYIEFILKDGTRRSYSMATAPHEEGP
IELHIRHMPGGKFTDHVFGAMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHAHHVKLARPMTLYWGA
RRKKDLYLLDAAEQWAKEIPNFKFVPVLSEPDASDAWTGRTGFVHRAVIEDLPDLSGHQVYACGAPVMVESAQRDFTQHH
GLPADEFYADSFTSAADLAHPV

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=234, Percent_Identity=31.1965811965812, Blast_Score=125, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322146, Length=181, Percent_Identity=30.3867403314917, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322699, Length=220, Percent_Identity=23.1818181818182, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323552, Length=219, Percent_Identity=25.1141552511416, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323510, Length=214, Percent_Identity=25.7009345794392, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 37884; Mature: 37752

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNVTLKQSGRQFQVEADETVLAAALRQNVHLPYGCKNGACGSCKGTIVQGQFEQGPHS
CEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
ASALSNDERTRGLALLCCSKPQSDLEVDVREIAGVDGVQVKKLPCRVAALERRADDVIVL
HHHCCCCCCCCCEEEEEECCCCCCCEEEHHHHCCCCCCEEECCCHHHHHHHCCCCCEEEE
RLQLPANERLQYLAGQYIEFILKDGTRRSYSMATAPHEEGPIELHIRHMPGGKFTDHVFG
EEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHH
AMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHAHHVKLARPMTLYWG
HHHCCCEEEEECCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHEECCCCEEEEEC
ARRKKDLYLLDAAEQWAKEIPNFKFVPVLSEPDASDAWTGRTGFVHRAVIEDLPDLSGHQ
CCCCCCEEEEECHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCE
VYACGAPVMVESAQRDFTQHHGLPADEFYADSFTSAADLAHPV
EEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
AFNVTLKQSGRQFQVEADETVLAAALRQNVHLPYGCKNGACGSCKGTIVQGQFEQGPHS
EEEEEECCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
ASALSNDERTRGLALLCCSKPQSDLEVDVREIAGVDGVQVKKLPCRVAALERRADDVIVL
HHHCCCCCCCCCEEEEEECCCCCCCEEEHHHHCCCCCCEEECCCHHHHHHHCCCCCEEEE
RLQLPANERLQYLAGQYIEFILKDGTRRSYSMATAPHEEGPIELHIRHMPGGKFTDHVFG
EEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHH
AMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHAHHVKLARPMTLYWG
HHHCCCEEEEECCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHEECCCCEEEEEC
ARRKKDLYLLDAAEQWAKEIPNFKFVPVLSEPDASDAWTGRTGFVHRAVIEDLPDLSGHQ
CCCCCCEEEEECHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCE
VYACGAPVMVESAQRDFTQHHGLPADEFYADSFTSAADLAHPV
EEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]